FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002434837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537218
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT12611223.47501386774084No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG115802.155549516211296Illumina Single End PCR Primer 1 (100% over 50bp)
ACACACACACACACACACACACACACACACACACACACACACACACACAC34470.6416389622090102No Hit
GGGGCCACACTTACTCTGCACGGCCTCGATCTTGTAGGGGATGTTGAGGC25630.4770875138212048No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA24610.4581008082379965No Hit
GCTGCTCCATGGTCTCAATGATGCTCTTTTGCTGCACATTATCACAGCTT19650.3657732987353365No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT19430.3616781269428798No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA19100.35553536925419477No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC17290.3218432740526192No Hit
GGAGGGGTGACCCAAACACTGATTCTGCTCAGACATCGTCGTGCACAAGC17070.31774810226016253No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT17050.31737581391539377No Hit
GTGTGCCTCCCACAGCATGACCTACCATCATTGGAAAGCAGTTTGTAGTC16840.31346678629532143No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC15540.2892680438853501No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG15150.2820084211623587Illumina Single End PCR Primer 1 (98% over 50bp)
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT15000.27921625857659277No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG14980.27884397023182395No Hit
GGAGAGCCGAGCCAGGCAGACGTGCAGCCACTGGGCCCCAGCAGCCTGGC14620.27214278002598574No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA14360.26730303154399143No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC13910.25892654378669366No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA13560.25241149775323984No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT13150.24477958668547964No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG11860.22076698844789266No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT11800.2196501234135863No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC11660.21704410500020477No Hit
GAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC11450.21313507738013243Illumina Single End PCR Primer 1 (100% over 48bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAT11290.21015677062198213No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT11270.20978448227721336No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC11140.2073646080362162No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT11010.20494473379521908No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA10980.2043863012780659No Hit
GGACAGGAAGAGAATTAACCAACCGGCACTCACTTCCATTTTCATTGCCC10390.19340379510738656No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC9900.1842827306605512No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA9860.1835381539710136No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC9810.18260743310909167No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGAT9710.1807459913852477No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT9420.17534781038610026No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT9260.17236950362794992No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC9090.1692050526974152No Hit
GTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTG8870.1651098809049585No Hit
GGGGAGGGTCCCCACGCCTGGCCTGGCCCCCCTCACCGGCCCCCGCCCTC8850.16473759256018972No Hit
GGTGTTTCTGATGTACCAACCTCACCAACATTACAGAGGCCTACTCATAT8730.16250386249157697No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG8580.15971169990581105No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA8550.15915326738865787No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG8360.15561652811335436No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT8310.15468580725143238No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT8300.15449966307904797No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG8220.15301050969997282No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA8150.15170750049328205No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC8030.1494737704246693No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT7860.1463093194941346No Hit
GCTGTGCCTTCAGGGTTCTCGGGCTGCCACGCCGATGGCGAGAGCGGCAG7840.14593703114936582No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA7840.14593703114936582No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT7770.14463402194267505No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG7770.14463402194267505No Hit
GGTAGCCTTAGAAAGCTGGGCTGACCTAAGGGCAGAGTTGGTGTTCTGCA7750.14426173359790626No Hit
GAATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC7740.14407558942552187Illumina Single End PCR Primer 1 (97% over 48bp)
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA7720.14370330108075308No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA7670.1427725802188311No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC7660.1425864360464467No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA7650.1424002918740623No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA7460.1388635525987588No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA7410.13793283173683682No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG7390.13756054339206802No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA7180.13365151577199572No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG7100.13216236239292056No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA7060.131417785703383No Hit
GGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGGAGCTGCACTG7040.1310454973586142No Hit
GTAGTTGGGGGTTAAGCTTTGTGGATGTAGCCTTTATATTTAGTATAATT6870.1278810464280795No Hit
AGGATCCCGGTCTTCAAAGGCACCAGAAAACCCTAGGGCACCTATCCTCA6730.12527502801469795No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGATCG6720.12508888384231356No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT6710.12490273966992915No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGGTCCCGC6710.12490273966992915No Hit
AGGCCACCAACACAAATCTGAATACTGAGAGGAGAAAGATACACACACAC6640.1235997304632384No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG6630.12341358629085399No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG6570.12229672125654763No Hit
GGGACCCGAGCCGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA6530.12155214456701005No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT6480.12062142370508806No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG6460.12024913536031927No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA6440.11987684701555049No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT6420.11950455867078169No Hit
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6410.11931841449839731Illumina Single End PCR Primer 1 (98% over 50bp)
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA6390.1189461261536285No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT6320.11764311694693774No Hit
GGATTCACATTAACTCTATGACCATATTTTATTCCAGACACTTCTGAGAA6290.11708468442978455No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT6290.11708468442978455No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC6220.1157816752230938No Hit
GGGACCCGAGCTGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA6200.11540938687832498No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT6200.11540938687832498No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA6190.1152232427059406No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC6180.1150370985335562No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA6170.11485095436117182No Hit
GGAACATTCTCCAACACTTCCACATGCAATTCTTCTCCAGTAAGCCAGGA6030.1122449359477903No Hit
GGAGTTTTAGAAGAACACCACCACTACCCCAACCAAAGTAACACAACACT6010.11187264760302149No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA6010.11187264760302149No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG5960.11094192674109953Illumina Single End PCR Primer 1 (98% over 50bp)
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC5930.11038349422394633No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG5870.10926662918963996No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT5870.10926662918963996No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG5720.10647446660387402No Hit
AAGCAGTGAGCACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTC5700.10610217825910524No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC5660.10535760156956767No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG5620.10461302488003008Illumina Single End PCR Primer 1 (98% over 50bp)
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG5620.10461302488003008No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT5540.10312387150095492No Hit
GGTGTTTAGGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCAC5520.10275158315618614No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC5490.10219315063903295No Hit
GCATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5480.10200700646664856Illumina Single End PCR Primer 1 (100% over 48bp)
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT5450.10144857394949536No Hit
GGGACACCGATGGGCCTGCATCTGACCTGTTGTGCTCACTGCTTAGGACC5380.10014556474280459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)