FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002434461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932290
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT38315813.066852187198402No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG147360.5025423815516201Illumina Single End PCR Primer 1 (100% over 50bp)
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA134810.4597430677047632No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC111920.3816812116127668No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC96920.33052665323006936No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC94800.3232968089786481No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT94270.32148934791579276No Hit
GGGGCCACACTTACTCTGCACGGCCTCGATCTTGTAGGGGATGTTGAGGC92940.3169536437391936No Hit
GGAGAGCCGAGCCAGGCAGACGTGCAGCCACTGGGCCCCAGCAGCCTGGC84830.28929607917361516No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA84260.28735220595507266No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA71760.24472340730282474No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA66440.22658059059642807No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG65920.22480723257249455No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC64990.22163564995276727No Hit
GGACAGGAAGAGAATTAACCAACCGGCACTCACTTCCATTTTCATTGCCC64200.21894150987794522No Hit
GGGACCCGAGCCGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA63120.215258381674391No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG62400.21280296287202152No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT62290.21242782944388172No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA55870.19053367845608724No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT55830.19039726630040002No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT55730.19005623591118206No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC55530.18937417513274607No Hit
GCTGCTCCATGGTCTCAATGATGCTCTTTTGCTGCACATTATCACAGCTT54820.1869528593692984No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC53950.18398589498310194No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT51530.17573295956402676No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG51200.17460755927960742No Hit
GGAAGACACCTTGTGCGTCGGCAGACACAGCCGCATGCAGCGGGGTGCGG49730.16959441255810306No Hit
GGGGAGGGTCCCCACGCCTGGCCTGGCCCCCCTCACCGGCCCCCGCCCTC49330.16823029100123113No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA49310.16816208492338752No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT49130.16754823022279514No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC47880.16328535035757036No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC47830.16311483516296138No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA45090.15377060249838864No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT44910.15315674779779628No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT44410.15145159585170634No Hit
GTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTG44090.1503602986062088No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA43670.14892797097149327No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG43210.14735923118109057No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC43060.14684768559726358No Hit
GTGTGCCTCCCACAGCATGACCTACCATCATTGGAAAGCAGTTTGTAGTC42750.1457904913906878No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA42540.14507432757333005No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC42350.14442636983381588No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT41830.14265301180988238No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGCGGGGGG40990.13978835654045133No Hit
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA40390.13774217420514343No Hit
GGAGGGGTGACCCAAACACTGATTCTGCTCAGACATCGTCGTGCACAAGC39500.13470700374110336No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT37900.12925051751361563No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG37680.12850025065733606No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG37600.12822742634596168No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA37450.12771588076213472No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT37040.12631765616634097No Hit
AGGCCACCAACACAAATCTGAATACTGAGAGGAGAAAGATACACACACAC37010.1262153470495756No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA36550.12464660725917287No Hit
GGTAGCCTTAGAAAGCTGGGCTGACCTAAGGGCAGAGTTGGTGTTCTGCA36310.1238281343250497No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC36160.12331658874122273No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG35530.12116809728914943No Hit
GGAACATTCTCCAACACTTCCACATGCAATTCTTCTCCAGTAAGCCAGGA35180.1199744909268865No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT34890.11898550279815434No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG34840.11881498760354535No Hit
GGGTCCTGCCCTTGCACAGATGGGGTCTCATTCTGCCCCATCCCCATAGG33640.11472262293292955No Hit
CTCCCTGCCCCCCAGCAGAGGTTCTAGACGCTGGAGCTGACCTTGGCTAT33060.11274464667546526No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC33050.11271054363654344No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG32990.11250592540301267No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG32880.11213079197487288No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT32780.1117897615856549No Hit
GGATTCACATTAACTCTATGACCATATTTTATTCCAGACACTTCTGAGAA32660.11138052511859332No Hit
ATCCAGGTTTCTGTTGTTACAGTCTCTCTTCTGCCTCCTAGGCCGTTGGC32660.11138052511859332No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG32600.11117590688506251No Hit
GGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAAGCCAAGCCAA32600.11117590688506251No Hit
AGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGATACTCACCTGCAG32570.11107359776829713No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC32540.11097128865153175No Hit
GGAAAATGGCTGCTGCACCCCCAAGGAGGGCCGAGGAGCCCCTGCAGAAG32530.11093718561260993No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG31420.10715174829229032Illumina Single End PCR Primer 1 (98% over 50bp)
GCTGTGCCTTCAGGGTTCTCGGGCTGCCACGCCGATGGCGAGAGCGGCAG31240.10653789359169796No Hit
GGGACACCGATGGGCCTGCATCTGACCTGTTGTGCTCACTGCTTAGGACC31140.10619686320247997No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA31090.10602634800787097No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG31010.1057535236964966No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC30870.10527608115159141No Hit
GAGGGAGGTCATACCCTCCACATCGGGAGGGGGGCGGCCGTAGCTCATAG30730.10479863860668624No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA30710.10473043252884263No Hit
AGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACACCCAGCT30590.10432119606178106No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT30580.10428709302285927No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT29940.10210449853186418No Hit
GCTGCTCTTCCTCCTCTCCCCTACGTCTCTTGCTCCTAGATCTTCACGCT29710.10132012863666282No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT29580.10087678913067942No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC29530.10070627393607044No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA29440.10039934658577426No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG29440.10039934658577426No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)