FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002434634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274916
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC34213026.83549347564859No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC18401014.433107749843913TruSeq Adapter, Index 3 (100% over 50bp)
ACACAAACACACACACACACACACACACACACACACACACACACACACAC228781.7944711651591165No Hit
ACACACACACACACACACACACACAAACACACACACACACACACACACAC171651.3463632113802007No Hit
ACACACACACACACACACAAACACACACACACACACACACACACACACAC170161.3346761669004075No Hit
ACACACACACACACAAACACACACACACACACACACACACACACACACAC151781.1905098061362474No Hit
ACACACACACAAACACACACACACACACACACACACACACACACACACAC138161.083679238475319No Hit
ACACACACACACACACACACACACACACAAACACACACACACACACACAC131521.0315973758271133No Hit
ACACACACACACACACACACACACACACACACACACACACACACAAACAC125630.9853982536888705No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAA121230.9508861760304209No Hit
ACACACACACACACACACACACAAACACACACACACACACACACACACAC120300.9435915777980667No Hit
ACACACACACACACACACACACACACACACACACACACACACAAACACAC112910.8856269746398979No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAAAC104530.8198971540085778No Hit
GATCGGAAGAACACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC99070.7770708030960471TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGAACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC60970.4782275851899262TruSeq Adapter, Index 3 (98% over 50bp)
ACACACACACACAAACACACACACACACACACACACACACACACACACAC57970.4546966231500742No Hit
ACACACACACACACGCACACACACACACACACACACACACACACACACAC49030.38457435627131514No Hit
ACACACACACACACCCACACACACACACACACACACACACACACACACAC47600.37335793103231896No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC36820.28880334076911734TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGATCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC34160.2679392210937819TruSeq Adapter, Index 3 (98% over 50bp)
ACACACACACACACACAAACACACACACACACACACACACACACACACAC31570.24762415719937628No Hit
GATCGGAAGAGCACGCGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC30160.2365646050406458TruSeq Adapter, Index 3 (98% over 50bp)
ACACACACACACACTCACACACACACACACACACACACACACACACACAC28840.22621098174311094No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG22680.1778940730212814No Hit
ACACACACACACACACACAAACACAAACACACACACACACACACACACAC21430.16808950550467638No Hit
GATCGGAAGAGCACAAGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC21370.16761888626387933TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACTTAGGCATCTCGTATGC20530.1610302168927208TruSeq Adapter, Index 3 (98% over 50bp)
ACACAAACACACACACACACACACAAACACACACACACACACACACACAC18610.14597040118721546No Hit
GATCGGAAGAGCAAACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC18200.142754503041769TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAAATCCAGTCACTTAGGCATCTCGTATGC17550.13765612793313442TruSeq Adapter, Index 3 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC17460.13695019907193887TruSeq Adapter, Index 3 (98% over 50bp)
ACACAAACACAAACACACACACACACACACACACACACACACACACACAC16960.13302837206529686No Hit
GATCGGAAGAGCACTCGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC16790.1316949508830386TruSeq Adapter, Index 3 (98% over 50bp)
ACACAAACACACACACACAAACACACACACACACACACACACACACACAC16740.13130276818237438No Hit
ACACACACACACACACACACACACAAACAAACACACACACACACACACAC16710.13106745856197585No Hit
ACACACACACACACACACACACACACACACACACACAAACACACACACAC16150.12667501231453682No Hit
ACACAAACACACACAAACACACACACACACACACACACACACACACACAC16040.12581221037307555No Hit
ACACACACACACACACACACACACACACACACACACACACAAACACACAC14990.11757637365912735No Hit
ACACACACACAAACACACACACACACACAAACACACACACACACACACAC14670.11506640437487646No Hit
ACACAAACACACACACACACACACACACAAACACACACACACACACACAC14350.11255643509062557No Hit
ACACAAACACACACACACACACACACACACACACACACACACACAAACAC14090.11051708504717173No Hit
ACACACACACAAACACACACACACAAACACACACACACACACACACACAC14020.10996802926624186No Hit
ACACACACACACACAAACAAACACACACACACACACACACACACACACAC13760.10792867922278801No Hit
ACACACACACACACACACACACACACACACACACAAACACACACACACAC13340.10463434453720873No Hit
ACACAAACACACACACACACACAAACACACACACACACACACACACACAC13310.1043990349168102No Hit
ACACACACACAAACACACAAACACACACACACACACACACACACACACAC13210.1036146695154818No Hit
ACACACACACACACAAACACACACAAACACACACACACACACACACACAC13210.1036146695154818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)