FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002434782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762511
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC25943534.02377146034615No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10305013.514559134228884TruSeq Adapter, Index 9 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC95541.2529655309890613TruSeq Adapter, Index 9 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC53560.7024160962923813TruSeq Adapter, Index 9 (98% over 50bp)
ACACACACACAAACACACACACACACACACACACACACACACACACACAC30580.401043394783813No Hit
GATCGGAAGAACACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC28570.3746831193254917TruSeq Adapter, Index 9 (98% over 50bp)
ACACACACACACACACACACACACACACACACACACACACACACACACAG23600.3095037317494436No Hit
ACACACACACAGACACACACACACACACACACACACACACACACACACAC14250.18688254989108352No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT11100.1455716704414756No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT10990.14412906830196548No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA9900.12983419255591067No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC9550.12524409483928756No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC9480.12432607529596294No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA9280.12170316231503546No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG9210.12078514277171083No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG9180.12039170582457172No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA9060.11881795803601522No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG8870.1163261907041341No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA8760.114883588564624No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC8710.11422786031939211No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT8630.11317869512702111No Hit
GATCGGAAGACCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC8510.11160494733846461TruSeq Adapter, Index 9 (98% over 50bp)
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC8130.10662141267470239No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG8050.10557224748233139No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC7910.10373620839568215No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA7860.1030804801504503No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG7800.10229360625617205No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC7750.10163787801094017No Hit
ACACACACACACACACACACACACACACACACACACACACACACAGATCG7680.10071985846761555No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG7630.10006413022238367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)