FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002435021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185681
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCCTGTGACTCCTCAGCCTCTGTTTTCCCCCAGGGATGTCGCTGGGG70013.7704450105288103No Hit
CCCAAGGACAACAGTGTCAAGTCTGGACCATGCTTACCACTGAGAAAGCG59793.220038668469041No Hit
TTCCTGACTGTCTCCCATGCTGTCCCCGCCAGGCCGAGCGTCTCACCTCG55042.964223587766115No Hit
CCCTGGAGGCGGCGAGAACATGGTGCGCAGGTTCTTGGTGACCCTCCGGA51672.7827295199831967No Hit
CCCTCCCACACTTAAATATTGTTGAGTCTCTACTCAATGGATCTCTATTA46902.5258373231509954No Hit
CCAGTAACACCCATGTTGTTCTTCTGCAAAGGTGGATGCGAGCACGGCCC45862.469827284428671No Hit
GCCCCACAGAAGTGAACGAAGTGTTTCTTAAATAAGGAAATGCAGAGCAC45022.4245884069991006No Hit
GCCCTGTGCTGTCCTTAGACTGCCTTCCGTGTAAGCTATTTCTGTTGTGT44672.40573887473678No Hit
CCTGGGCCCATGGTAAAACTGCTCAACCAGATTTCGATCTCTCTTTTCAT42572.2926416811628547No Hit
ATGGAAAGCAGAGACGCCTGGTGGTACGGGGACCAGCCAACAGGCAGGAC39932.1504623520984913No Hit
TCTAGGAACCTCAAGGCAAAGTTTGCTTACCTTGTCATTGAATATACGGA39362.1197645424141403No Hit
CTCCACCTGTTGAGGGTTCAATATCCGAGTATTTGGAGTCACAAACAATG37752.0330566940074646No Hit
GGGAATTCCTAATGTTGGTGTCTTATATTGTTGCTCAAAGTCATATAAAG36591.9705839585094869No Hit
AAAGGAGGTGAGATTCAAAATTCGTGATTTATCTTACCGGTGCCATTCGT36101.9441946133422376No Hit
GTCAGTGCCTCAGTAGAGCCCATTAAACAGATACTTACCCACAGCCCCAT34661.866642252034403No Hit
TATGACATGCCAGAGGAGATTGAGAACTATGGTAAGAGCCTGGGGCCCAA34061.8343287681561387No Hit
TGATGGCAGTCACATCTTAACCAAAGAGAGCAAAGCCAAGAGCGTGAGCA33911.8262503971865727No Hit
GAGAGTGGAGGATTTAAGCCTGATTGAACAGTTTTCACAACCACATGTGT31531.698073577802791No Hit
AGCAACTTGGTGTTAGAGCACAAACATAAATAAGATAATCTATATTGATC29481.5876691745520544No Hit
CTACGGCTGCGGCTACGGCTGCTGGAGCGGGACCTGTGGCGATGGCGTTT28881.55535569067379No Hit
TTGTCACCATTGCAGAGGCAGAATTTTATCGGCAGGTTTCTGCAAGTCTC28801.551047226156688No Hit
ATGAAAACAAAACTATGCACGAAGTAAGATTTTTCTATCAAATTGTCTTA28551.5375832745407447No Hit
GAGAAATACCGATAAAAGTGAACCTATTAATCTCTATAGTCTTTTTGTTT27721.4928829551758123No Hit
AGATAGCGATGGTGAGCAGCTGGGGCTGGAGAGACGACAGGGCTGGTTGC27041.456261006780446No Hit
ATGTGCACAAAGGAGATACTAGATAAATATTTGAGCAAACTCAATACCTG26411.4223318487082686No Hit
ACTCAATTCTAGACCCACCAGATCCTTAACATTGGTCCGTATTTGAAGTC26031.4018666422520345No Hit
TTTTCACTCGCCCCAGATCCACCTGGCTTCCAAGCTGTGGGCGCTCTGTG26001.4002509680581212No Hit
CATGAAGCTATCCTCAGGCCGTAATCTTTTGGGCTCTCTCATAATAGTGG25661.3819399938604382No Hit
ACTTTGTGCAGACACAGTCAAAAATACATTTTTCCCTTACCCGCAATGCT24401.314081677716083No Hit
CATGTACTCTCCCACCTTATTTTCAATGATCTAGATCAATACTTTGTTTC23341.2569945228644825No Hit
GCATCAGGATCCCCTCAAAGATATACCATTGAGTCATGGACCAGCACAAA23331.256455964799845No Hit
CGAAATGCTCTGACTTGTGAGTCAGACAATCCTGTAAGAAGTGAAATGAC23241.2516089422181051No Hit
CTGCCTAGTCAAATTCCAATCATCTATTGTATCGAGTTATCATCTAATTT22951.2359907583436107No Hit
ATCTAAACCCCTGGCAACTATTTTAATATTTTTGCAAAACTTACTTCTCT22911.23383652608506No Hit
AGACATAAGGGCTAACTTACTTCAATTTATTTCAAGTAATCACTTATTCA22251.1982916938189692No Hit
AGCACTGTGTTGTTTTTACCCTCAAAACCTATTTCAGGTTAGTTTCAAAG21541.1600540712296896No Hit
GACCACCAAACTTGGGCTTATCTAAGCAATTAGTACAAACACCACAGTCC21401.1525142583247612No Hit
AGCAACTTGGTGTTAGAGCACATTTCACAAAGGCAGATTTTATGTAGAAC20061.0803474776633042No Hit
ACAGAAGGCAAAAGAAAAGTGATTACCTTGCTTGAAACCAATTTCACATT16190.8719255066484993No Hit
GGGACACAGCTCTCCTGTTGCCCCCCTCGCCTCGGCCGTCACCCCGAGTC16170.8708483905192238No Hit
TAAAAATTGCCCTTAAGAATCCCAAAATGAAATTAAATAGGACATTTCCA15880.8552302066447294No Hit
ACTCAATTCTAGACCCACCAGACCCACCATGTACAGTGTTGTAGAACCAT14780.7959888195345781No Hit
CTGTTTTACCTGCAATTGGGGCATTTGCCATCAGGGGGCAGTGATGCCTC14570.7846791001771856No Hit
AGTAGAGGGGCTCAACTGACAAAATACTTGTAATAGTTATAATATATACC14320.7712151485612422No Hit
AGGTGAACCTTAATGACAATTCAGTTGGTAAACATTAGGGATAAATATAA14300.7701380324319667No Hit
AAGACTGGTACTGGTTAGTAAAGGTTGATAATATTCCAATAAATAACTTC11970.6446540033713735No Hit
TGGCAAGTATCCCAAGGCTCCGGAAGGGGGCGAAGGTGCCCAGCCAGTGC8850.4766238872043989No Hit
TTGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGA7300.3931473871855494No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6410.34521571943279067No Hit
AGATAGCGATGGTGAGCAGCCTCAGAAGTGGGGGCTGGTGTTTATCCCCT6140.3306746516875717No Hit
GCCCCACAGAAGTGAACGAAGGCCGGAAGGGTCTGCAGCAGGGAAAGTCT5640.3037467484556848No Hit
CTCCACCTGTTGAGGGTTCAATTCCAGGCAGGTACGTTGTAGAACCATGT5570.2999768420032206No Hit
TTTTCACTCGCCCCAGATCCAGAGGTCCAGGTACCCCCGATACTTCCACC5470.2945912613568432No Hit
GGAGTAAAGATGGGGGCTGGGCTCGGTTGCTCACACCTGTAATCCCAGCA4340.2337342000527787No Hit
CCCACATTGTTAGGGCCGAGGCTAGACCTTCTCTGTCCATTTAGGTGTGA3920.21111476133799364No Hit
TTTTCACTCGCCCCAGATCCCAGCTGCAACCTTTTTAGGGTCTTCTCCTT3580.19280378714031055No Hit
CTGTCCCTGGCTGAAAGGACAAGCAGGAGGCCAGGCAGGAGCTGGTGGCC3400.18310974197683125No Hit
TTGGAAGTGTCTCATGCTGGACCCACCATGTACAGTGTTGTAGAACCATG3370.18149406778291802No Hit
CTGTCCCTGGCTGAAAGGACAAAGACCAGAAAGCCCACCTGTGTAGAACC3210.17287713874871422No Hit
AGATAGCGATGGTGAGCAGCTACCAACTTCCTGTTCTCAGTGTAGAACCA3030.1631830935852349No Hit
GGAGTAAAGATGGGGGCTGGGGAAAAGGAAGAGGAGACAAATTCTGTTGC2870.15456616455103106No Hit
AGATAGCGATGGTGAGCAGCAGGGAAAGTCTGTAGAACCATGTCGTCAGT2600.1400250968058121No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA2550.13733230648262343No Hit
ACTCAATTCTAGACCCACCAGATCCTTATCATTGGTCCGTATTTGAAGTC2510.13517807422407246No Hit
TTTTCACTCGCCCCAGATCCCCAGCTCCCATGTCTAAAGGTAGAGAGCCC2360.1270997032545064No Hit
TTTTCACTCGCCCCAGATCCTCACACCAGCACCAACTCCCTGTAAACCCA2330.12548402906059317No Hit
ACTCAATTCTAGACCCACCAGGTCCTTATCATTGGTCCGTATTTGAAGTC2230.12009844841421578No Hit
ACTCAATTCTAGACCCACCAGATCCTTAACATTGGTCCGTGTTTGAAGTC2130.1147128677678384No Hit
TGGCAAGTATCCCAAGGCTCTGGAAGGGGGCGAAGGTGCCCAGCCAGTGC2080.1120200774446497No Hit
GGAGTAAAGATGGGGGCTGGGGTCTTCGTTGGGGAGTGTCCTGTGCACCG2070.11148151938001195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)