FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002434568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942756
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC58040029.875084673525652No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1310456.7453143884255145TruSeq Adapter, Index 16 (97% over 40bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT109840.5653823743177219TruSeq Adapter, Index 16 (97% over 40bp)
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA103750.5340351541830266No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC100340.5164827698383122No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG93670.48215010016697923No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA76660.3945940715148995No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG75450.38836580610225885No Hit
ACACACACACAAACACACACACACACACACACACACACACACACACACAC74270.38229196049323744No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT72270.37199730691862487TruSeq Adapter, Index 16 (97% over 40bp)
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC69010.3552170215920064No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC62590.3221711836175001No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC59810.3078616151487886No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG53100.2733230524059635No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC50570.26030031563407857No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC50180.25829285818702913No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG46860.24120373325317226No Hit
ACCCCATCTGCTTCTTTCACGCAGGCAAGTCCGCCCTGCACTGGGCCGCC45250.2329165371256092No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC41930.21582741219175233No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG41670.21448910722705272No Hit
CAAGCTCTGTGCAGGTGCCACCCTCCTGGCAGCAGAGCTCCCAGGGTTTA39700.20434887345605934No Hit
GATCGGAAGAACACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT39250.2020325764017715TruSeq Adapter, Index 16 (97% over 40bp)
ACACACACACAGACACACACACACACACACACACACACACACACACACAC37800.1945689525601774No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT35840.18448019205705707No Hit
CCACAGCTGTGCCCAGTGGGGCACTGGGGCCGCGCGTGCCCCCCTCACTG35280.18159768905616555No Hit
CCACAGCTGTGCCCAGTGGGGCATTGGGGCCGCGCGTGCCCCCCTCACTG33160.17068535626707626No Hit
GACCGACACGTGCCACCCCTTCAGGACCCAACTGCGAGATCAACCTGGAT31590.1626040532110054No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA30400.1564787343341109No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG30250.15570663531601497No Hit
CAGCCCTCACGTCTCCCCTGGCATCCCAGCCTCGCGCTCACCCTGTTGTT27880.1435074708300991No Hit
CTCTGCCTCTGCAGATGGCTTCACCCCGCTCATGATCGCCTCCTGCAGCG27670.14242653220476478No Hit
GAACAGTGCATGGGCCTATCAGGTTCAGTTTTCTCCACTGGGCCAGCTCC27150.1397499222753655No Hit
CCAACGTCTCCGACTGGTCCGAGGGCGTCTCCAGCCCTCCCACCAGCATG25660.13208040536227916No Hit
GTCCCCACGCGGAAGGCAACTGCCTGAGAGGCGCGGCGTCGCACCGCCCA25630.13192598555865998No Hit
GATTGAAGAAGACGTCCACCTCGTCTGGAGGCAGCAGCTGCGCGGGTTCC25610.13182303902291384No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC25530.13141125287992933No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC25410.13079357366545258No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA25220.1298155815758644No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT23380.12034450028722084No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA23040.1185944091795367No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA22930.11802820323293302No Hit
GTCTGAGGCCAGCAAGAAGAAGCGGCGGGAGCCCCTCGGCGAGGACTCCG22420.1154030665714068No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT22240.11447654774969168No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT22050.11349855566010349No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG21270.10948364076600457No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC21010.10814533580130496No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC20860.10737323678320901No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA20390.10495399319317504No Hit
CGCAGTGCCCGTCGCTGAAGTGGTCCTTGCAGTACTGGTCGTACAGGGGG19910.10248327633526803No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC19870.10227738326377578No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG19720.10150528424567985No Hit
CACCGGGCCCTGGCCAGCCTCACCTTGCCAGCCCGTGGGGCAGACACAGG19700.10140233770993372No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT19570.10073318522758391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)