FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002434991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002434991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226927
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT17200014.018763952541594No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC63660.518857275127208No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA63100.5142930263984735No Hit
GGGGCCACACTTACTCTGCACGGCCTCGATCTTGTAGGGGATGTTGAGGC42950.3500615766056171No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA38370.3127325423598959No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA36600.2983062561994316No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT36230.2952905918608035No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG32340.2635853640844158No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG32270.26301483299332395No Hit
GGGACCCGAGCTGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA30510.2486700512744442No Hit
GTGTGCCTCCCACAGCATGACCTACCATCATTGGAAAGCAGTTTGTAGTC29830.24312774924669522No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA29250.23840049163479163No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA28640.23342872069813445No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT28180.22967951638524542No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT27440.22364818770798917No Hit
GGAGAGCCGAGCCAGGCAGACGTGCAGCCACTGGGCCCCAGCAGCCTGGC26360.2148457080168584No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC26190.21346013250992113No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC26120.21288960141882932No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC25530.20808083936534125No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT25350.20661375941681942No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG25180.20522818390988218Illumina Single End PCR Primer 1 (100% over 50bp)
GCTGCTCTTCCTCCTCTCCCCTACGTCTCTTGCTCCTAGATCTTCACGCT25100.2045761483772058No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC24730.20156048403857768No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA24540.20001189964847133No Hit
GGAAGACACCTTGTGCGTCGGCAGACACAGCCGCATGCAGCGGGGTGCGG23780.19381756208804599No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC23580.1921874732563551No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT23320.19006835777515696No Hit
GCTGCTCCATGGTCTCAATGATGCTCTTTTGCTGCACATTATCACAGCTT22990.187378711202867No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT22950.18705269343652883No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG22900.1866451712286061No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC22840.18615614457909885No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA22440.18289596691571708No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC22230.18118437364244164No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT22110.18020632034342712No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT21850.17808720486222898No Hit
GGAGGGGTGACCCAAACACTGATTCTGCTCAGACATCGTCGTGCACAAGC21650.1764571160305381No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG21250.17319693836715633No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT20680.1685511851968373No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC20530.16732861857306913No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA19890.16211233431165833No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA19860.16186782098690466No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC19740.16088976768789015No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG19260.15697755449183204No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG19030.1551029523353875No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT18940.1543694123611266No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC18380.14980516363239216No Hit
GGTAGCCTTAGAAAGCTGGGCTGACCTAAGGGCAGAGTTGGTGTTCTGCA18280.1489901192165467No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT18280.1489901192165467No Hit
GGACAGGAAGAGAATTAACCAACCGGCACTCACTTCCATTTTCATTGCCC18220.14850109256703944No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA18200.14833808368387033No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA18190.1482565792422858No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA17760.14475188825415042No Hit
CGAGACAAGGAGCAAACACGAGATCTTGTGCCCCCAACACAGCACTATCT17740.1445888793709813No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA17400.14181772835710682No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG17180.14002463064224685No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG17090.13929109066798595No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC17090.13929109066798595No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC17010.13863905513530958No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG17010.13863905513530958No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG16810.1370089663036187No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT16770.13668294853728055No Hit
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA16500.13448232861449785No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA16460.13415631084815968No Hit
GGAAAATGGCTGCTGCACCCCCAAGGAGGGCCGAGGAGCCCCTGCAGAAG16390.13358577975706784No Hit
AAGCAGTGAGCACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTC16070.13097763762636244No Hit
AGGCCACCAACACAAATCTGAATACTGAGAGGAGAAAGATACACACACAC15870.12934754879467156No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA15760.12845099993724157No Hit
GTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTG15740.1282879910540725No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC15620.12730993775505794No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT15500.12633188445604343No Hit
GGAACATTCTCCAACACTTCCACATGCAATTCTTCTCCAGTAAGCCAGGA15360.1251908222738598No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT15360.1251908222738598No Hit
GGAGTTTTAGAAGAACACCACCACTACCCCAACCAAAGTAACACAACACT15340.12502781339069075No Hit
GAAGAGGACGAAAAACGATTTCTTGTTACTGTTATAAAGGTATGCAAGGG15320.12486480450752163No Hit
GTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGCTGA15230.12413126453326075No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG15050.12266418458473895No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT14990.12217515793523169No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA14990.12217515793523169No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACTAGCCCCAAGTGGATGCACTTA14850.12103409575304805No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG14810.12070807798670988No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT14720.11997453801244899No Hit
AGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGATACTCACCTGCAG14700.11981152912927989No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA14640.11932250247977264No Hit
GCTGTGCCTTCAGGGTTCTCGGGCTGCCACGCCGATGGCGAGAGCGGCAG14500.11818144029758901No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG14440.11769241364808175No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT14340.11687736923223632No Hit
GGGTCCTGCCCTTGCACAGATGGGGTCTCATTCTGCCCCATCCCCATAGG14230.11598082037480631No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT14140.11524728040054544No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA14090.11483975819262271No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC14080.11475825375103815No Hit
AGGTGACATGTATTTTGCTCTGTTCAATTTGAGTTATGTCTGTTTTTAAG14070.11467674930945362No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT13860.11296515603617818No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA13720.11182409385399458No Hit
GGATTCTGTTGTTAGCTGATAAGAGTAACACAGCCAGAGCTGATTCAGAC13490.10994949169755007No Hit
GGACAGAGGAATTGAGAGGTCCTTCTGGGACTCTTTGAGCTTCTCAGCCA13470.10978648281438097No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC13390.10913444728170461No Hit
GGACAAGAACAGCAACGAGTACCGGGTGCGGCGCGAGCGCAACAACATCG13360.10888993395695098No Hit
AGGTACTCACAAGCCTTCCCTGAAGCCATCAATCAGTGCTTGGAAGAGAG13340.1087269250737819No Hit
GGTTATCATGTGAAACAAAACTCACCTGGGTCACTTTGATATGGTCTTGG13340.1087269250737819No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA13280.10823789842427463No Hit
GCACAAGTTAGGTTTGTTTTGTTTTGCCTCACAGTTTGCTGTTTGTCTCC13260.10807488954110556No Hit
GAAAGCAACAATTTTATTTACTTCTTGACGGTCCAAAGGGAAACTCTAGA12990.10587426961832286No Hit
GGGGAGGGTCCCCACGCCTGGCCTGGCCCCCCTCACCGGCCCCCGCCCTC12920.10530373852723106No Hit
GGGGTAACTTTTGGGTCATAATTCTTAGGCAATTTTCCTGCATAACGATT12810.10440718966980105No Hit
GGTTATAATGTGAAACAAAACTCACCTGGGTCACTTTGATATGGTCTTGG12700.10351064081237106No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA12610.10277710083811019No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC12570.102451083071772No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC12550.1022880741886029No Hit
GGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAAGCCAAGCCAA12460.101554534214342No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT12460.101554534214342No Hit
GGTGTTTAGGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCAC12430.10131002088958838No Hit
GGTATTGGTCAGAAGTTCAACACTGTAGCCATTGCAACATGCTCAGTTAA12350.10065798535691202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)