FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002434550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009576
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC51885125.818928968100735No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC26413413.143767640537106TruSeq Adapter, Index 11 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC243001.2092103010784365TruSeq Adapter, Index 11 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC141730.7052731521475177TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAACACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC78820.3922220408683225TruSeq Adapter, Index 11 (98% over 50bp)
ACACACACACAAACACACACACACACACACACACACACACACACACACAC67120.3340008041497311No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA53800.26771816542395016No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG51620.2568701059327938No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA49790.24776370736911668No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG46630.23203899728101848No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG45100.224425450940895No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC37520.1867060514257734No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC36080.179540360752716No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC35650.17740060589895582No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA34080.16958801259569184No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA33750.16794587514978285No Hit
ACACACACACAGACACACACACACACACACACACACACACACACACACAC33630.1673487342603614No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC32630.16237256018184928No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC32060.15953614095709742No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG31420.15635138954684968No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC30630.15242021202482514No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT29840.1484890345028006No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT29110.14485642742548677No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC29060.14460761872156117No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG28160.14012906205090028No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC27890.13878549504970203No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT27840.1385366863457764No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC27700.13784002197478473No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC27210.1354016966763138No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT26670.1327145626739173No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC26420.13147051915428926No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC26160.1301767138938761No Hit
CCACAGCTGTGCCCAGTGGGGCACTGGGGCCGCGCGTGCCCCCCTCACTG25940.12908195559660346No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG25560.12719100944676887No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG25390.12634505985342181No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG25290.1258474424455706No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC25240.125598633741645No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG24260.12072198314470317No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC23790.11838318132780248No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG23680.11783580217916616No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC23680.11783580217916616No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA23060.11475057425048867No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC22910.11400414813871183No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG22760.11325772202693503No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG22690.1129093898414392No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT22620.11256105765594335No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC22490.11191415502573678No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC22460.1117648698033814No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT22110.11002320887590218No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA22100.10997344713511706No Hit
GATCGGAAGACCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC21780.10838107142999319TruSeq Adapter, Index 11 (98% over 50bp)
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG21650.10773416879978663No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT21590.1074355983550759No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT21510.10703750442879494No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG20740.10320585038834064No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG20630.10265847123970429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)