FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002434554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378208
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC19607214.2265898906406No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT684604.9673198820497335TruSeq Adapter, Index 20 (97% over 44bp)
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA202451.4689364740300448No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG173881.2616383013304233No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC109690.7958885741484595No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC109660.7956709001834267No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG100180.7268859272330447No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT96790.7022887691843321No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC83820.6081810583017948No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC80450.5837290162297708No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC80210.581987624509508No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG77580.5629048735749611No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC69010.5007226775639091No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG59980.43520281408901995No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC56460.4096624021918317No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG53850.3907247672339734No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT52580.3815099027142492No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA49150.35662251271215956No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT47240.3427639369384012No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA47040.3413127771715155No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC45620.33100954282662703No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC45490.3300662889781513No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC41890.3039454131742088No Hit
GATTGAAGAAGACGTCCACCTCGTCTGGAGGCAGCAGCTGCGCGGGTTCC41020.2976328681882561No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG40940.29705240428150176No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT40860.2964719403747475TruSeq Adapter, Index 20 (97% over 41bp)
GCCACGCCACTCACCTGTGGCTGGCTTCCGGAAGCCTCTCAGGAGCGGGG40610.2946579906661404No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT38980.282831038566022No Hit
GTCCCCACGCGGAAGGCAACTGCCTGAGAGGCGCGGCGTCGCACCGCCCA38250.27753430541688917No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA37390.2712943184192807No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC37310.2707138545125264No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA36730.2665054911885579No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT36710.26636037521186934No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG36530.26505433142167223No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA35810.2598301562608837No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT35780.2596124822958509No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG35090.2546059811000952No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC34670.2515585455896352No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG34430.24981715386937242No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT34270.24865622605586385No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG34040.2469873923239453No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC33140.24045717337295966No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG33060.23987670946620537No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT32880.23857066567600826No Hit
GAACAGTGCATGGGCCTATCAGGTTCAGTTTTCTCCACTGGGCCAGCTCC31510.22863022127284127No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT30960.2246395319139056No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA30780.22333348812370846No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA30770.22326093013536416No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG30760.22318837214701992No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC30140.21868977686967422No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA30020.21781908100954284No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA28940.20998281826836007No Hit
ATCCTCCGTGGTCATGCTCCAATAAATTCACTGCTTTGTGGCGCGACCCT28600.2075158466646544No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG28500.20679026678121154No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC27940.20272701943393162No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA27700.20098562771366876No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT26890.1951084306577817No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG26780.19431029278599457No Hit
GATTTCTCTAATAGCTGCCACATTAGGACCTGCTCCTAGATGGGTATAAA26460.19198843715897745No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC25960.1883605377417632No Hit
GACAGATAACGTACCTCTTCCACTTCGACCGACAAACCTGAGGTCATTAA25710.1865465880331561No Hit
GTCTGAGGCCAGCAAGAAGAAGCGGCGGGAGCCCCTCGGCGAGGACTCCG25220.18299124660428615No Hit
ACTTACATACAGTTTCTTGCAGCCAAGTCTCTGTGGACAAACTTTTTGCT24820.18008892707051474No Hit
ATCCAGGTTTCTGTTGTTACAGTCTCTCTTCTGCCTCCTAGGCCGTTGGC24770.17972613712879332No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT24740.17950846316376048No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG24560.17820241937356335No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG24450.17740428150177623No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG24440.17733172351343193No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG24230.17580800575820196No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA24100.17486475190972625No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG23970.17392149806125054No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT23670.17174475841092202No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA22950.16652058325013352No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC22710.16477919152987067No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC22630.16419872762311638No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT22430.1627475678562307No Hit
GCAAGAGGGAAAACATGGCACGGGTAGGTCATTAGCCACACTAATTTAAC21810.15824897257888507No Hit
GTCCTCTTCACCCTCACATTGCTCCCCAGGTGGCAGTTTTGGCACAGGAA21630.15694292878868793No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT21400.15527409505676937TruSeq Adapter, Index 20 (97% over 41bp)
GACAGAGAGCAAGTTTTCTGTGGCCTCAGATGTTTGGAGCTTTGGAGTGG21040.15266200747637512No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG21020.15251689149968656No Hit
ATTTGTCTCAATTAAGAGTGGGCTCTTTAACACCATTGTTTTAAAAAAAT20920.1517913116162437No Hit
CCAACGTCTCCGACTGGTCCGAGGGCGTCTCCAGCCCTCCCACCAGCATG20850.1512834056978337No Hit
ATACAAAGAGCTAAATAGGCGAATTGATCTAAAACAGATACATTCAAATT20550.14910666604750517No Hit
CACCTGACTGTTTTCTCTTTTCCACGTTGAGTACACAGAACTAGACACAT20540.14903410805916087No Hit
CACAAGAAAATCATCAGAGTGCAGGACACTTTGTGGTCACTTGGCCACTT20520.1488889920824723No Hit
GCCAGATAGCTGTGAAACAGGCACTGGTCTTGCCAGGATTGAGGCCACCC20480.14859876012909518No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC20310.14736527432724233No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT19940.1446806287585038No Hit
CAAGTAGCATGTCGCCCAAGAGCAGAAGTCAAACCATACCCAATAGAGTC19900.14439039680512666No Hit
AACCTCATTTCCCCATAAAGATTCAGAACACAAAGATCATCCTTTCAGAG19610.1422862151431424No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG19390.14068993939956814No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG19300.1400369175044696No Hit
CTTCACAGGTGCTTTCAAGAACAGGAGCAGAAGTCACAACAAGCTTCAGT19050.13822296779586246No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA18690.13561088021546822No Hit
CTCCCTGCCCCCCAGCAGAGGTTCTAGACGCTGGAGCTGACCTTGGCTAT18670.13546576423877965No Hit
CAAGCTCTGTGCAGGTGCCACCCTCCTGGCAGCAGAGCTCCCAGGGTTTA18630.1351755322854025No Hit
GACCGACACGTGCCACCCCTTCAGGACCCAACTGCGAGATCAACCTGGAT18590.13488530033202534No Hit
ACAGCGAGATCCTGGCCGTCGGGCGCCCTCGGCCCCAGGACGGTGGCCGG18580.13481274234368107No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT18510.13430483642527108No Hit
GTATCAAGTACCTTTGCTGAGCAAGTTGCGCAGCTTGTTGACCAGACATA18480.13408716246023822No Hit
AGTTCAGTGCAAAACTTAATTTTAATATTAAAATTTTTCTCATGCCCTAT18390.13343414056513966No Hit
GCACAAGTTAGGTTTGTTTTGTTTTGCCTCACAGTTTGCTGTTTGTCTCC18370.1332890245884511No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA17840.129443451206204No Hit
AGCTGGCTCGGCTCTTGATCTCCCTGCAACCCCCGTCCCCAGGGCCGGCC17630.12791973345097402No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC17580.1275569435092526No Hit
GCCTTTTAGGTGATGTGGCAGTGAAAATGTTGAATGTGACAGCACCTACA17580.1275569435092526No Hit
CTCATCCTGGTGTGGGAAGGAATCCCGCTGTCTCTGGCTGACAAACTCTA17560.12741182753256403No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG17500.1269764796024983No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC17500.1269764796024983No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG17290.12545276184726833No Hit
GAAATTAAGAAAGTCTGTGTTATCTTTTTATAGATATTGAAGCACAGATT17170.12458206598713692No Hit
GTCTCCCTGAAAGAGTCCACTTGGAAAGATTCCTTTTAGTTCTGCCAGCA17100.12407416006872693No Hit
GTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGCTGA17070.12385648610369407No Hit
CCCACTCCTGGCCTGCCCAAACTCAGGGTTTCCTTCTCGCTGATTCCTTG16950.12298579024356267No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC16830.12211509438343124No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA16740.12146207248833268No Hit
GCAAGGGCAGGACCCAGAGCCTGTCCTGGGCAGCTCCGGCCTCTCCCTCC16730.12138951449998839No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT16480.11957556479138126No Hit
GATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAA16330.118487194966217No Hit
CCTCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGT16320.11841463697787272No Hit
GTTGTTTTAAAAGGACAAAAAAGACAGTAATGAGTATCTAATGACTTACA16150.11718115117601988No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT16110.11689091922264273No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC16040.11638301330423274No Hit
GAAAGAGAACTTCACTTACATGCAGTTTTTCCAAAAGAATTAAATAATCG15710.11398859968887134No Hit
CCTTGCAAATGTTCACTGACAGCAGCACGGTGGAAAGCATCTCGCTCCAG15640.11348069377046135No Hit
AGCCTGCCTCAGGAAACTGTGGATCAGGAACCCAAGGATCAGAAGAGGAA15600.11319046181708423No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC15470.11224720796860851No Hit
CACTACGTTGTTTATATAAACACTACGTTGTTTATATATTTCACTTTATA15330.11123139613178853No Hit
CTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCTTTGAGTTCTACCGC15090.1094900044115257No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA14920.10825651860967285No Hit
AAGCAGTGAGCACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTC14920.10825651860967285No Hit
GCTACTCTGCCCCATGCCACACTAGGAGTGCCAGAGTTCCCAGGCAACAA14880.1079662866562957No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC14750.10702303280782002No Hit
GTTTTTAATTTCATTTTTAAAAACAATAATAGGAAAGCAATTATTTACAA14560.10564443102927859No Hit
GCATCTCTACCACAGAACCGCCACTGATTTTTTTGGACATTTTGACTTCA14500.1052090830992129No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG14330.10397559729736004No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA14260.10346769137895007No Hit
AAACACTTCCTTCTTTGTGGATCTCTTTTTTCTCTTTTCTGTGAGAATAC14250.10339513339060577No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG14240.10332257540226147No Hit
ACCTGGCCTCACTTTCAGGATGAATTTTTCCGACAGCACTGACTTGCCCT14090.10223420557709721No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT14080.10216164758875294No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA14080.10216164758875294No Hit
CAACGCAAGGGGATTAAAGAAATAACCTCCTACCCCTCCAGAAGGTTGCA14030.1017988576470315No Hit
GCTTTACATTAAATTTACCTGCCAACTGTTTAGCCTGGCTTGCGTTTTCA13980.10143606770531008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)