FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002435014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827331
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC32350117.703470252515828No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT1042515.7050966683102295TruSeq Adapter, Index 7 (97% over 36bp)
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG184671.0105996122213217No Hit
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA152130.8325256891061336No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA107270.5870310305029576No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC93450.5114016015708156No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGGTCCCGC88250.48294479763108056No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC86810.4750644519246923No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT80800.44217495352511393No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC79080.4327623183758169No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC78860.43155837667067437No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG78640.4303544349655317No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC76080.4163449314875083No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG75530.4133350772246517No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC74640.4084645857811201No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC74100.4055094561412245No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG71310.39024128633509747No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT68830.3766695798407623No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA63830.34930726836024784No Hit
GATCGGAAGAACACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT62700.34312338596565156TruSeq Adapter, Index 9 (97% over 35bp)
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG62630.34274031360492435No Hit
ACACACACACAAACACACACACACACACACACACACACACACACACACAC62110.3398946332109508No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC59460.3253926081262781No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC59390.32500953576555097No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT56220.30766183028690475No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT55160.30186102025303574TruSeq Adapter, Index 9 (97% over 35bp)
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC54000.29551296398955634No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC51300.28073731579007855No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA49370.27017546355859995No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT49090.26864317411569116No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG47680.26092700227818605No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT47410.2594494374582383No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG45900.2511860193911229No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT45560.24932538221044792No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC43960.24056944253668328No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT40330.22070440440182976No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG40260.22032133204110257No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA40000.21889849184411583No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA39970.2187343179752327No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG38650.21151066774437688No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC38290.2095405813177799No Hit
GCCACGCCACTCACCTGTGGCTGGCTTCCGGAAGCCTCTCAGGAGCGGGG38280.20948585669481884No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT37970.20778939338302696No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA37550.20549095921866373No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA37080.20291890193949538No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC36880.2018244094802748No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG36700.20083936626697627No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG35800.19591415020048367No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC35650.19509328085606822No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA35410.19377988990500353No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC34590.18929247082219916No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT34220.1872676597726411No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG33570.1837105592801742No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG33320.1823424437061485No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA33240.18190464672246026No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA32380.17719832914781175No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA31980.1750093442293706No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG31940.17479044573752647No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA31860.17435264875383824No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC31550.17265618544204636No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG31260.1710691713761765No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT31100.17019357740880003No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA30610.16751207088370962No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT30510.16696482465409934No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT30100.16472111511269716No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG29910.1636813472764376No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA29840.1632982749157104No Hit
GATTTCTCTAATAGCTGCCACATTAGGACCTGCTCCTAGATGGGTATAAA29690.16247740557129497No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA29570.16182071009576263No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC29190.1597411744232435No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA28860.15793526186552956No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT28780.15749746488184133No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG28610.15656714629150384No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG28300.15487068297971193No Hit
GATTGAAGAAGACGTCCACCTCGTCTGGAGGCAGCAGCTGCGCGGGTTCC28200.15432343675010166No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC28010.1532836689138421No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG27350.1496718437984142No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA27160.14863207596215464No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG26640.14578639556818113No Hit
GTCCCCACGCGGAAGGCAACTGCCTGAGAGGCGCGGCGTCGCACCGCCCA26540.14523914933857085No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC26350.1441993815023113No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT26310.14398048301046718No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT26230.14354268602677897No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG26210.1434332367808569No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT25960.14206512120683118TruSeq Adapter, Index 9 (97% over 35bp)
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC25590.14004031015727308No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT25480.13943833930470179No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG25350.1387269192062084No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC25100.13735880363218267No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT24890.13620958655000107No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT24520.134184775500443No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA24440.13374697851675477No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC24210.13248831218865112No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT23900.13079184887685918No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA23820.13035405189317095No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA23750.12997097953244377No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC23650.12942373330283347No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA23600.12915011018802833No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT23510.12865758858137907No Hit
ATTTGTCTCAATTAAGAGTGGGCTCTTTAACACCATTGTTTTAAAAAAAT23420.1281650669747298No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG23380.1279461684828857No Hit
GTCCTCTTCACCCTCACATTGCTCCCCAGGTGGCAGTTTTGGCACAGGAA23380.1279461684828857No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG23350.1277819946140026No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT23060.12619498054813277No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA23020.12597608205628866No Hit
GAACAGTGCATGGGCCTATCAGGTTCAGTTTTCTCCACTGGGCCAGCTCC22420.12269260467862693No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC22380.1224737061867828No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG22340.1222548076949387No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC22310.1220906338260556No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG22200.12148866297348428No Hit
GACAGATAACGTACCTCTTCCACTTCGACCGACAAACCTGAGGTCATTAA22160.12126976448164016No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT22140.1211603152357181No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA21980.12028472126834164No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA21610.11825991021878357No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC21510.11771266398917328No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG21490.11760321474325122No Hit
GAAATTAAGAAAGTCTGTGTTATCTTTTTATAGATATTGAAGCACAGATT21350.11683707002179682No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA21150.11574257756257623No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA21120.11557840369369315No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT20720.113389418775252No Hit
GTCCCTGGACAGCAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGG20350.11136460772569391No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC20210.11059846300423952No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT20210.11059846300423952No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC19790.1083000288398763No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT19750.1080811303480322No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA19520.10682246401992852No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG19480.1066035655280844No Hit
ATCCTCCGTGGTCATGCTCCAATAAATTCACTGCTTTGTGGCGCGACCCT19260.10539962382294177No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT19190.10501655146221457No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC19150.10479765297037044No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG19030.10414095749483811No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG18950.10370316051114987No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG18780.10277284192081239No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA18720.10244449418304619No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG18620.10189724795343592No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA18600.10178779870751385No Hit
AGTTCAGTGCAAAACTTAATTTTAATATTAAAATTTTTCTCATGCCCTAT18430.10085748011717637No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG18370.10052913237941019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)