FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002434743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546415
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT42787916.80319193847036No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG401001.574762951050791Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGCCACACTTACTCTGCACGGCCTCGATCTTGTAGGGGATGTTGAGGC240720.9453290213888937No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA217020.8522569966011039No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA158050.6206765197346074No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC128820.5058876891629998No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC119760.47030825690235095No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC118940.46708804338648646No Hit
GGAGAGCCGAGCCAGGCAGACGTGCAGCCACTGGGCCCCAGCAGCCTGGC116070.4558172960809609No Hit
GGGACCCGAGCTGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA113210.4445858196719702No Hit
GCTGCTCCATGGTCTCAATGATGCTCTTTTGCTGCACATTATCACAGCTT105150.4129334770648146No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC104770.4114411829964872No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT98420.3865041636968051No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT97980.3847762442492681No Hit
GGAGGGGTGACCCAAACACTGATTCTGCTCAGACATCGTCGTGCACAAGC93280.36631892287784984No Hit
GTGTGCCTCCCACAGCATGACCTACCATCATTGGAAAGCAGTTTGTAGTC92260.36231329143128677No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT86390.3392612751652814No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC85880.33725845944199984No Hit
GGGGAGGGTCCCCACGCCTGGCCTGGCCCCCCTCACCGGCCCCCGCCCTC85480.3356876235806025No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT85430.33549126909792787No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA82350.3233958329651687No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA77330.30368184290463257No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC72320.2840071237406314No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT71780.28188649532774507No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT69880.2744250249861079No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG69860.27434648319303806No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT68060.2672777218167502No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA67930.26676720016179606No Hit
GGACAGGAAGAGAATTAACCAACCGGCACTCACTTCCATTTTCATTGCCC67010.2631542776805823No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT66310.26040531492313707No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC66310.26040531492313707No Hit
GGTGTTTCTGATGTACCAACCTCACCAACATTACAGAGGCCTACTCATAT66090.2595413551993685No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT64930.2549859312013164No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC56900.22345140128376562No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA56130.22042754225057581No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA55990.21987774969908674No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT55190.21673607797629216No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA54260.21308388459854344No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA51800.20342324405095008No Hit
GCTGTGCCTTCAGGGTTCTCGGGCTGCCACGCCGATGGCGAGAGCGGCAG51760.2032661604648103No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC51080.2005957395004349No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT50770.199378341707852No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT49240.19336989453800735No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATCCCAAATGC48920.1921132258488895No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC47880.18802905260925656No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG47380.1860655077825099No Hit
GTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTG47040.18473029730032223No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT45950.1804497695780146No Hit
GGTAGCCTTAGAAAGCTGGGCTGACCTAAGGGCAGAGTTGGTGTTCTGCA45520.17876112102701247No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG45440.17844695385473303No Hit
GGTTATCATGTGAAACAAAACTCACCTGGGTCACTTTGATATGGTCTTGG43740.17177090144379453No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA42500.1669013102734629No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA42420.16658714310118342No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA41880.1644665146882971No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG41540.16313130420610936Illumina Single End PCR Primer 1 (98% over 50bp)
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG41390.16254224075808538No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC41200.16179609372392167No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA41060.1612463011724326No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA40510.15908640186301132No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA40260.15810462944963802No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG39850.1564945226917058No Hit
GTAGTTGGGGGTTAAGCTTTGTGGATGTAGCCTTTATATTTAGTATAATT39280.15425608158921464No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG37850.1486403433847193No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA37550.14746221648867133No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT37110.1457342970411343No Hit
GAGGGAGGTCATACCCTCCACATCGGGAGGGGGGCGGCCGTAGCTCATAG36810.14455617014508632No Hit
GGAAGACACCTTGTGCGTCGGCAGACACAGCCGCATGCAGCGGGGTGCGG36230.14227845814606024No Hit
GCTGCTCTTCCTCCTCTCCCCTACGTCTCTTGCTCCTAGATCTTCACGCT35860.14082543497426775No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA35810.1406290804915931No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG35630.1399222043539643No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG35620.13988293345742936No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA35570.1396865789747547No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG35520.13949022449208004No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC35440.1391760573198006No Hit
GGGCGGCTACTCCTGCAAGGTGGGGGTCCCTCCTAGGGTAAGGGTTGTGG35350.1388226192509862No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG34910.13709469980344918No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT34670.1361521982866108No Hit
GCGGAAGCACCAGCTGAGAAGGTGGAGGGGACAGGATGGTACCTACCGTA33790.13269635939153673No Hit
GAGGATGCTCGGCCAGGTCCCACCTCCCACCGGGGACCCAGAAGCAGGGG33780.1326570884950018No Hit
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA33630.1320680250469778No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA33590.13191094146083807No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG33380.1310862526336045No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG33180.13030083470290585No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG32960.12943687497913733No Hit
GGAAAATGGCTGCTGCACCCCCAAGGAGGGCCGAGGAGCCCCTGCAGAAG32850.12900489511725308No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT32840.12896562422071817No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC32550.1278267682212051No Hit
GGACAAGAACAGCAACGAGTACCGGGTGCGGCGCGAGCGCAACAACATCG32500.12763041373853046No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC32440.12739478835932086No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT32430.12735551746278592No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT32350.12704135029050645No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG31940.12543124353257423No Hit
GGTGTTTAGGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCAC31760.12472436739494545No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC31710.12452801291227078No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT31620.1241745748434564No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT31510.12374259498157214No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT31190.12248592629245428No Hit
GAAGAGGACGAAAAACGATTTCTTGTTACTGTTATAAAGGTATGCAAGGG31080.12205394643057003No Hit
GGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAAGCCAAGCCAA31050.12193613374096525No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC30830.12107217401719672No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG30690.12052238146570766No Hit
AGGCCACCAACACAAATCTGAATACTGAGAGGAGAAAGATACACACACAC30300.11899081650084531No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGCGGGGGG30160.11844102394935624No Hit
AGGTACTCACAAGCCTTCCCTGAAGCCATCAATCAGTGCTTGGAAGAGAG29780.11694872988102882No Hit
GCAGCAATTGATGTCACGTATCCAGCAAATCTGCTGTCACTTCCACCATA29640.11639893732953976No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG29560.11608477015726029No Hit
GGGTGCAGGAGGGCCACAGTCCCTGGGTGAGGTCACACAGCTCAGGCCTG29160.11451393429586301No Hit
GGACAGAGGAATTGAGAGGTCCTTCTGGGACTCTTTGAGCTTCTCAGCCA29000.1138855999513041No Hit
GAGCACAGTGCAGTCAGCCCCCACGTGCAGGGCCGCTCACCTGTGTAGCC28760.1129430984344657No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT28430.11164715884881295No Hit
GGGTCCTGCCCTTGCACAGATGGGGTCTCATTCTGCCCCATCCCCATAGG28350.11133299167653347No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG28170.1106261155389047No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG28140.1105083028492999No Hit
GGATTCACATTAACTCTATGACCATATTTTATTCCAGACACTTCTGAGAA27860.10940871774632178No Hit
GTTGTTTTAAAAGGACAAAAAAGACAGTAATGAGTATCTAATGACTTACA27750.10897673788443753No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT27650.1085840289190882No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT27230.10693465126462105No Hit
GGAACATTCTCCAACACTTCCACATGCAATTCTTCTCCAGTAAGCCAGGA27130.10654194229927172No Hit
GCACAGCAGCCAGTAGAATTCCCCTTTTTATTTTTTTTTCCCCGCAGACC27060.1062670460235272No Hit
GTCTCCCTGAAAGAGTCCACTTGGAAAGATTCCTTTTAGTTCTGCCAGCA27040.10618850423045734No Hit
GGACAGACAGATGGACAGACAGGCAGGCAGACAGGCAGACAGACAGGCAG27030.10614923333392239No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA26650.10465693926559497No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT26630.1045783974725251No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT26590.10442131388638538No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC26540.10422495940371071No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG26520.10414641761064085No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT26390.10363589595568672No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA26220.10296829071459287No Hit
AGGTGACATGTATTTTGCTCTGTTCAATTTGAGTTATGTCTGTTTTTAAG26210.10292901981805794No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC26120.10257558174924354No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA25800.1013189130601257No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG25540.10029786975021746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)