FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002434502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652048
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC72252227.243926203447298No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT26992610.178020910632085TruSeq Adapter, Index 14 (97% over 44bp)
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA244330.921288000820498No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC199700.7530029622389941No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC195030.7353939295216376No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT173270.6533441325345544No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC158060.5959922294015795No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT151500.5712566288392971TruSeq Adapter, Index 14 (97% over 41bp)
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC149630.5642054744107196No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG135680.5116046165076952No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG132890.5010844449270903No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA123070.46405645750001506No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA113980.4297810597696573No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC107330.4047060988338069No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG96490.3638320271729622No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT95890.3615696246825095No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG94760.35730876665882366No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT86500.32616302570692535No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG83140.31349357176039044No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC82720.3119098900170736No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG82580.3113819961026347No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA82200.3099491411920146No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC81840.308591699697743No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA80400.30316193372065664No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC79680.3004470507321134No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG79490.29973062327680344No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT77740.29313194934631653TruSeq Adapter, Index 14 (97% over 41bp)
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA75970.28645786199948114No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC75290.2838938058436348No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT74970.2826871911820601No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA73870.27853945328289687No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA72770.27439171538373364No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT71990.2714505921461452No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG70550.2660208261690588No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC69650.26262722243337977No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT68340.25768764366255814No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG68310.2575745235380355No Hit
CTCAATTGTCTCCTCTACTTTAGATCGGAAGAGCACACGTCTGAACTCCA67710.25531212104758283Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA67430.25425633321870494No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA62440.23544068583977365No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC60250.22718291674962143No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC59900.22586318196352403No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC59260.22344995264037454No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC57930.21843496045320446No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT57590.2171529323752813No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC56740.21394786218047335No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT56500.2130429011842923No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC54730.20636881383745692No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT53490.20169318202385478No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA53210.20063739419497686No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG52220.19690443008572997No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC51510.194227253805361No Hit
GTCCCTGGACAGCAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGG50950.19211567814760516No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG50700.1911730104432499No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT49840.18793023354026775No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG49520.186723618878693No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC49220.18559241763346665No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT49160.1853661773844214No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG48940.18453662980458876No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG48930.18449892309641452No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG48850.18419726943102085No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTACAAGACCGCATCTT48700.1836316688084077No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA48570.18314148160214294No Hit
GTTCAGCCTAGAAGATATCCAACCCCACAAGAAAAATAAACTGGTTAATG48520.1829529480612719No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA48100.18136926631795502No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA47770.18012494494820608No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT47540.17925769066019923No Hit
CCACAGCTGTGCCCAGTGGGGCACTGGGGCCGCGCGTGCCCCCCTCACTG47250.1781641961231471No Hit
ACACACACACACACACACACACACACACACACACACACACACACAGATCG46950.17703299487792076No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG46400.17495912592833918No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG46250.174393525305726No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT46040.17360168443406757No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG45670.17220653623162174No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT45610.17198029598257647No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA45380.17111304169456962No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA45370.1710753349863954No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT45000.1696801867839496No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA42670.16089452377935845No Hit
GAAAGGAACAGGTATTTAGCATTGCAGCTAGTTTACTGGCATTATCAGCA42610.16066828353031318No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT42280.1594239621605642No Hit
GCAAGTGACCCTTGTTTTGTTTTGGTTGGGGTGGGGGGTGTTTGGGATGG42040.15851900116438314No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC41930.15810422737446683No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT41910.1580288139581184No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT41460.15633201209027892No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA41240.15550246451044625No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT41040.15474833034696203No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA40340.1521088607747673No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA39710.14973333815979198No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA38410.14483146609714456No Hit
GCCAAGTCCTACAGACTCCAACTTCTACCGTGCCCTGATGGATGAAGAAG37260.14049519465711027No Hit
CCACAAGAGGTCCCAAACCCCCCAGTACCTGTGTGGTTGCCCTCAGAGGC37230.14038207453258764No Hit
AACGGTGTTACGGCATAAATGTAGTCTTTTCCCATTTCACCAATATATTC36980.1394394068282324No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT36780.13868527266474814No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT36440.13740324458682499No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC35970.13563102930263707No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC34810.13125705115442857No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG34730.1309553974890349No Hit
ACCCCATCTGCTTCTTTCACGCAGGCAAGTCCGCCCTGCACTGGGCCGCC34080.1285044614577112No Hit
GCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGAC33720.12714701996343958No Hit
AAACTTTCTCAAATAAATTCATTTCCATTTTAATGTTATTTATTCCCAGG33570.12658141934082642No Hit
CAACACCTACGAAGGAAACATCATGAAGAATAAAGAGAAACTGCAGAGCC33560.1265437126326522No Hit
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC33440.12609123213456166No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG33250.12537480467925166No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT32580.12284845523157953No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC32500.12254680156618583No Hit
GCAACAGACTTTAAAGAAGTTGTGTTTTACAATGCAGAGAGTGGAGGATG32490.12250909485801162No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT32270.12167954727817898No Hit
ATGATGATAGGTTTTACCCATCCACTCACAAGCCGGGGGATATTTTTGCA31890.12024669236755894No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC31690.11949255820407473No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA31470.1186630106242421No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA30820.11621207459291838No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC29870.11262993731636832No Hit
ACAGTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGT29870.11262993731636832No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC29860.11259223060819412No Hit
GCAAGTAGTACAGATAGGTTAGCACCATGCTTTGTATTTTATTTAAAAGA29780.11229057694280044No Hit
GAAAACCAAAGCCTTTTTCATGTTAGATGGGATCCTTTCCAAATACTTTG29450.11104625557305148No Hit
CATTTCTTTCATCCAGCCATGTTATCCCTTATTTGATCCACTCGAAGAGC27910.10523942251422297No Hit
AATAATAATACTGCCTACCTCTTAATGAAAAGGCACCCTAGAAGAAGAAC27780.10474923530795822No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA27730.10456070176708718No Hit
GTCATTGCCAATCATACGCATAAATTCCTAAAACAAACCCACTTTCAATA27700.10444758164256454No Hit
GCTCGTCCCGCCCGCAGCCCTAGGCGGCGTCGCCGCAGCCGATCCCGGAG27560.1039196877281256No Hit
ACTTGTTTATATTACTTTCTCCAGTTTTATAAACAGTTCAATAATTTTCT27290.10290160660742188No Hit
CACCGGGCCCTGGCCAGCCTCACCTTGCCAGCCCGTGGGGCAGACACAGG27170.10244912610933136No Hit
CTCTTCCTGTCCAGGGATCACTGGAACTTGGGGCAGATACTGGTACTTTG27120.10226059256846029No Hit
AAACCTCTCATAATGAAGGCCCCCGCTGTGCTTGCACCTGGCATCCTCGT26960.10165728523767292No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT26740.10082773765784027No Hit
GCCCAGCACCCGCCTGAGCCTCTCCCTGTTGCCCAGATCCTGATCCGGAA26550.10011131020253028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)