FastQCFastQC Report
Wed 8 May 2024
EGAF00002435040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443153
Total Bases145.7 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC28409019.685369465330428No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC897646.219991920468585TruSeq Adapter, Index 10 (100% over 50bp)
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA129060.8942918734188267No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG114030.7901449118700512No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC105150.7286129745079004No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC100730.6979855912713343No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA93460.6476097821921861No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC80040.5546189489264132No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT78160.5415919171425344No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA75320.521912784022207No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT73520.5094400940163656No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC73180.5070841414597066No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT73170.507014848737452No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG70530.48872157006221795No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA70060.48546481211624826No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC66790.4628060919389697No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT66160.4584406504369253No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG65000.4504026946553831No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA62530.43328739225847845No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG61610.42691246181104847No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT58620.40619393785690083No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA57100.39566144407419035No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG55570.38505965756922517No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGGTCCCGC55360.383604510401877No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC55340.38346592495736764No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC55200.3824958268458022TruSeq Adapter, Index 10 (98% over 50bp)
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC54350.3766059454541549No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA53160.36836011150584863No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT52680.3650340608376243No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC52320.36253952283645596No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG51730.3584512522234302No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG49010.3396036317701588No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC48590.3366933374354625No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG48150.33364445765625683No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA47680.33038769971028714No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT47290.3276852835423548No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC46770.32408206198511175No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT46600.3229040857067823No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA44520.30849119947781No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC43850.30384858708674684No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACATATACCATCTGTTGAGCCCC43010.2980279984173542No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC42160.2921381170257069No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT42130.29193023885894287No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC41780.2895049935800293No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA41430.28707974830111566No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC41030.28430803941092875No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG40500.280635525131431No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC40230.27876462163055477No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA40150.2782102798525174No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA39760.2755078636845851No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG39560.2741220092394916No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG39060.27065737312675786No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA38810.268925055070391No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA38470.2665691025137321No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT37290.2583925612876805No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG35710.24744431117144197No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG35700.2473750184491873No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG35440.24557340767056576No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT35430.2455041149483111No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT34070.23608030472167538No Hit
CAACACCTACGAAGGAAACATCATGAAGAATAAAGAGAAACTGCAGAGCC34010.23566454838814735No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT32850.22762659260660512No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT32730.22679507993954903No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT32290.22374620016034336No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC31970.2215288330481938No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA31680.21951934410280824No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC31680.21951934410280824No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG31630.21917288049153483No Hit
AAACTTTCTCAAATAAATTCATTTCCATTTTAATGTTATTTATTCCCAGG31470.21806419693546006No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC31290.21681692793487592No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA31100.2155003662120371No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA30810.21349087726665156No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT29460.20413635976227051No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT29390.2036513107064878No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA29090.20157252903884756No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT29020.20108747998306484No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC28330.19630628214749235TruSeq Adapter, Index 10 (98% over 50bp)
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG28260.19582123309170962No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG27950.1936731587018147No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA27770.19242588970123056No Hit
GTCCCTGGACAGCAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGG27430.19006993714457165No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG27110.18785257003242206No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT26910.18646671558732858No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC26640.18459581208645237No Hit
CCACAAGAGGTCCCAAACCCCCCAGTACCTGTGTGGTTGCCCTCAGAGGC26580.18418005575292432No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT25970.1799531996953892No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC25800.17877522341705973No Hit
GCAACAGACTTTAAAGAAGTTGTGTTTTACAATGCAGAGAGTGGAGGATG25740.17835946708353168No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG25650.1777358325832396No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA25510.17676573447167415No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA25310.1753798800265807No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA25040.1735089765257045No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC24870.17233100024737502No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT24750.17149948758031894No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT24190.16761909513405715No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG24160.16741121696729314No Hit
AACGGTGTTACGGCATAAATGTAGTCTTTTCCCATTTCACCAATATATTC23950.16595606979994498No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA23510.1629071900207393No Hit
GAAAGGAACAGGTATTTAGCATTGCAGCTAGTTTACTGGCATTATCAGCA23280.16131345740888178No Hit
CATTTCTTTCATCCAGCCATGTTATCCCTTATTTGATCCACTCGAAGAGC23210.16082840835309908No Hit
AAATCGTATTAAATTCTTACCAGCAGTATGTGAAGTGTCTGTCACAACAG23040.15965043207476962No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA22860.15840316307418548No Hit
GTCATTGCCAATCATACGCATAAATTCCTAAAACAAACCCACTTTCAATA22400.15521569785047046No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC22220.15396842884988632No Hit
ACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCC22150.15348337979410362No Hit
GCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGAC21940.15202823262675544No Hit
GCAAGTAGTACAGATAGGTTAGCACCATGCTTTGTATTTTATTTAAAAGA21610.14974157279235117No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC21540.14925652373656847No Hit
ATGATGATAGGTTTTACCCATCCACTCACAAGCCGGGGGATATTTTTGCA21510.14904864556980446No Hit
ACCCCATCTGCTTCTTTCACGCAGGCAAGTCCGCCCTGCACTGGGCCGCC21060.1459304730683441No Hit
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC21020.14565330217932543No Hit
ACTTGTTTATATTACTTTCTCCAGTTTTATAAACAGTTCAATAATTTTCT21000.14551471673481606No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC20710.1435052277894305No Hit
GAAAACCAAAGCCTTTTTCATGTTAGATGGGATCCTTTCCAAATACTTTG20690.14336664234492116No Hit
GCAAAGGAAAAGGTCTAGGAGAATATGTAAATTTAACATTGATTAACTGA20650.14308947145590245No Hit
GCCAAGTCCTACAGACTCCAACTTCTACCGTGCCCTGATGGATGAAGAAG20550.1423965442333557No Hit
AGAAAATGTGTGGGAGATTGTTAAGTGTGATAAGTAAAACCCATGTACAT20540.14232725151110104No Hit
AAACCTCTCATAATGAAGGCCCCCGCTGTGCTTGCACCTGGCATCCTCGT20320.14080281162149821No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA19950.13823898089807526No Hit
AAAAGGCAGCTTGGACACGGCTTTACCTCCAATGGTGCAGGCTCCAAGTA19870.13768463912003787No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC19790.13713029734200047No Hit
CTTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATT19780.1370610046197458No Hit
AAATTAATACTTTTAAAAGTCATTACTGCTAGGTTTTTAATGCTTTAATG19510.1351901011188696No Hit
ATTACTTTTCCAAGGACGGTTGAAGAAATTGCTTGCTTCCACTAAAAATG19210.13311131945122934No Hit
ATTCTGAGAAAATTCCAAAATTGTGTCGACTCTTGTCCAAGCATCAGGAT19170.13283414856221065No Hit
GCTTATAAAAATGTGTGGGTAATCTGCTTACCTGTCAGCAATACGACCAA19070.1321412213396639No Hit
AAATAAGACTGTCCTCATGGCTCTGTGACTGTGCCTCTTGTCAGGTGTAT18970.13144829411711717No Hit
ACAGTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGT18530.12839941433791152No Hit
AATAATAATACTGCCTACCTCTTAATGAAAAGGCACCCTAGAAGAAGAAC18300.126805681726054No Hit
GTTCTAATTGCACTGTTTTTTCTCATTCCTTCCCCAGGCTGGATGATAGA17880.12389538739135768No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC17730.12285599655753757No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT17700.12264811839077354No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT17560.12167802027920809No Hit
CCACATGAGATTCCAGAAGAAATGTGTGATTAAAATCCAAATTCATGCTG17230.11939136044480385No Hit
CTCTTCCTGTCCAGGGATCACTGGAACTTGGGGCAGATACTGGTACTTTG16880.11696611516589024No Hit
GACATCAGTTTGCCAGTTGTGCTTTTTGCTAAAATGCATGTTTCCAATTT16750.11606530977657947No Hit
CACCGGGCCCTGGCCAGCCTCACCTTGCCAGCCCGTGGGGCAGACACAGG16670.11551096799854209No Hit
AAAACAAAATGAAATGAATGGTGCTTACTTCAAGCAAAGCTCAGTGTTCA16480.11419440627570326No Hit
AATATTAATCTTCAACCATTTCTTTCCATAATCAATTCCATAAACAGATA16410.11370935721992056No Hit
GTTTTACTAGTGGTCCTTTGGCGTGCTCCTCTGGGAGCTGATGACAAGAG16400.11364006449766588No Hit
CAGCCCTCACGTCTCCCCTGGCATCCCAGCCTCGCGCTCACCCTGTTGTT16240.11253138094159108No Hit
AAAACTCCACCATTCAGAGTGCTTTTTCCTTCTGAAGGAAGCTGAGGAAC16070.11135340466326162No Hit
AATGAATGATTCAAAAGAATACATTAGCTTAAGTTTTGTTTTTTTTAATA15840.10975967205140412No Hit
GCTCGTCCCGCCCGCAGCCCTAGGCGGCGTCGCCGCAGCCGATCCCGGAG15800.10948250116238542No Hit
AATAATATTGACTTTTCTTTCTTTCCAGACACTACAACAACAGGAGTGCA15650.10844311032856531No Hit
CTATGTTGACTTCTCTAAGGAGCTTCATGACAGACTCACCGGTGTTTCCT15380.10657220682768909No Hit
CACCAGGATGCGGATAAATAATGTTTTCAAGGATTTCCTAAAGGAATTTA15280.10587927960514236No Hit
AACATACTTGCTCCTCCTTAATGTATACATTTTTCTTTTCACTAATTTAG15260.10574069416063302No Hit
CCACAGCTGTGCCCAGTGGGGCACTGGGGCCGCGCGTGCCCCCCTCACTG15260.10574069416063302No Hit
ACACTGGAAATTCCAACATGCCTGGGGGGCTCCCAAGGCAAGCTTACACC15120.10477059604906756No Hit
AATAATTTCACAGAAGTCAAATGCAATTTTAGAAGATACTCTACAAAGTT14630.10137525265858853No Hit
CGTATTTCTTATAGGGAATAGGTAAATTCGTTCCTTGGGTTTCTTTCAAC14620.10130595993633384No Hit
CCACAGCTGTGCCCAGTGGGGCATTGGGGCCGCGCGTGCCCCCCTCACTG14550.10082091088055112No Hit
CCCCAAACGAAAGTAATCTGTTAAATTTTTTATGTACCCTAGGTGGAACG14450.10012798365800438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)