FastQCFastQC Report
Sat 25 May 2024
EGAF00002434121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125676766
Total Bases18.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCCCATCTCGTA3805100.3027687711187603Illumina Paired End PCR Primer 2 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)