FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002434555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378208
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT18960013.756994590076388No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA204641.4848266734774431No Hit
GGGGCCACACTTACTCTGCACGGCCTCGATCTTGTAGGGGATGTTGAGGC176971.2840587197288071No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT114030.827378741089879No Hit
GGAGAGCCGAGCCAGGCAGACGTGCAGCCACTGGGCCCCAGCAGCCTGGC104100.7553286586640042No Hit
GCTGCTCCATGGTCTCAATGATGCTCTTTTGCTGCACATTATCACAGCTT104010.7546756367689057No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC97840.709907357960482No Hit
GGAGGGGTGACCCAAACACTGATTCTGCTCAGACATCGTCGTGCACAAGC83540.6061494346281548No Hit
GGGGAGGGTCCCCACGCCTGGCCTGGCCCCCCTCACCGGCCCCCGCCCTC82920.6016508393508092No Hit
GTGTGCCTCCCACAGCATGACCTACCATCATTGGAAAGCAGTTTGTAGTC78600.5703057883860781No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG74750.5423709628735285Illumina Single End PCR Primer 1 (100% over 50bp)
GGACAGGAAGAGAATTAACCAACCGGCACTCACTTCCATTTTCATTGCCC69040.500940351528942No Hit
GGGACCCGAGCTGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA64270.4663301910887181No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC59980.43520281408901995No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC59600.43244561053193714No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG57130.4145237874108988No Hit
GTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTG52620.38180013466762636No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA52460.3806392068541178No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT50010.362862499709768No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA49240.35727553460725814No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT48080.34885880795932106No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC48000.3482783440525668No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC45980.3336216304070213No Hit
GGTAGCCTTAGAAAGCTGGGCTGACCTAAGGGCAGAGTTGGTGTTCTGCA41810.30336494926745455No Hit
GCTGTGCCTTCAGGGTTCTCGGGCTGCCACGCCGATGGCGAGAGCGGCAG41770.3030747173140774No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT41630.30205890547725744No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG41190.2988663539901089No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA37710.2736161740462978No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT37260.270351064570805No Hit
AGGCCACCAACACAAATCTGAATACTGAGAGGAGAAAGATACACACACAC36930.2679566509554436No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA36630.2657799113051151No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC36540.2651268894100165No Hit
GAGGGAGGTCATACCCTCCACATCGGGAGGGGGGCGGCCGTAGCTCATAG35750.25939480833081796No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT35450.25721806868048946No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG35080.2545334231117509No Hit
GGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAAGCCAAGCCAA34920.25337249529824235No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC34370.2493818059393067No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA33680.24437530474355101No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT33440.2426339130232882No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG32750.23762741182753255No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA32530.2360311360839583No Hit
GGGCGGCTACTCCTGCAAGGTGGGGGTCCCTCCTAGGGTAAGGGTTGTGG32450.23545067217720403No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG32370.23487020827044977No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT31410.2279046413893984No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG31110.22572790173906987No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG30830.22369627806542988No Hit
GAGCCGCCGCCGCCGGCCATGGAGGTGGCGCCCGAGCAGCCGCGCTGGAT30650.22239023427523277No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC29420.21346560170888573No Hit
GGAAAATGGCTGCTGCACCCCCAAGGAGGGCCGAGGAGCCCCTGCAGAAG29340.21288513780213145No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT29050.21078095614014722No Hit
GGAGTTTTAGAAGAACACCACCACTACCCCAACCAAAGTAACACAACACT28980.2102730502217372No Hit
GAGGATGCTCGGCCAGGTCCCACCTCCCACCGGGGACCCAGAAGCAGGGG28870.20947491234995008No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT28430.20628236086280158No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA28030.20338004132903018No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC27360.19851865610996305No Hit
GGAACATTCTCCAACACTTCCACATGCAATTCTTCTCCAGTAAGCCAGGA27350.1984460981216188No Hit
GGTGTTTAGGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCAC27000.1959065685295688No Hit
GGGACCCGAGCCGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA26890.1951084306577817No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG26780.19431029278599457No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT26430.1917707631939446No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC26340.19111774129884604No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT26270.19060983538043605No Hit
GTAGTTGGGGGTTAAGCTTTGTGGATGTAGCCTTTATATTTAGTATAATT26150.18973913952030463No Hit
GTGCAGGCTGGCGCCCTGGTAGATGAAGTCGGAGATGACGGCCGGCGCGT25980.18850565371845177No Hit
GCAGAATCCAACTGTAAAAGATCTTATTGGCTTTGGTCTTCAAGTAGCCA24930.1808870649423019No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG24900.18066939097726903No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT24490.17769451345515336No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA24330.17653358564164479No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT24290.17624335368826766No Hit
GGTCGGCCCTGGCATCCACAGAGCGCACACAGACGCCCGAAGGCTTGGGA23580.17109173651582346No Hit
GGACTTTTACTCTTCTCAATGTATGTGAAAAGTTCATACAGAACCACTCC22990.16681081520351065No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT22960.1665931412384778No Hit
GGATAGAGATGAAGATCTTAATTATAGCTATGCAGCATAGATTTAGTCAA22890.1660852353200678No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC22480.16311035779795213No Hit
CGAGACAAGGAGCAAACACGAGATCTTGTGCCCCCAACACAGCACTATCT22360.16223966193782072No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC22070.16013548027583646No Hit
ATCCAGGTTTCTGTTGTTACAGTCTCTCTTCTGCCTCCTAGGCCGTTGGC21870.15868432050895076No Hit
GGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTTCATTCAAGG21790.15810385660219647No Hit
GTGTCTGTGTCCAAAGCAAGCTATCTTCTTAGGGAAGGGGAAGAATTCAC21470.15578200097517936No Hit
GCACAAGTTAGGTTTGTTTTGTTTTGCCTCACAGTTTGCTGTTTGTCTCC21370.1550564210917365No Hit
GTCTCCCTGAAAGAGTCCACTTGGAAAGATTCCTTTTAGTTCTGCCAGCA21280.15440339919663795No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG21250.15418572523160512No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA21130.15331502937147368No Hit
AGGTGACATGTATTTTGCTCTGTTCAATTTGAGTTATGTCTGTTTTTAAG21120.15324247138312938No Hit
GTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGCTGA21070.15287968144140798No Hit
AAGCAGTGAGCACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTC20580.14932434001253803No Hit
GGGATTTGTCACTGGATGGAGGGAGTCAAAACTTGGGACTGCCTTGCAAT20260.1470024843855209No Hit
GAGCACAGTGCAGTCAGCCCCCACGTGCAGGGCCGCTCACCTGTGTAGCC20250.1469299263971766No Hit
GAGAGACCAATACATGAGGACAGGCGAAGGCTTCCTCTGTGTATTTGCCA20240.14685736840883234No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC20130.14605923053704523No Hit
GAGGTCAATGAGTGCAACAGCAACCCCTGCGTCCACGGGGCCTGCCGGGA20110.14591411456035663No Hit
GTATCAAGTACCTTTGCTGAGCAAGTTGCGCAGCTTGTTGACCAGACATA19900.14439039680512666No Hit
GGAGGCCCTACTGAGGCGCCAGGCCTGGCCCGGCTGGGCCCCGCGGGCCG19870.1441727228400938No Hit
ACAGTCATCGTGACAGAACGCACCGCAGCCTTGGCACATGATCATGGCTT19730.1431569110032738No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC19580.14206854117810955No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA19520.14163319324804385No Hit
GCACAGCAGCCAGTAGAATTCCCCTTTTTATTTTTTTTTCCCCGCAGACC19020.1380052938308296No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA18930.13735227193573105No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC18730.13590111216884534No Hit
GGTACTTGTGGGGATTCTGACATGCTGTCATCGTGGGCTGGTGGAAGAAC18640.1352480902737468No Hit
GGGAACCAGGAGCTAATAAAAATAACTTCTTTCTCTGGAAAAGAGTAATT18500.13423227843692678No Hit
CTCCCTGCCCCCCAGCAGAGGTTCTAGACGCTGGAGCTGACCTTGGCTAT18480.13408716246023822No Hit
GGTGTTTCTGATGTACCAACCTCACCAACATTACAGAGGCCTACTCATAT18340.13307135062341824No Hit
GAAGAACTAATAAAAAATTATGATGGGAAAGTACACGGGGATAGAGGTGA18340.13307135062341824No Hit
GCAAGGGCAGGACCCAGAGCCTGTCCTGGGCAGCTCCGGCCTCTCCCTCC18190.13198298079825396No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC18110.1314025168914997No Hit
GCAGCAATTGATGTCACGTATCCAGCAAATCTGCTGTCACTTCCACCATA18070.13111228493812255No Hit
AGGTACAGCTCCAGAGAGTAGAACAGGGCAGGCACAGGGCCCTTACCCTG17980.130459263043024No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG17880.12973368315958114No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG17840.129443451206204No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG17740.12871787132276113No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT17670.12820996540435114No Hit
CAAGTAGCATGTCGCCCAAGAGCAGAAGTCAAACCATACCCAATAGAGTC17670.12820996540435114No Hit
GGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCGCGAGATGTCCCT17450.12661368966077688No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT17440.1265411316724326No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG17430.12646857368408831No Hit
GGTTATCATGTGAAACAAAACTCACCTGGGTCACTTTGATATGGTCTTGG17370.12603322575402262No Hit
ATACAAAGAGCTAAATAGGCGAATTGATCTAAAACAGATACATTCAAATT17290.12545276184726833No Hit
GAAAGAGAACTTCACTTACATGCAGTTTTTCCAAAAGAATTAAATAATCG17290.12545276184726833No Hit
GGAGGCCACAACAGCCTCACCTGCAGCAGTTGTTGTTTCCGCACATGAGC17160.12450950799879264No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA16740.12146207248833268No Hit
CTTCACAGGTGCTTTCAAGAACAGGAGCAGAAGTCACAACAAGCTTCAGT16540.12001091272144698No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC16400.11899510088462699No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT16390.11892254289628272No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA16340.1185597529545613No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA16260.117979289047807No Hit
GACCCCAAGGCGGGCATCATGATCAAAAGTGTGAAACGCGCCTACCATCG15890.11529464347906848No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA15770.11442394761893705No Hit
GACCCACTCTGTCTCCGCAGAGGCCACAGGCCAGGTCTGCCATGCCTTGT15610.1132630198054285No Hit
GAGAGACAGGATCAGGTCAGCGGGCTACCACTGGGCCTCACCTCTATGGT15530.11268255589867422No Hit
GGTTATAATGTGAAACAAAACTCACCTGGGTCACTTTGATATGGTCTTGG15510.11253743992198566No Hit
GCATCTCTACCACAGAACCGCCACTGATTTTTTTGGACATTTTGACTTCA15440.11202953400357565No Hit
GTTGAGGGCAATGAGGACATAACCAGCCACCTCCTGGATGGTCTAAGAAG15430.11195697601523137No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG15430.11195697601523137No Hit
CACAAGAAAATCATCAGAGTGCAGGACACTTTGTGGTCACTTGGCCACTT15300.11101372216675567No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT15290.11094116417841139No Hit
AGCTGGCTCGGCTCTTGATCTCCCTGCAACCCCCGTCCCCAGGGCCGGCC15270.11079604820172281No Hit
GGAAAATCGTATTTAACAAAAAGCTGAGTGGAAATACTTACCTCTTCCTA15150.1099253523415914No Hit
AAGCAGTGAGCACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTC14990.10876442452808284No Hit
GGACAGACCCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGA14840.10767605470291858No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA14790.10731326476119715No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC14770.10716814878450859No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG14730.10687791683113144No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA14600.10593466298265573No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA14500.1052090830992129No Hit
GAGCAATGTTTCCAAGGTTGCATGTACAAGTGGAGCATTTTGAGAATTTT14440.10477373516914719No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG14270.10354024936729433No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT14270.10354024936729433No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA14210.10310490143722864No Hit
ATTTGTCTCAATTAAGAGTGGGCTCTTTAACACCATTGTTTTAAAAAAAT14200.10303234344888434No Hit
GCCGACAAAGGAAACTAGAAGCCAAGAAAGCTGCAGCTGAAAAGCTTTCC14160.10274211149550722No Hit
GTGTTACGAAAGGACTGACGATCTTCAAGCCGGGGCTTCTTTTCGGGAAA14150.10266955350716292No Hit
CACTACGTTGTTTATATAAACACTACGTTGTTTATATATTTCACTTTATA14150.10266955350716292No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT14130.10252443753047434No Hit
GAAAGCAACAATTTTATTTACTTCTTGACGGTCCAAAGGGAAACTCTAGA14010.10165374167034294No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC13980.10143606770531008No Hit
GGGGTGACTCCAGGGAGTCCACGGGCGAGAGCATGCCGGAGCTGTCCAGC13850.10049281385683438No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG13820.10027513989180152Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)