FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002434572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3767954
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC58466415.516749939091612No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2005415.322278350531881TruSeq Adapter, Index 1 (100% over 50bp)
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG318770.8460028970629684No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA280020.743161938813478No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC191110.5071983362854218No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA191090.5071452570811639No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC175070.46462881447066495No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC173200.45966590887256054No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC160850.4268895002433682No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC155440.41253157549163283No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT155440.41253157549163283No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG154540.4101430113000318No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT150430.39923523482505363No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC150390.399129076416538No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC149940.3979347943207374No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG143950.3820375726455259No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG143300.3803124985071474No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC141750.37619886017716775No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG128540.3411400457648899No Hit
GATCGGAAGAACACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC123830.3286398931621777TruSeq Adapter, Index 1 (98% over 50bp)
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC121350.3220580718342103No Hit
ACACACACACAAACACACACACACACACACACACACACACACACACACAC120630.3201472204809294No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT119750.31781173549358616No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC117970.31308768631464184No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA117460.3117341666060679No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA109450.29047594530081844No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC109250.28994515325824044TruSeq Adapter, Index 1 (98% over 50bp)
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC109220.2898655344518537No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT108460.28784852469005723No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC103590.2749237384532826No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG100130.2657410361166829No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT99460.2639628827740466No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC89460.2374232806451459No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG84800.22505582605307817No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT84420.2240473211721799No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG82150.21802283148891943No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT81960.21751857904847033No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT81910.21738588103782583No Hit
GCCACGCCACTCACCTGTGGCTGGCTTCCGGAAGCCTCTCAGGAGCGGGG79470.21091021811837407No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA79350.21059174289282723No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA78440.20817663909909728No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA78400.2080704806905817No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG77950.20687619859478112No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA74550.1978527338709549No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA74460.19761387745179476No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA72680.19288982827285048No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG71290.18920082357693327No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA71080.18864349193222635No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG70800.1879003830726171No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG70600.1873695910300391No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA70160.18620184853636748No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG69430.18426445758095775No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC68310.18129202214252085No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC67480.1790892351658221No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA67370.1787972995424042No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT65600.17409978996558875No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC64940.1723481762250813No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT64600.17144582975269868No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT64450.17104773572076518No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG63980.16980037442070683No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG63850.16945535959303112No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA63400.1682610774972306No Hit
GATTTCTCTAATAGCTGCCACATTAGGACCTGCTCCTAGATGGGTATAAA63390.16823453789510168No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA63380.1682079982929728No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC62170.1649967064353758No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC61860.1641739787693799No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG60810.16138732054584531No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG60780.1613077017394586No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT60650.1609626869117829No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT59930.15905183555850202No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG59910.15899875635424424No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA59280.1573267614201235No Hit
GATTGAAGAAGACGTCCACCTCGTCTGGAGGCAGCAGCTGCGCGGGTTCC59200.1571144446030923No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT58640.15562822688387384No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT58230.15454010319658892No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA58140.15430124677742882No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC57920.153717375530593No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG57920.153717375530593No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG57740.1532396626922728No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT57000.15127573213473414No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA56820.15079801929641393No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA56750.1506122420815116No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC55630.14763980664307472No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC55360.1469232373855944No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC55250.1466313017621765No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT55030.1460474305153407No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC54880.14564933648340717No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG54690.14514508404295806No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT54520.14469391080676675No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC54260.14400388115141532No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA53620.1423053466151657No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC53530.1420664901960056No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT53010.14068643088530275No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG52730.1399433220256935No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC52180.138483643908604TruSeq Adapter, Index 1 (98% over 50bp)
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG52130.1383509458979595No Hit
GACAGATAACGTACCTCTTCCACTTCGACCGACAAACCTGAGGTCATTAA51930.13782015385538146No Hit
GTCCCCACGCGGAAGGCAACTGCCTGAGAGGCGCGGCGTCGCACCGCCCA51780.13742205982344796No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT51720.13726282221067454No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC50980.13529889165313588No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA50940.13519273324462028No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG50760.13471502040630007No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT50630.13437000557862436No Hit
AAGCAGTGAGCACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTC49410.1311321741188985No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG49300.13084023849548057No Hit
GTCCTCTTCACCCTCACATTGCTCCCCAGGTGGCAGTTTTGGCACAGGAA49280.13078715929122278No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA48600.12898246634645752No Hit
GAACAGTGCATGGGCCTATCAGGTTCAGTTTTCTCCACTGGGCCAGCTCC47320.12558539727395823No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG47020.12478920921009119No Hit
ATTTGTCTCAATTAAGAGTGGGCTCTTTAACACCATTGTTTTAAAAAAAT46300.12287835785681035No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA45990.12205563019081443No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT44920.11921589276302205No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA44760.11879125912895964No Hit
ATCCTCCGTGGTCATGCTCCAATAAATTCACTGCTTTGTGGCGCGACCCT44000.11677424936716319No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC43650.11584536329265166No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC43610.11573920488413605No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT43480.11539419005646034No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT43200.11465108119685112No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT43050.11425298716491762No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT42980.1140672099500153No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA42760.11348333870317949No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG42280.11220943780099225No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG42260.11215635859673447No Hit
AGTTCAGTGCAAAACTTAATTTTAATATTAAAATTTTTCTCATGCCCTAT42140.11183788337118766No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG41940.11130709132860964No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG41890.11117439331796512No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG41850.11106823490944953No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATCCCAAATGC41630.11048436366261372No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC41440.10998011122216461No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA41340.1097147152008756No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG41330.10968817559874669No Hit
GTCTCCCTGAAAGAGTCCACTTGGAAAGATTCCTTTTAGTTCTGCCAGCA40040.1062645669241185No Hit
GAAATTAAGAAAGTCTGTGTTATCTTTTTATAGATATTGAAGCACAGATT39980.1061053293113451No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC39120.10382292352825963No Hit
ATACAAAGAGCTAAATAGGCGAATTGATCTAAAACAGATACATTCAAATT39110.10379638392613075No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC39030.10358406710909952No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC38770.1028940374537481No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA38530.1022570870026545No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG38400.10191207217497879No Hit
ACTTACATACAGTTTCTTGCAGCCAAGTCTCTGTGGACAAACTTTTTGCT38330.10172629496007647No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC38150.10124858212175628No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT38040.10095664649833835No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG37970.10077086928343607No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG37960.10074432968130716No Hit
AAACTTTCTCAAATAAATTCATTTCCATTTTAATGTTATTTATTCCCAGG37790.10029315644511584No Hit
ATCCAGGTTTCTGTTGTTACAGTCTCTCTTCTGCCTCCTAGGCCGTTGGC37720.10010737923021353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)