FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002434434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4559181
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC70105815.37684070889048No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2089584.583235453911569TruSeq Adapter, Index 1 (100% over 50bp)
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG422970.9277324150982381No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA396640.869980814536646No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA310910.6819426559287731No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC250800.5500988006398518No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC247950.543847677905308No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC226880.49763323719764585No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT216620.47512919535328824No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC208680.4577137867524891No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC205620.45100205497434737No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG199900.4384559419772981No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG198590.435582618895806No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC197140.43240222311858206No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC189310.41522808592157234No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT186520.40910856577091365No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG183940.40344965466385296No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA180820.3966063203018262No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC177550.38943397948008646No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA173580.3807262751796869No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC167200.36673253375990117No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG150150.32933546617254283No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC140260.30764297359547693No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC139980.30702882820401295No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT132480.2905785052183715No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC131370.2881438574164965No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT128820.2825507476013784No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC127300.27921681547628835No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA127010.27858073632084357No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT124660.2734263017853426No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG122680.26908341651713324No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG121810.2671751790507988No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG112120.24592136175335No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT110720.24285063479603025No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT108830.2387051534036486No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA103850.22778213894118263No Hit
GCCACGCCACTCACCTGTGGCTGGCTTCCGGAAGCCTCTCAGGAGCGGGG100940.22139941362275375No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT99310.2178242100938743No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG97840.2145999467886886No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC97590.2140516026891672No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG97170.21313038460197128No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC96460.21157308735933056TruSeq Adapter, Index 1 (98% over 50bp)
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC95550.2095771148370727No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA95460.20937971096124502No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT95100.20859009545793422No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA93620.20534389838876765No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA93040.20407174007787804No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT91420.20051847031297945No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC89630.19659232656040634No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG87320.19152562708082876No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA83940.18411201485529965No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA83890.1840023460353954No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA83710.18360753828374No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA82530.18101935413399908No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC82240.18038327497855428No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC81530.17882597773591352No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG81140.17797056094066016No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG81060.17779509082881334No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC81050.1777731570648325No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG79920.17529464173499582No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG79120.17353994061652742No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG79110.17351800685254654No Hit
GATTGAAGAAGACGTCCACCTCGTCTGGAGGCAGCAGCTGCGCGGGTTCC77960.17099562399474816No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT77270.16948219428006916No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG76960.16880224759666265No Hit
GTTGTTTTAAAAGGACAAAAAAGACAGTAATGAGTATCTAATGACTTACA76290.16733268540994534No Hit
GTCCCCACGCGGAAGGCAACTGCCTGAGAGGCGCGGCGTCGCACCGCCCA76140.1670036789502325No Hit
GATTTCTCTAATAGCTGCCACATTAGGACCTGCTCCTAGATGGGTATAAA75250.1650515739559364No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA74980.1644593623284533No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT73840.1619589132346358No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG73460.16112543020336328No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA72740.15954619919674168No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA72450.1589101200412969No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC71860.15761602796642643No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG71090.15592712813990056No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT70710.15509364510862808No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT70400.15441369842522154No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT69400.152220322027136No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG68600.1504656209086676No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC67510.14807484063475435No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC67420.14787743675892664No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG67290.14759229782717553No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT66610.14610080187647737No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG65860.14445576957791323No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC65710.1441267631182004No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC64890.14232819447177025No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC64570.1416263140243829No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA64570.1416263140243829No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA64340.14112183745282322No Hit
GAACAGTGCATGGGCCTATCAGGTTCAGTTTTCTCCACTGGGCCAGCTCC64150.14070509593718697No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG64040.14046382453339756No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC63960.14028835442155072No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG63920.1402006193656273No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA63910.14017868560164645No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA63380.1390161961106611No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT63000.13818271307938862No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT61710.13535325752585828No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT60650.1330282785438876No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT60120.13186578905290228No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG59560.13063749826997437No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC59340.13015495546239556No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT58760.12888279715150594No Hit
GTCCCTGGACAGCAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGG58500.1283125192880037No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC58450.12820285046809943No Hit
GACAGATAACGTACCTCTTCCACTTCGACCGACAAACCTGAGGTCATTAA58330.12793964530032917No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT58200.12765450636857803No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA58070.12736936743682692No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG57730.12662361946147782No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG57540.1262068779458416No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT56780.12453991188329659No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT56640.12423283918756461No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG56560.12405736907571777No Hit
GTCTGAGGCCAGCAAGAAGAAGCGGCGGGAGCCCCTCGGCGAGGACTCCG56500.12392576649183262No Hit
ATTTGTCTCAATTAAGAGTGGGCTCTTTAACACCATTGTTTTAAAAAAAT56130.123114217224541No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA55700.12217106537336421No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA55560.12186399267763222No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA55510.12175432385772797No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT54480.11949514616769986No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA53530.11741143858951861No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA53370.11706049836582491No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG52950.11613928027862899No Hit
ACTTACATACAGTTTCTTGCAGCCAAGTCTCTGTGGACAAACTTTTTGCT52160.11440651292414142No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG52120.11431877786821801No Hit
GAAATTAAGAAAGTCTGTGTTATCTTTTTATAGATATTGAAGCACAGATT51590.11315628837723266No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT50940.11173059371847706No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT50860.11155512360663024No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA50600.11098484574312797No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA50370.11048036917156831No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT50330.11039263411564489No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG50270.11026103153175976No Hit
GTCCTCTTCACCCTCACATTGCTCCCCAGGTGGCAGTTTTGGCACAGGAA50160.11001976012797035No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA50080.1098442900161235No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC49680.10896693945688929TruSeq Adapter, Index 1 (98% over 50bp)
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG49420.10839666159338705No Hit
CAAGTAGCATGTCGCCCAAGAGCAGAAGTCAAACCATACCCAATAGAGTC49290.10811152266163594No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC49010.10749737727017197No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG48810.10705870199055488No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT48460.10629102025122494No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA48350.10604974884743555No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC48150.10561107356781843No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT47500.10418537890906282No Hit
AGTTCAGTGCAAAACTTAATTTTAATATTAAAATTTTTCTCATGCCCTAT47250.10363703480954145No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC47240.1036151010455606No Hit
GCAACAGACTTTAAAGAAGTTGTGTTTTACAATGCAGAGAGTGGAGGATG46520.10203587003893902No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC46500.10199200251097731No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG46350.10166299605126447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)