FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002434739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721838
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT29703617.251100277726476No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA165470.9610079461598593No Hit
GGGGCCACACTTACTCTGCACGGCCTCGATCTTGTAGGGGATGTTGAGGC145230.843459140755402No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG107360.623519750406252Illumina Single End PCR Primer 1 (100% over 50bp)
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA104820.6087680722576688No Hit
GGAGAGCCGAGCCAGGCAGACGTGCAGCCACTGGGCCCCAGCAGCCTGGC86950.504983627960354No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC86410.5018474444169544No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC86200.5006278174834101No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC85840.49853702845447717No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT82210.477454905746069No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC79160.4597412764731641No Hit
GGAGGGGTGACCCAAACACTGATTCTGCTCAGACATCGTCGTGCACAAGC76380.4435957389719591No Hit
GCTGCTCCATGGTCTCAATGATGCTCTTTTGCTGCACATTATCACAGCTT75180.4366264422088489No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC71730.4165897140149073No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT69550.4039288248952572No Hit
GTGTGCCTCCCACAGCATGACCTACCATCATTGGAAAGCAGTTTGTAGTC69240.4021284232314538No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT68990.4006764864058059No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT66940.3887706044354928No Hit
GGGGAGGGTCCCCACGCCTGGCCTGGCCCCCCTCACCGGCCCCCGCCCTC65330.37942013127832No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC57050.3313319836128602No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG53700.3118760301491778No Hit
GGACAGGAAGAGAATTAACCAACCGGCACTCACTTCCATTTTCATTGCCC51230.29753089431177615No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA47680.27691339138757537No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT45650.2651236643633141No Hit
GTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTG45310.26314903028043285No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC43950.2552504939489081No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA43750.2540889444883897No Hit
GCTGTGCCTTCAGGGTTCTCGGGCTGCCACGCCGATGGCGAGAGCGGCAG43430.25223046535156035No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA43360.251823923040379No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG42880.24903620433513488No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT42130.24468039385819104No Hit
GGGACCCGAGCTGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA42090.2444480839660874No Hit
GGGACCCGAGCCGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA38660.22452751071819765No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC38160.22162363706690175No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG38090.22121709475572035No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT37800.21953284803796874No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA37280.216512819440621No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC36800.21372510073537695No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT36220.21035660729987377No Hit
GGTAGCCTTAGAAAGCTGGGCTGACCTAAGGGCAGAGTTGGTGTTCTGCA35820.20803350837883702No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT35380.20547809956569665No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG35010.2033292330637377No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA34310.19926380995192344No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA33740.19595339398944617No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA33480.1944433796907723No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA33020.1917718159315801No Hit
GAGCCGCCGCCGCCGGCCATGGAGGTGGCGCCCGAGCAGCCGCGCTGGAT32740.1901456466868544No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA32490.18869370986120645No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG31860.18503482906057364No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA31230.18137594825994083No Hit
AGGCCACCAACACAAATCTGAATACTGAGAGGAGAAAGATACACACACAC31170.18102748342178532No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT31110.1806790185836298No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT30830.1790528493389041No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC30830.1790528493389041No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT30110.17487127128103805No Hit
GGAAGACACCTTGTGCGTCGGCAGACACAGCCGCATGCAGCGGGGTGCGG30070.17463896138893437No Hit
GGAAAATGGCTGCTGCACCCCCAAGGAGGGCCGAGGAGCCCCTGCAGAAG29850.17336125698236418No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC29320.17028315091199056No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG29210.16964429870870548No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC28910.16790197451792793No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT28760.16703081242253917No Hit
GAGGGAGGTCATACCCTCCACATCGGGAGGGGGGCGGCCGTAGCTCATAG28390.16488194592058023No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA28300.16435924866334695No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA28140.16343000909493227No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT28060.16296538931072493No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT27660.16064229038968822No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG27600.1602938255515327No Hit
GGACAAGAACAGCAACGAGTACCGGGTGCGGCGCGAGCGCAACAACATCG27490.15965497334824763No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG27490.15965497334824763No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG27080.157273796954185No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT26980.15669302222392584No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC26840.155879937601563No Hit
GGTGTTTAGGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCAC26490.15384722604565587No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT26460.15367299362657813No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT26460.15367299362657813No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG26360.15309221889631894No Hit
GTAGTTGGGGGTTAAGCTTTGTGGATGTAGCCTTTATATTTAGTATAATT26350.15303414142329302No Hit
GCTGCTCTTCCTCCTCTCCCCTACGTCTCTTGCTCCTAGATCTTCACGCT25610.1487364084193751No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC25500.14809755621609No Hit
GAGGATGCTCGGCCAGGTCCCACCTCCCACCGGGGACCCAGAAGCAGGGG25380.147400626539779No Hit
GGAGTTTTAGAAGAACACCACCACTACCCCAACCAAAGTAACACAACACT25130.14594868971413105No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG24970.14501945014571638No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG24700.14345135837401662No Hit
GGGCGGCTACTCCTGCAAGGTGGGGGTCCCTCCTAGGGTAAGGGTTGTGG24650.14316097100888703No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT24590.14281250617073152No Hit
GGGTCCTGCCCTTGCACAGATGGGGTCTCATTCTGCCCCATCCCCATAGG24560.14263827375165375No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGCGGGGGG24360.1414767242911354No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACTGCATCTT23700.13764361107142484No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC23670.1374693786523471No Hit
GGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAAGCCAAGCCAA23650.13735322370629524No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG23610.1371209138141916No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT23500.13648206161090648No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT23420.13601744182669914No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC23150.13444935005499936No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA23040.13381049785171426No Hit
AGGTACTCACAAGCCTTCCCTGAAGCCATCAATCAGTGCTTGGAAGAGAG22960.13334587806750695No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT22900.13299741322935144No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG22820.13253279344514407No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG22680.13171970882278125No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC22440.1303258494701592No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG22340.12974507473990005Illumina Single End PCR Primer 1 (98% over 50bp)
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA22160.12869968022543352No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA22150.1286416027524076No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG21940.12742197581886333No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG21870.1270154335076819No Hit
AGGTGACATGTATTTTGCTCTGTTCAATTTGAGTTATGTCTGTTTTTAAG21640.1256796516280858No Hit
GGCCACACTCGTTGACATCCTGCCGGCAGGTGGGGCCATGGAAGCTGGGT21290.12364694007217869No Hit
GGTCGGCCCTGGCATCCACAGAGCGCACACAGACGCCCGAAGGCTTGGGA21250.12341463018007502No Hit
GCAGGAGATGATCCGGCAACTGGAACAGACAAACTATCGACTGAAGTTGT21190.12306616534191951No Hit
GGACAGAGGAATTGAGAGGTCCTTCTGGGACTCTTTGAGCTTCTCAGCCA21180.1230080878688936No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG21160.12289193292284176No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT21100.12254346808468625No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA20980.12184653840837524No Hit
GAGCACAGTGCAGTCAGCCCCCACGTGCAGGGCCGCTCACCTGTGTAGCC20790.1207430664208828No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT20770.12062691147483097No Hit
GTCTCCCTGAAAGAGTCCACTTGGAAAGATTCCTTTTAGTTCTGCCAGCA20480.11894266475707936No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC20470.11888458728405345No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG20370.11830381255379427No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA20360.11824573508076835No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG20180.11720034056630181No Hit
GAGATCACTCACCCATCGCATACCTCAGGGCAGATCAATTCCGCACAGAC20180.11720034056630181No Hit
AGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACACCCAGCT20170.1171422630932759No Hit
GAGAATCACAATCACTGCAGCCTCACAGGTGTGGCCAGCTCGCTCCCGCA20020.11627110099788714No Hit
GTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGCTGA19940.1158064812136798No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC19890.11551609384855022No Hit
GCAGCAATTGATGTCACGTATCCAGCAAATCTGCTGTCACTTCCACCATA19590.1137737696577727No Hit
GAGAGACCAATACATGAGGACAGGCGAAGGCTTCCTCTGTGTATTTGCCA19570.11365761471172084No Hit
CGAGACAAGGAGCAAACACGAGATCTTGTGCCCCCAACACAGCACTATCT19520.11336722734659126No Hit
GTGTCTGTGTCCAAAGCAAGCTATCTTCTTAGGGAAGGGGAAGAATTCAC19430.112844530089358No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC19360.11243798777817657No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA19350.11237991030515065No Hit
GAGAAATCAATGTAAACACCATCTTACCAATGGGATTGGTGGACGTCTCC19110.11098605095252864No Hit
GGTTATCATGTGAAACAAAACTCACCTGGGTCACTTTGATATGGTCTTGG19020.11046335369529538No Hit
GAAGAGGACGAAAAACGATTTCTTGTTACTGTTATAAAGGTATGCAAGGG19000.11034719874924355No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA18930.10994065643806213No Hit
GGTTATAATGTGAAACAAAACTCACCTGGGTCACTTTGATATGGTCTTGG18870.1095921915999066No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT18800.10918564928872518No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA18720.10872102950451785No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT18660.10837256466636234No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC18640.1082564097203105No Hit
ATCCAGGTTTCTGTTGTTACAGTCTCTCTTCTGCCTCCTAGGCCGTTGGC18560.10779178993610317No Hit
GGTATTGGTCAGAAGTTCAACACTGTAGCCATTGCAACATGCTCAGTTAA18450.10715293773281807No Hit
GGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTTCATTCAAGG18430.10703678278676622No Hit
GCACAGCAGCCAGTAGAATTCCCCTTTTTATTTTTTTTTCCCCGCAGACC18340.10651408552953298No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG18150.10541061354204054No Hit
GGTGTTTCTGATGTACCAACCTCACCAACATTACAGAGGCCTACTCATAT18090.10506214870388503No Hit
AGGTACAGCTCCAGAGAGTAGAACAGGGCAGGCACAGGGCCCTTACCCTG18070.1049459937578332No Hit
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA18020.1046556063927036No Hit
GCAAGGGCAGGACCCAGAGCCTGTCCTGGGCAGCTCCGGCCTCTCCCTCC18010.10459752891967769No Hit
GGATTCTGTTGTTAGCTGATAAGAGTAACACAGCCAGAGCTGATTCAGAC17950.1042490640815222No Hit
GGATAGAGATGAAGATCTTAATTATAGCTATGCAGCATAGATTTAGTCAA17820.10349405693218525No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT17250.10018364096970796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)