FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002435020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185681
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACAGGGCGTTCAAGGATGACCCCTGGGCAGGTGGGGCCCGCACTGGC74053.988022468642457No Hit
AGACACCCCAGTTCCCACTTGGGCCCTGTTATCCTCACCCTGTATCACCT56633.0498543200435155No Hit
CCCCGGACTTTTCGAGGGCCTTTCCTACCTGGTCTTCTAGGAAGCGGCTG52362.819890026443201No Hit
CAGCAGGGCTGCTCACGGGGGTCCCCGGCACCCACCTTGACAAAGTACAG51312.7633414296562386No Hit
CTGCCATGGAGAGGTGTCTGGCCGCCGCTGTGATCGCTGCCTGGCAGGCT49572.6696323264092716No Hit
CTGTCTCGGGTGTATTCGGTTTGAACTGCATCTCTGGATTTCTTTCCGAA45202.43428245216258No Hit
CCCAGCAAATAAACAAAGAGCCAACCCACCTCCAGGTAACTGAGGATGCA45042.425665523128376No Hit
CAGGTGGGCTTTCTGGTCTTTAAGCTTTGATTGTAACATTTCTGCCTTCT43922.3653470198889495No Hit
CAAGGAGAGCCTAAGGGCACTGGTGCCACGTGTGGCAAAAAGGGCAGCCA40262.168234768231537No Hit
GGGTAATTTAGGAAGATAAGCTGCTTTATTTTTAACTGCAGTGTGTTTTG38912.0955294295054423No Hit
CCCAAGGACAAGCCCTCTTTCAACGCTCTATTTATTGCACAATGCAGGTT37712.0309024617489135No Hit
ACGTACCTGCCTGGAATTGATGAAGAAACCAGTGAAGAATCCCTCCTGAC36401.9603513552813696No Hit
TAAAATCTGCCTTTGTGAAATGTGCCAACTTTTACTCATTTTTCCTAGAC36381.959274239152094No Hit
TGATAAACAGAGCATACAACTCACGTAATTTTGTACTTTTTCTTCCTATT35531.9134968036578863No Hit
CGTACACAGCAGATAGTTTGAATGTTAAGTCTTTTTGCATTCTTTCAAAA35521.9129582455932486No Hit
CATGGTTTCTTGACATTCACCCCAGTCCCTCTGGAATAACCAGCTGTCCT35291.9005714101065805No Hit
CTGAGAACAGGAAGTTGGTAGCTCAGCTGGACTGATATGTGATTTATTCT32201.7341569681335194No Hit
GGGAACAGCTAAGGTGGCGCTGGTAACAGACTCACCACACGCTTTGTTGA31891.7174616681297494No Hit
GGCAGCTCATCTTCCCTACCTCTTCCACGTTGTTACTTTCTTAGTAGCCA29691.598978893909447No Hit
GACTTTCCCTGCTGCAGACCAAGTTGCAAAGGCATCAAGACCACGCTGTC29671.5979017777801714No Hit
ACACTGTACATGGTGGGTCCAGCTTGCTCACCAGTAGTTGTCTGAGGCCA29421.584437826164228No Hit
TCAGTGTAGATTTAGCTGACTTGGAATATAATTGACATTAAGACTACTGA29021.5628955035787182No Hit
GCATGTGGGACTGTGCTACTGAGCTGCTGAAAGACTGGGAATGTATGAAT28781.5499701100274126No Hit
AGGCTTTTGTTTTCTGTTGGGGTTTTTATAGAACCTGCTTTTTAATCCAA26421.422870406772906No Hit
CAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAAACCAGACCT26141.4077907809630494No Hit
TGACCATCACATGCTAATAGTGTATTTTTTTCCAGGTATTGAATTGAAAC25611.3792472035372494No Hit
GTGTGCCTCCAGCAGAGAAGCAATTCTGGCCTGGCAACCTATAGCCCACC25291.3620133454688417No Hit
CAGGGACTGCCCCTTACATAGGTTTGTGTCCCCCGTTCCTGTGACTTGCA25141.3539349744992757No Hit
CTAAGATGGAAGGCCCCCAGGGGGCTTGTGGCCTGAAGCTGGCAGGAGAC23811.2823067519024562No Hit
CCTTTTCATGCTTTTGTCTAGGGTATTTCAAAAGCAAGTGATATGTTAAG23621.2720741486743392No Hit
GTCAAATGAACTCTCTCAAGGTGAAATAAGTGAATAATTGTGTATTTATC23151.2467619196363655No Hit
GAGGGCAATTTTGGTGGCATTTTTTTCACCAATATATCAAATGTAATACA22861.2311437357618713No Hit
TGAAAGCTTAAACACTTTATGTCCAAACATTTTCTTTTTAGTGTATTCCC22771.2262967131801317No Hit
ACTGTGTGAACCTCATCAACCATCTCATGATTATCTTTAATAGAGTCCAG22681.2214496905983918No Hit
TTCTCTAGTCGGCGTGGCACAAGCCTAATGGAAGATGATGAAGAGCCAAT22531.2133713196288258No Hit
AAGTGTGGCTTGCATGAGCATAATTCTATTGCGCCTCTCTTCCTAGGCCT21811.1745951389749087No Hit
GGTTTGAATTCAGTACTCCCTTGGAACTAATGCCACATTTCTTTAACAGA19721.0620365034656212No Hit
TAAAATCTGCCTTTGTGAAATGTGCTCTAACACCAAGTTGCTAGACCAAG19111.029184461522719No Hit
CTTGGCAAAGGAAGTAGTGAGTGAAATTTCCTAAGTTATTGACTTTTTTA17860.9618647034430017No Hit
TTTCATATTGATTAGGCTGTTCCAATGAATATTTTTTAATTAAAAATTAA17190.9257813131122733No Hit
TCTTGAACCGCTGTCTTCCGGGCATGCCCGGGCACTGGCTGGGCACCTTC16020.8627700195496577No Hit
ACACTGTACATGGTGGGTCTGGTGGGTCTAGAATTGAGTAGACCAAGTCT14840.7992201679224046No Hit
AGCATTTGGATGCCTTATTGCGACAGATCCGGAATATTGTAGAGAAGCAC14620.7873718905003743No Hit
CGACGAGTTTATCAGGAAGTAACACCATCGTAAGTCAAGTAGCATCTGTA14590.7857562163064611No Hit
GAGGATGTGTGGCTTCCACTGATGTCTTACCGAAATTCTTTGCTAGCTGG13710.73836310661834No Hit
CTTCCGGAGAGCACTAAGCCACTTCCAGCCCTGCTGTGAACCAGGACCGT11340.6107248452991959No Hit
AATTTTTGACCAAGGATTTGCAGCGCTCAGTTTAATAAAACATTTAAATT10380.559023271093973No Hit
GTGAAAGAACTTGAAAGATTCATGGTCTCTAAATTTTTTTTTTTTTTTTT9430.5078602549533878No Hit
GACTTTCCCTGCTGCAGACCTCCAGGACAACGCTTGCAGATGTGGGTGAA7620.410381245253957No Hit
AAGTCAGTCCCATGGAATTTTCGCTTCCCACAGGTCTCTGCTAGGGGGCT6940.37375929685859083No Hit
GTGTGCCTCCAGCAGAGAAGGAATTCTGGCCTGGCAACCTATAGCCCACC6150.3312132097522094No Hit
GACTTTCCCTGCTGCAGACCCAGCCCATCCTGGTTATGAAAACCACTCAT5990.3225962807180056No Hit
CTAAGATGGAAGGCCCCCAGGTTGGATAACCCTGTCAGATAACCAGATGG5750.3096708871666999No Hit
ACGTACCTGCCTGGAATTGAACCCTCAACAGGTGGAGAGACCAAGTCTCT5550.2988997258739451No Hit
GACTTTCCCTGCTGCAGACCCTTCCGGCCTTCGTTCACTTCTGTGGGGCA5440.29297558716293No Hit
GTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTATAGCCCACC4740.2552765226382882No Hit
CAGCAGGGCTGCTCACGAACTCCCAACCTCAGCCTCCCAAAGTGCTGGGA4470.2407354548930693No Hit
CCTTCTCTCAACTGAGTCTAAACCTTAGAACTTTTACACTAGTCTTTTCC4050.21811601617828424No Hit
ACACTGTACATGGTGGGTCCAGCATGAGACACTTCCAAAGACCAAGTCTC3440.18526397423538218No Hit
CTAAGATGGAAGGCCCCCAGTCCTCTCTTTCAGAGCAGCCACCACTGGGA3380.18203262584755575No Hit
TTCTACCCCCACCCTCATCCAGCCCAAAAGCACAGAAACTAGGTCTGCTC3220.17341569681335192No Hit
CTAAGATGGAAGGCCCCCAGGACCAGATTGTACTTCTCCGCCTCGTCGGC3210.17287713874871422No Hit
CAGGTGGGCTTTCTGGTCTTTGTCCTTTCAGCCAGGGACAGAGACCAAGT3200.17233858068407645No Hit
CAGCAGGGCTGCTCACGTAGAGACTTCTGACTTGTGTTTCATCCACAGAG3090.16641444197306132No Hit
CTGAGAACAGGAAGTTGGTAGCTGCTCACCATCGCTATCTAGACCAAGTC3020.16264453552059716No Hit
GACTTTCCCTGCTGCTCACCATCGCTATCTAGACCAAGTCTCTGCTACCG2500.13463951615943473No Hit
CTAAGATGGAAGGCCCCCAGGCCGGGCCTATAACTCCTTTCCAGCAGGTA2210.11902133228494031No Hit
CAGCAGGGCTGCTCACGTCTAGTGGGTAGAGAGGCCTGAATGCTACTAAA1870.10071035808725717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)