FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002435041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443153
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT27328118.936384430479652No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA134750.9337194323817364No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT115630.8012317474307991No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC110470.7654767027473871No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG96910.6715157713700488Illumina Single End PCR Primer 1 (100% over 50bp)
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT96630.6695755751469179No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT92440.6405419245222094No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC86620.600213560169989No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA85850.594878020556379No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA84710.5869786502193461No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC83940.5816431106057363No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT77800.5390973791413661No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT74700.5176166352424171No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC72800.504451018014029No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC71410.4948193296206293No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC70830.4908003517298582No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT67360.4667557771074862No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA65930.45684691782506776No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC65780.4558075269912476No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC65510.4539366234903714No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA65140.4513727927669485No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA60020.41589491897255526No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG57830.40071981279878155No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA57520.39857173840888666No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA55930.38755419557039344No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC55370.3836738031241317No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGGTCCCGC53960.3739035292862226No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG53210.368706575117122No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT53140.36822152606133934No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG53050.3675978915610472No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG52390.3630245718922387No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT52110.36108437566910784No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG52110.36108437566910784No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC51520.3569961050560821No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA48470.3358618247684064No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT47530.329348308876467No Hit
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA44240.30655100325467916No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT43970.30468009975380295No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA43740.3030863671419454No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA43460.30114617091881457No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT43340.3003146582517585No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG43250.2996910237514664No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA43000.2979587056950996No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA42770.296364973083242No Hit
GGAAGACACCTTGTGCGTCGGCAGACACAGCCGCATGCAGCGGGGTGCGG42610.29525628952716726No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC41950.29068296985835873No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA41650.28860418819071854No Hit
GGGACACCGATGGGCCTGCATCTGACCTGTTGTGCTCACTGCTTAGGACC40560.281051281464959No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT40530.280843403298195No Hit
GGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGGAGCTGCACTG40450.2802890615201576No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT39790.27571574185134906No Hit
GCTGCTCTTCCTCCTCTCCCCTACGTCTCTTGCTCCTAGATCTTCACGCT39370.27280544751665275No Hit
GAAGAGGACGAAAAACGATTTCTTGTTACTGTTATAAAGGTATGCAAGGG38560.26719273701402413No Hit
AGGTACTCACAAGCCTTCCCTGAAGCCATCAATCAGTGCTTGGAAGAGAG38250.2650446626241293No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT38070.2637973936235451No Hit
GGTATTGGTCAGAAGTTCAACACTGTAGCCATTGCAACATGCTCAGTTAA35310.24467260228125504No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC33500.23213061955315895No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT33050.22901244705169863No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT32500.22520134732769154No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT32490.22513205460543687No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG32340.22409266377161674No Hit
AGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGATACTCACCTGCAG32170.22291468749328727No Hit
GGGTCCTGCCCTTGCACAGATGGGGTCTCATTCTGCCCCATCCCCATAGG32160.2228453947710326No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT31910.22111307671466573No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC31440.21785631876869602No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG31390.21750985515742266No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG31180.2160547079900745No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC30950.21446097537821698No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT30630.21224360826606745No Hit
GCGGAAGCACCAGCTGAGAAGGTGGAGGGGACAGGATGGTACCTACCGTA30480.21120421743224732No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG30450.2109963392654833No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA30310.21002624115391785No Hit
GGGGTAACTTTTGGGTCATAATTCTTAGGCAATTTTCCTGCATAACGATT30170.2090561430423524No Hit
GGACAGAGGAATTGAGAGGTCCTTCTGGGACTCTTTGAGCTTCTCAGCCA29680.20566079965187337No Hit
ACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCC28750.19921657648218866No Hit
AGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACACCCAGCT28720.19900869831542461No Hit
GAGAAATCAATGTAAACACCATCTTACCAATGGGATTGGTGGACGTCTCC28420.1969299166477844No Hit
GGATTCTGTTGTTAGCTGATAAGAGTAACACAGCCAGAGCTGATTCAGAC28380.1966527457587657No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC27920.1934652805350507No Hit
GCAGGAGATGATCCGGCAACTGGAACAGACAAACTATCGACTGAAGTTGT27480.19041640075584504No Hit
GCTGAACTTAAACTTACCAGATTACATTATAATGCATTTTTTAATTTTCA27440.1901392298668263No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT27430.19006993714457165No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA27310.18923842447751554No Hit
GGCCACACTCGTTGACATCCTGCCGGCAGGTGGGGCCATGGAAGCTGGGT26960.18681317919860196No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT26890.18632813014281924No Hit
GGATTCACATTAACTCTATGACCATATTTTATTCCAGACACTTCTGAGAA26720.18515015386448977No Hit
GGACAAGAACAGCAACGAGTACCGGGTGCGGCGCGAGCGCAACAACATCG26700.18501156841998043No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT26660.1847343975309617No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG26430.18314066491910422No Hit
GAGATCACTCACCCATCGCATACCTCAGGGCAGATCAATTCCGCACAGAC25840.17905239430607842No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC25410.17607280724912744No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC25200.17461766008177929No Hit
GTCATTGCCAATCATACGCATAAATTCCTAAAACAAACCCACTTTCAATA23900.16560960618867163No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA23740.16450092263259683No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA23720.16436233718808746No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA23510.1629071900207393No Hit
GGACACAGACACAACAGGCACAGCTTTGAAGGCTAGAATGAAAAGAAATG23370.16193709190917388No Hit
GAAAACCAAAGCCTTTTTCATGTTAGATGGGATCCTTTCCAAATACTTTG23370.16193709190917388No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG22840.15826457762967613No Hit
AGTGTTCATTTAATTTTGGTTTATTTTCTCCTTTACAGATCTATATGATC22520.15604721051752654No Hit
GCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGAC22500.1559086250730172No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG22500.1559086250730172No Hit
GAGAATCACAATCACTGCAGCCTCACAGGTGTGGCCAGCTCGCTCCCGCA22410.15528499057272516No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT22250.15417630701665036No Hit
GTAGTATTTCTCCATTACCTAGGCTGAGAATTCTCCTCATACATTCTTTC22240.15410701429439566No Hit
GAGAAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGGAAATCACCAAA22040.1527211598493022No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC21890.15168176901548208No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG21710.15043450001489794No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA21610.14974157279235117No Hit
GGTATTCTATGTCTTTTAGGCTTACAGATACTTTTTACACTCTTACAAAA21550.14932581645882315No Hit
AACGGTGTTACGGCATAAATGTAGTCTTTTCCCATTTCACCAATATATTC21170.14669269301314553No Hit
ACAGTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGT21000.14551471673481606No Hit
GGTGAGAATTTGGGGCCTGGAAAAATGAAGTCATTGGAAGATAAGATTCA20940.145098960401288No Hit
GGAGCAGAATCCCGACCTCAAAGACGCCCCTTGCCTTGTACACCAGGCGA20590.14267371512237442No Hit
AGAGGCAGATACTGTTGCAGCACTGCCAGGTCTTGGGGAGGTGGGTGGGC20450.14170361701080897No Hit
GAGGATCAAGAGCGTCATTTACTTGTGAAAGTTATTGATAGGATACTGTA20290.14059493345473417No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC20090.1392090790096407No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC20010.1386547372316033No Hit
GACATCAGTTTGCCAGTTGTGCTTTTTGCTAAAATGCATGTTTCCAATTT19650.13616019923043504No Hit
GTAGAAGACTCTTATGAGCGAGATAATGCAGAGAAGGCCTTTCATATAAA19470.13491293022985087No Hit
GAACACTCGGCCGGCAGAAGGCTGCCACATGCAACTCAGGAACTCACTGC19240.13331919761799338No Hit
CAGCCCTCACGTCTCCCCTGGCATCCCAGCCTCGCGCTCACCCTGTTGTT19210.13311131945122934No Hit
GAAGGCAGTTATGCCATTTGTAGAATGGTAATAACCAGTTGGTATTTGGG19110.13241839222868262No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA18950.13130970867260783No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGTGGGGGG18930.13117112322809849No Hit
GGTGCCTACCATGGTCAATGGGGTAGACCTTCAGCCCATCCAGCTCAAAG18650.1292309270049676No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG18610.1289537561159489No Hit
AGAGTGGAAGGCCGAGAGATCATTTCTAATTTGGCAAAGGTATTTACATT18560.1286072925046755No Hit
GGGAGATCCCTCTGGGTGAGGCTCCCGGGGTCACGTGTGTCTCCTTCCCG18280.12666709628154466No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG18120.12555841272546986Illumina Single End PCR Primer 1 (98% over 50bp)
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC18020.1248654855029231No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGCGGGGGG17780.12320246016881092No Hit
GAGGGACCAGGGTTGGAAATAGAATGCGGGAAGGAGCAGCTTGGCTAAAC17680.12250953294626418No Hit
GATGTTATCCGTGCGCAGGATCTGCTTCTTGAGGTGGCTCATGAGGTCTC17600.1219551911682268No Hit
ACCCCATCTGCTTCTTTCACGCAGGCAAGTCCGCCCTGCACTGGGCCGCC17170.11897560411127579No Hit
GAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCCAACCCCACTCCCA17110.11855984777774775No Hit
GGGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGG17070.11828267688872905No Hit
ATTCTGAGAAAATTCCAAAATTGTGTCGACTCTTGTCCAAGCATCAGGAT17050.11814409144421971No Hit
AATGAATGATTCAAAAGAATACATTAGCTTAAGTTTTGTTTTTTTTAATA16790.11634248066559817No Hit
GGAAGAGCTTTCGACAGGCTCTACATGAAGTGCATCCCATCAGTTCTGGG16690.11564955344305144No Hit
AAATAAGACTGTCCTCATGGCTCTGTGACTGTGCCTCTTGTCAGGTGTAT16630.11523379710952338No Hit
GGAAAATGCTTTACCTTAGAAGCAGTTCCAAGAGCAGAAGCACTTGAGGG16420.11377864994217522No Hit
AAAAGGCAGCTTGGACACGGCTTTACCTCCAATGGTGCAGGCTCCAAGTA16360.11336289360864718No Hit
AAACCTCTCATAATGAAGGCCCCCGCTGTGCTTGCACCTGGCATCCTCGT16360.11336289360864718No Hit
GAGAGAGAGAGAGAGACAGAGATTTCATACCAGAGAGCTCAGGAGGGGAG16220.11239279549708173No Hit
AGGTGACATTTTAATTTACAAATTGGCATCATTTTGGTCGACAAATACCC16140.11183845371904434No Hit
GCTTATAAAAATGTGTGGGTAATCTGCTTACCTGTCAGCAATACGACCAA15950.11052189199620553No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAT15700.10878957393983867No Hit
AGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAGACCCCAAG15540.1076808903837639No Hit
CGAGAAATCAAGTCAGCTGCCTGTTGCCTAACTTTAGCAGATTTGTTATT15500.1074037194947452No Hit
GACTATTACTGAGCGGAATATTGATACTTACAGGGCAGGATGTTTTTATA15450.10705725588347181No Hit
GAATCTTGATGTGGGTGACAGCTCCATTTCTTCTAAAATAGTCCATTGGA15350.10636432866092506No Hit
AATGTTTTGTCAAAGTTTCCAAGGTAGCCAAGTATTTCACCTTCAGGTCA15200.10532493782710495No Hit
GTGCCTGCCTGCCAGGTAGTGCTGCCCGCTGGCCGGGGTGCACGAGCCCC15080.10449342516004886No Hit
GAGCTGTGCTACAACACTGCATGTGCACTGATAGGCCAAGGCCAGCTGAA15020.10407766882652082No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC14830.102761107103682No Hit
AGTTAACAAGCTGTTCGAATTCATGCATGGTAAATCTCTTTCTTTACTAT14830.102761107103682No Hit
AAAGATCTGCTGCCTAGACTCACCCCCATCTTAAAGAACAGACATGAAAA14710.10192959443662591No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGAT14660.10158313082535254No Hit
GAGGAGCTGTAAGGAATAACAATCAAAAGGATACGCTTTGGCTTTCTCTG14660.10158313082535254No Hit
AAAACTCCACCATTCAGAGTGCTTTTTCCTTCTGAAGGAAGCTGAGGAAC14520.1006130327137871No Hit
GCACCTGGTGTGAGGGTTTTTGGAACTGGAGATGTTGGTCCACTGTACCT14490.10040515454702308No Hit
GAGGATGAAGATGACTTCCTTTCTCGCTGAGGTCCTGGAGATTTCTGTAA14440.1000586909357497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)