FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002434840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850833
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC19543622.969960027408433No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC789809.28266769154464TruSeq Adapter, Index 12 (100% over 50bp)
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG48160.5660335224421243No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA47380.5568660359906116No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC35840.42123424925925534TruSeq Adapter, Index 12 (98% over 50bp)
ACACACACACACACACACACACACACACACACACACACACACACACACAG34740.40830574272507064No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC34320.40336940386656367No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA33200.39020583357721195No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC32680.3840941759428701No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT31950.37551434887927476No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG29620.34812942140231984No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC27990.3289717253562097No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT27300.3208620258029484No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG26020.3058179454722607No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC25050.29441735334666147No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC25010.2939472258363275No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC23680.2783154861177223No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA23010.27044085031962795No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG22910.26926553154379296No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC22590.26550451146112103No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC21360.2510480905183508No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA20730.24364358223059052No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT20360.2392949027600011No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC19610.23048001194123877No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA18890.2220177167552269No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT18270.21473074034505008No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG18260.2146132084674666No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC18200.21390801720196562TruSeq Adapter, Index 12 (98% over 50bp)
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC17540.20615091328145477No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC17380.20427040324011878No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC17130.20133210630053133No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG17070.20062691503503036No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT16000.18805100413359613No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA15060.17700300764074736No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG14960.1758276888649124No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT14700.17277186004774145No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA14570.171243945639156No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG14380.16901083996506952No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA13960.16407450110656263No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT13800.16219399106522667No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT13730.16137126792214218No Hit
GCCACGCCACTCACCTGTGGCTGGCTTCCGGAAGCCTCTCAGGAGCGGGG13640.1603134810238907No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG13550.15925569412563922No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG13470.15831543910497123No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA13440.15796284347222075No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG13190.1550245465326333No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG13170.15478948277746632No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT13160.1546719508998828No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA13130.1543193552671323No Hit
CTCAATTGTCTCCTCTACTTTAGATCGGAAGAGCACACGTCTGAACTCCA12780.1502057395517099Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA12660.14879535702070795No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG12630.14844276138795745No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC12370.14538693257078653No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA12310.14468174130528555No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT12100.14221357187603206No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT12030.1413908487329476No Hit
GATTTCTCTAATAGCTGCCACATTAGGACCTGCTCCTAGATGGGTATAAA12000.1410382531001971No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA11980.1408031893450301No Hit
GTTGTTTTAAAAGGACAAAAAAGACAGTAATGAGTATCTAATGACTTACA11890.13974540244677863No Hit
ACACACACACACACACACACACACACACACACACACACACACACAGATCG11600.1363369779968572No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT11540.13563178673135623No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG11540.13563178673135623No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC11480.13492659546585523No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC11400.13398634044518726No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC11390.13386880856760375No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC10920.12834481032117936No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT10900.12810974656601237No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG10900.12810974656601237No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC10890.12799221468842886No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT10710.1258766408919259No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA10600.12458379023850744No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA10600.12458379023850744No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC10560.12411366272817345No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA10440.12270328019717149No Hit
GTCCCCACGCGGAAGGCAACTGCCTGAGAGGCGCGGCGTCGCACCGCCCA10440.12270328019717149No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC10420.12246821644200448No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT10410.12235068456442098No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT10260.12058770640066853No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA10230.12023511076791801No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT10220.12011757889033453No Hit
GATTGAAGAAGACGTCCACCTCGTCTGGAGGCAGCAGCTGCGCGGGTTCC10210.12000004701275103No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC10200.11988251513516752No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA10170.11952991950241704No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG10140.11917732386966655No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG10140.11917732386966655No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG10070.11835460072658208No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG10060.11823706884899855No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA10060.11823706884899855No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC10050.11811953697141507No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT10030.11788447321624806No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG10010.11764940946108109No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG9620.11306566623532467No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG9440.11095009243882173No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT9370.11012736929573723No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC9350.10989230554057025No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC9330.10965724178540324No Hit
GAACAGTGCATGGGCCTATCAGGTTCAGTTTTCTCCACTGGGCCAGCTCC9330.10965724178540324No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT9310.10942217803023625No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG9300.10930464615265276No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG9130.1073066042337333No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA9070.10660141296823232No Hit
GACAGATAACGTACCTCTTCCACTTCGACCGACAAACCTGAGGTCATTAA9040.10624881733548182No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG9030.10613128545789831No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC8770.10307545664072738No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA8720.10248779725280989No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC8710.1023702653752264No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT8560.10060728721147394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)