FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002434832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590545
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC34499213.317352140186717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT2061377.957283119961243TruSeq Adapter, Index 7 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT183900.7098892318025743TruSeq Adapter, Index 9 (97% over 35bp)
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA106820.4123456647153398No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT106480.4110331995776951TruSeq Adapter, Index 9 (97% over 35bp)
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA95650.36922732475212744No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG88160.3403144898081292No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC80080.3091241418311591No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA79640.3074256575353835No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT78500.30302503913269213No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG77350.2985858188141878No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG75950.2931815506003563No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG73630.28422590613172133No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT73230.28268182949919807No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC69480.2682061110692924No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC69360.2677428880795354No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG68020.26257023136058244No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC66860.25809240912626497No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC66780.2577835937997603No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA65560.2530741600705643No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG64720.2498315991422654No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC64430.24871214358368607No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC63970.24693645545628432No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC63910.24670484396140582No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC63850.24647323246652733No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG63130.24369389452798546No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG61270.2365139381867522No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT60120.2320747178682478No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT59760.23068504889897684No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG59630.2301832239934068No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA58090.22423852895819218No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC56900.21964490097643546No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG56230.217058572616959No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA55870.21566890364768804No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT55220.21315977911983772No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG54890.21188591589800604No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC53780.20760110324275394No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT53690.20725368600043617No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG53170.20524638637815593No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC52100.20111598138615622No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG50850.19629074190952098No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC50460.19478526719281078No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC49970.19289377331796975No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT49410.1907320660324372No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT49280.19023024112686712No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTCC48700.18799133000970839No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA48380.18675606870368977No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG48370.18671746678787668No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG48010.18532779781860573No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC47980.18521199207116648No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG47910.1849417786604749No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT47840.18467156524978334No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA47450.18316609053307314No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT46540.17965331619408273No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT46510.17953751044664348No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC46150.17814784147737253No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG46030.17768461848761555No Hit
ATCCAGGTTTCTGTTGTTACAGTCTCTCTTCTGCCTCCTAGGCCGTTGGC46030.17768461848761555No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA45980.17749160890855012No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA45730.1765265610132231No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA45340.1750210862965129No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC45210.17451926139094284No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT45160.17432625181187741No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA44390.17135390429427014No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC43930.16957821616686838No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG43260.16699188780739188No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC43220.16683748014413954No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA41340.15958031997128017No Hit
CTCCCTGCCCCCCAGCAGAGGTTCTAGACGCTGGAGCTGACCTTGGCTAT40540.15649216670623362No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC40150.15498669198952345No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC39840.15379003259931792No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA39770.15351981918862634No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT39310.15174413106122459No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT39210.15135811190309373No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG39110.15097209274496293No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA39100.15093349082914984No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG39050.15074048125008443No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT38490.14857877396455185No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG38470.1485015701329257No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC37850.14610825135251462No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT37690.14549062069950533No Hit
ATCCTCCGTGGTCATGCTCCAATAAATTCACTGCTTTGTGGCGCGACCCT37560.14498879579393525No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA37320.1440623498144213No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA37210.1436377287404774No Hit
AAACTTTCTCAAATAAATTCATTTCCATTTTAATGTTATTTATTCCCAGG36920.142518273181898No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT36900.14244106935027184No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG36710.1417076329498233No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA36300.14012495440148695No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG36250.13993194482242152No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA35990.1389282950112814No Hit
CTTCACAGGTGCTTTCAAGAACAGGAGCAGAAGTCACAACAAGCTTCAGT35690.13777023753688897No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC35440.1368051896415619No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA35370.13653497623087035No Hit
ATACAAAGAGCTAAATAGGCGAATTGATCTAAAACAGATACATTCAAATT35320.13634196665180492No Hit
CAACACCTACGAAGGAAACATCATGAAGAATAAAGAGAAACTGCAGAGCC35030.13522251109322556No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA34860.13456627852440317No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG34810.13437326894533777No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT34680.1338714440397677No Hit
CCACAGCTGTGCCCAGTGGGGCACTGGGGCCGCGCGTGCCCCCCTCACTG34580.13348542488163687No Hit
AAACCTCTCATAATGAAGGCCCCCGCTGTGCTTGCACCTGGCATCCTCGT34540.13333101721838453No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA34480.13309940572350604No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC34290.1323659693230575No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG34220.13209575591236591No Hit
GTCCTCTTCACCCTCACATTGCTCCCCAGGTGGCAGTTTTGGCACAGGAA33900.1308604946063473No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG33800.1304744754482165No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG33710.13012705820589876No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA33660.12993404862683336No Hit
CATTTCTTTCATCCAGCCATGTTATCCCTTATTTGATCCACTCGAAGAGC33600.12970243713195487No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA33560.12954802946870253No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT33370.128814593068254No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC33280.12846717582593625No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT33250.128351370078497No Hit
AAAACAAAATGAAATGAATGGTGCTTACTTCAAGCAAAGCTCAGTGTTCA33030.1275021279306092No Hit
AACCTCATTTCCCCATAAAGATTCAGAACACAAAGATCATCCTTTCAGAG32980.1273091183515438No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC32860.1268458953617868No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT32830.12673008961434756No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT32660.12607385704552518No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC32620.12591944938227284No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA32490.12541762447670277No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT32320.12476139190788038No Hit
GTATCAAGTACCTTTGCTGAGCAAGTTGCGCAGCTTGTTGACCAGACATA31940.12329451910698327No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC31940.12329451910698327No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT31850.12294710186466554No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG31210.12047657925262831No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG30880.11920271603079662No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA30740.11866228920941346No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT30730.11862368729360039No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT30490.11769724131408643No Hit
CACCTGACTGTTTTCTCTTTTCCACGTTGAGTACACAGAACTAGACACAT30400.1173498240717687No Hit
CTTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATT30260.11680939725038553No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA30160.11642337809225471No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC29910.11545833019692767No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG29700.11464768996485294No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA29690.11460908804903987No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA28860.11140512903655408No Hit
AAACACTTCCTTCTTTGTGGATCTCTTTTTTCTCTTTTCTGTGAGAATAC28710.11082610029935785No Hit
CACAAGAAAATCATCAGAGTGCAGGACACTTTGTGGTCACTTGGCCACTT28490.10997685815147007No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT28480.10993825623565698No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG28130.10858718918219912No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA28090.1084327815189468No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA27870.10758353937105898No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT27840.10746773362361974No Hit
GTCATTGCCAATCATACGCATAAATTCCTAAAACAAACCCACTTTCAATA27790.10727472404455432No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT27780.10723612212874124No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG27740.10708171446548892No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA27340.10553763783296564No Hit
ATTACTTTTCCAAGGACGGTTGAAGAAATTGCTTGCTTCCACTAAAAATG27320.10546043400133948No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC27000.10422517269532086No Hit
AAAACTCCACCATTCAGAGTGCTTTTTCCTTCTGAAGGAAGCTGAGGAAC26940.10399356120044238No Hit
AAATTAATACTTTTAAAAGTCATTACTGCTAGGTTTTTAATGCTTTAATG26880.10376194970556389No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG26690.10302851330511534No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATCCCAAATGC26670.10295130947348917No Hit
GATTTCTCTAATAGCTGCCACATTAGGACCTGCTCCTAGATGGGTATAAA26320.10160024242003131No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC26310.10156164050421823No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC26180.10105981559864817No Hit
AGTTCAGTGCAAAACTTAATTTTAATATTAAAATTTTTCTCATGCCCTAT26150.10094400985120892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)