FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002434962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334939
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC32057613.729523555005077No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT23921010.244807251923927TruSeq Adapter, Index 25 (97% over 44bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT220760.9454636716419572TruSeq Adapter, Index 25 (97% over 41bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT128310.5495218504637595TruSeq Adapter, Index 25 (97% over 41bp)
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG68260.29234168430096036No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA66180.2834335286703421No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC66130.2832193903138369No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC59750.2558953360237677No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG58560.2507988431389428No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG57350.2456166949115159No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG56180.24060585736929316No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT52970.22685817488165644No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC52020.2227895461080568No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT51900.22227561405244423No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA51900.22227561405244423No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG51590.2209479562421117No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG51320.21979160911698337No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC50050.21435249486175012No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC49300.21114041951417148No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC48140.2061724096432498No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG47950.20535868388852985No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC46260.1981208074386526No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC45830.19627921757270744No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT44390.19011203290535642No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC43920.1880991323542071No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT42880.183645054538898No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT42850.18351657152499487No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG42740.18304546714068334No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG42660.18270284577027496No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT42180.1806471175478246No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT42100.1803044961774162No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA39360.1685697142409288No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA39320.16839840355572458No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC38870.16647115834717738No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA38700.16574308793505954No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA38020.16283080628658822No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG37720.16154597614755672No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC37340.1599185246381169No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA36980.15837672847127912No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC36690.1571347260035487No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC36270.15533596380890466No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA35750.1531089249012501No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT35250.15096754133619764No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA35190.15071057530839135No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG34650.1483978810581347No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA34550.1479696043451242No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC34470.14762698297471583No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA34190.14642780817828646No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT34030.1457425654374697No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG33980.14552842708096442No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG33780.14467187365494347No Hit
ATCCTCCGTGGTCATGCTCCAATAAATTCACTGCTTTGTGGCGCGACCCT33740.14450056296973926No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT33370.14291593913160044No Hit
ATCCAGGTTTCTGTTGTTACAGTCTCTCTTCTGCCTCCTAGGCCGTTGGC33260.14244483474728892No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA33100.14175959200647212No Hit
ATACAAAGAGCTAAATAGGCGAATTGATCTAAAACAGATACATTCAAATT32710.14008931282573123No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT32710.14008931282573123No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG32250.13811923994588296No Hit
CATTTCTTTCATCCAGCCATGTTATCCCTTATTTGATCCACTCGAAGAGC32220.1379907569319798No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA32110.13751965254766826No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC32000.13704854816335674No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA31930.1367487544642494No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT31470.13477868158440115No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT31260.1338793004870791No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA30970.13263729801934868No Hit
AAACTTTCTCAAATAAATTCATTTCCATTTTAATGTTATTTATTCCCAGG30830.132037710621134No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT30820.13199488294983294No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA30640.13122398486641407No Hit
CTCCCTGCCCCCCAGCAGAGGTTCTAGACGCTGGAGCTGACCTTGGCTAT30390.13015329308388784No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG29510.12638445800939554No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT29360.12574204293987978No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT29310.12552790458337457No Hit
CAACACCTACGAAGGAAACATCATGAAGAATAAAGAGAAACTGCAGAGCC29150.12484266184255777No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC28950.12398610841653679No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC28910.1238147977313326No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT28880.12368631471742944No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA28690.12287258896270953No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG28620.12257279526360218No Hit
CTTCACAGGTGCTTTCAAGAACAGGAGCAGAAGTCACAACAAGCTTCAGT28580.12240148457839797No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA28560.12231582923579588No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA28400.1216305864949791No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT28340.12137362046717282No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC28270.12107382676806545No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT28080.12026010101334553No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC27830.1191894092308193No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG27830.1191894092308193No Hit
AACCTCATTTCCCCATAAAGATTCAGAACACAAAGATCATCCTTTCAGAG27560.11803306210569099No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG27480.11769044073528258No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT27460.11760478539268049No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG27340.1170908533370679No Hit
CACCTGACTGTTTTCTCTTTTCCACGTTGAGTACACAGAACTAGACACAT26850.1149922974433165No Hit
AAAACAAAATGAAATGAATGGTGCTTACTTCAAGCAAAGCTCAGTGTTCA26600.11392160566079028No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT26490.11345050127647874No Hit
GTCATTGCCAATCATACGCATAAATTCCTAAAACAAACCCACTTTCAATA26280.11255112017915672No Hit
GTATCAAGTACCTTTGCTGAGCAAGTTGCGCAGCTTGTTGACCAGACATA26270.11250829250785566No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC26120.11186587743833992No Hit
GTCCTCTTCACCCTCACATTGCTCCCCAGGTGGCAGTTTTGGCACAGGAA26100.11178022209573782No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC26090.11173739442443678No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT26070.11165173908183469No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC25990.1113091177114263No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG25930.11105215168362001No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC25930.11105215168362001No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA25920.11100932401231894No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA25800.11049539195670637No Hit
CTTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATT25440.10895359578986859No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA25430.10891076811856755No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC25090.10745462729433188No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG24970.1069406952387193No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG24890.10659807386831091No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG24540.10509910537277418No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC24320.10415689660415112No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA24310.10411406893285007No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT24200.10364296454853854No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA24130.10334317084943119No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT24080.10312903249292593No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC24070.1030862048216249No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT23860.10218682372430286No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA23810.1019726853677976No Hit
CTTCTGACCAAGCAAACTCGTAGATGATGAATAGTCATCGTCATCCTGCA23660.10133027029828189No Hit
AAATCGTATTAAATTCTTACCAGCAGTATGTGAAGTGTCTGTCACAACAG23560.1009019935852714No Hit
AAAACTCCACCATTCAGAGTGCTTTTTCCTTCTGAAGGAAGCTGAGGAAC23470.10051654454356196No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC23440.10038806152965879No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC23430.10034523385835775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)