FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002434635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274916
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT56206144.08612018360426No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG14878211.66994531404422Illumina Single End PCR Primer 1 (100% over 50bp)
ACACACACACACACACACACACACACACACACACACACACACACACACAC101160.7934640399838107No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG99420.7798160820006965Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG53190.41720395696657664No Hit
GGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT41870.3284137935362016No Hit
GATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG33120.2597818209199665Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGAGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG27420.21507299304424762Illumina Single End PCR Primer 1 (98% over 50bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAT26150.20511155244737692No Hit
GTGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT23430.1837768135312444No Hit
GTGTGTGTGTGTGTGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT22000.1725603882922483No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCCG18020.1413426453193779Illumina Single End PCR Primer 1 (98% over 50bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGT17960.14087202607858085No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGGTGTGTGTGTGTGT17950.14079358953844803No Hit
GAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC17690.13875423949499419Illumina Single End PCR Primer 1 (100% over 48bp)
GTGTGTGTGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT17380.13632270675087615No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGT16950.13294993552516401No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG16920.1327146259047655No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG16650.1305968393211788Illumina Single End PCR Primer 1 (98% over 50bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGGTGTGTGTGTGT15720.12330224108882466No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGGGTGTGTGTGTGTGTGTGTGTGTGT15030.11789011981965872No Hit
GTGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT14670.11506640437487646No Hit
GTGTGTGTGTGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT14330.11239956201035989No Hit
GTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT13760.10792867922278801No Hit
GTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT13410.10518340031813861No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGAT13220.10369310605561466No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTGTGTGTGTGTGTGTGTGT12920.10134000985162943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)