FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002434506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953657
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC26885928.192421384208366No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT444724.663311861602232TruSeq Adapter, Index 16 (97% over 40bp)
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA74770.7840345113599543No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC62060.6507580817841215No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA61750.6475074371603208No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC60660.6360777512250212No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT59120.6199293876100107No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC58410.612484362826467No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG55680.5838577182362212No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC55040.5771467099806324No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA52780.5534484620780847No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC40250.4220595035741362No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG39700.41629223085448963No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT38100.39951471021551777No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG38050.3989904126955499No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT36570.3834712061045009No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT35530.37256581768916913No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG35360.3707832061212784No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG34960.3665888259615354No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC34360.360297255721921No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG33800.3544251234982808No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG32240.3380670408752833No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA32100.3365990078193732No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT32050.33607471029940533No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA30520.3200312061883885No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC30410.3188777516444592No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC29790.31237646239685757No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT29690.31132786735692186No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA29500.3093355367810439No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA29180.3059800326532496No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT27780.2912997020941492No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG27030.2834352392946311No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG26810.28112833020677247No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC26780.2808137516947917No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC26160.27431246244719015No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA25850.27106181782338934No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC25660.26906948724751145No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA25440.26676257815965276No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT25210.2643508095678006TruSeq Adapter, Index 16 (97% over 40bp)
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA24820.2602612889120512No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT24640.25837381784016683No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC24600.25795437982419256No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT23900.25061421454464233No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA22030.23100548729784398No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC21570.22618195011413958No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC21230.22261672697835802No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT21210.22240700797037088No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG21140.2216729914424159No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG20510.21506684269082071No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC20090.21066274352309058No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG20000.2097190079871484No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA19330.20269342121957892No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA19260.2019594046916239No Hit
GTCCCTGGACAGCAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGG19030.19954763609977172No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC18950.19870876006782312No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT18880.1979747435398681No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG18660.19566783445200947No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT18600.19503867742804804No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG18310.19199775181223439No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA18260.1914734542922665No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG18160.19042485925233077No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT18120.19000542123635647No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG18020.1889568261964207No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC17790.18654505760456852No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT17620.18476244603667777No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA17580.18434300802070347No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA17500.18350413198875487No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT17480.1832944129807677No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG17350.18193123942885125No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG17210.18046320637294122No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC16970.17794657827709545No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT16960.17784171877310187No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA16940.1776319997651147No Hit
GAAAGGAACAGGTATTTAGCATTGCAGCTAGTTTACTGGCATTATCAGCA16840.17658340472517897No Hit
CCACAGCTGTGCCCAGTGGGGCATTGGGGCCGCGCGTGCCCCCCTCACTG16500.17301818158939744No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA16490.17291332208540386No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA16210.1699772559735838No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT16120.16903352043764164No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT16060.16840436341368017No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT16010.1678800658937123No Hit
GCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGAC16000.1677752063897187No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA15910.1668314708537766No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT15880.16651689234179584No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA15330.16074961962214926No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC15000.1572892559903613No Hit
AACGGTGTTACGGCATAAATGTAGTCTTTTCCCATTTCACCAATATATTC14590.1529900163266248No Hit
CCACAAGAGGTCCCAAACCCCCCAGTACCTGTGTGGTTGCCCTCAGAGGC14570.15278029731863763No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT14530.15236085930266333No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG14490.15194142128668903No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT14420.151207404758734No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC14390.1508928262467533No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT14310.1500539502148047TruSeq Adapter, Index 16 (97% over 40bp)
GCAAGTGACCCTTGTTTTGTTTTGGTTGGGGTGGGGGGTGTTTGGGATGG14070.1475373221189589No Hit
AAACTTTCTCAAATAAATTCATTTCCATTTTAATGTTATTTATTCCCAGG13650.1431332229512288No Hit
GCAACAGACTTTAAAGAAGTTGTGTTTTACAATGCAGAGAGTGGAGGATG13530.1418749089033059No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA13370.1401971568394087No Hit
ACCCCATCTGCTTCTTTCACGCAGGCAAGTCCGCCCTGCACTGGGCCGCC13330.13977771882343443No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC13120.13757566923956938No Hit
CAACACCTACGAAGGAAACATCATGAAGAATAAAGAGAAACTGCAGAGCC13060.1369465122156079No Hit
ATGATGATAGGTTTTACCCATCCACTCACAAGCCGGGGGATATTTTTGCA13020.1365270741996336No Hit
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC12930.13558333866369146No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC12720.13338128907982638No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT12430.13034036346401273No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG12420.13023550396001918No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC12140.12729943784819908No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA12120.12708971884021195No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA11970.12551682628030833No Hit
AAATAAGACTGTCCTCATGGCTCTGTGACTGTGCCTCTTGTCAGGTGTAT11800.12373421471241756No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC11630.1219516031445268No Hit
GAAAACCAAAGCCTTTTTCATGTTAGATGGGATCCTTTCCAAATACTTTG11350.11901553703270672No Hit
GCAAGTAGTACAGATAGGTTAGCACCATGCTTTGTATTTTATTTAAAAGA11330.11880581802471957No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT11310.11859609901673242No Hit
ACAGTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGT10890.11419199984900233No Hit
CTCTTCCTGTCCAGGGATCACTGGAACTTGGGGCAGATACTGGTACTTTG10840.11366770232903445No Hit
ACACACACACACACACACACACACACACACACACACACACACACAGATCG10730.11251424778510513No Hit
GCTTATAAAAATGTGTGGGTAATCTGCTTACCTGTCAGCAATACGACCAA10680.11198995026513725No Hit
GTTCTAATTGCACTGTTTTTTCTCATTCCTTCCCCAGGCTGGATGATAGA10670.11188509076114367No Hit
GTCATTGCCAATCATACGCATAAATTCCTAAAACAAACCCACTTTCAATA10560.11073163621721438No Hit
AATAATAATACTGCCTACCTCTTAATGAAAAGGCACCCTAGAAGAAGAAC10530.11041705770523365No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA10260.10758585109740715No Hit
CATTTCTTTCATCCAGCCATGTTATCCCTTATTTGATCCACTCGAAGAGC10160.1065372560574714No Hit
AAACCTCTCATAATGAAGGCCCCCGCTGTGCTTGCACCTGGCATCCTCGT10150.10643239655347783No Hit
ACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCC10120.1061178180414971No Hit
ATTACTTTTCCAAGGACGGTTGAAGAAATTGCTTGCTTCCACTAAAAATG10050.10538380151354208No Hit
ACACTGGAAATTCCAACATGCCTGGGGGGCTCCCAAGGCAAGCTTACACC10010.10496436349756778No Hit
GTTTTACTAGTGGTCCTTTGGCGTGCTCCTCTGGGAGCTGATGACAAGAG10000.1048595039935742No Hit
AAAACAAAATGAAATGAATGGTGCTTACTTCAAGCAAAGCTCAGTGTTCA9950.10433520647360633No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC9820.10297203292168988No Hit
CAGCCAGTGGGAAGCTTTACCTGATGTCCTTGGGCAGTTCTGGCAACTGC9790.10265745440970915No Hit
GCAAAGGAAAAGGTCTAGGAGAATATGTAAATTTAACATTGATTAACTGA9740.10213315688974128No Hit
AAAACTCCACCATTCAGAGTGCTTTTTCCTTCTGAAGGAAGCTGAGGAAC9730.1020282973857477No Hit
AAAAGGCAGCTTGGACACGGCTTTACCTCCAATGGTGCAGGCTCCAAGTA9720.10192343788175413No Hit
GACATGGCCAACTGCCCCACCCCAAGTCTGGAGAGGCCGGTGGGAGAAGC9700.10171371887376698No Hit
GCTCGTCCCGCCCGCAGCCCTAGGCGGCGTCGCCGCAGCCGATCCCGGAG9680.10150399986577983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)