FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002435052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340420
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC28466021.236627325763564No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC853996.37106280121156TruSeq Adapter, Index 11 (100% over 50bp)
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA118730.8857671476104504No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC115310.8602527565986781No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC114640.8552543232718103No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG103200.7699079393026067No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC92170.6876202981155161No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT90330.6738932573372525No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA81230.6060040882708405No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC76440.570269020157861No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA74310.5543784783873711No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT69210.5163307023171841No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC68350.5099148028229958No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC62610.4670924038734128No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA62020.46269079840646965No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG60420.45075424120797963No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG59120.4410557884842064No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT58390.43560973426239535No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG58230.4344160785425464No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC54380.40569373778367973No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT53600.3998746661494158No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT52640.39271273183032185No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC51600.38495396965130335TruSeq Adapter, Index 11 (98% over 50bp)
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC51390.3833872965190015No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC49620.37018248011817195No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC49610.37010787663568134No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA49570.3698094627057191No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA48730.3635427701765118No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA46370.34593634830873904No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC46080.34377284731651275No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT45610.34026648363945633No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA44970.33549186076006027No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG43640.32556959758881543No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG42920.32019814684949494No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC41460.3093060384058728No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT40270.3004282239894958No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC40000.29841392996225063No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG39870.2974440846898733No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG39370.2937139105653452No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC38910.2902821503707793No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT38850.28983452947583593No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA38240.2852837170439116No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG36790.27446621208278No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG35580.2654391907014219No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG34950.2607391713045165No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT34940.26066456782202596No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA34800.2596201190671581No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC34690.2587994807597619No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG34300.2558899449426299No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG33910.25298040912549796No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT33750.25178675340564893No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG33700.25141373599319616No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG33320.2485788036585548No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC33220.2478327688336492No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT33050.24656450963130958No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA31830.23746288476746094No Hit
CCACAGCTGTGCCCAGTGGGGCATTGGGGCCGCGCGTGCCCCCCTCACTG31610.2358216081526686No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA31600.235747004670178No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA30120.22470568926157472No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT30000.223810447471688No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG29660.22127392906700882No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC29500.22008027334715985No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA29210.2179167723549335No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA28830.21508184002029218No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC28060.2093373718685188No Hit
CAACACCTACGAAGGAAACATCATGAAGAATAAAGAGAAACTGCAGAGCC28030.20911356142104712No Hit
AAACTTTCTCAAATAAATTCATTTCCATTTTAATGTTATTTATTCCCAGG27890.20806911266617925No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA27690.206577043016368No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG27590.20583100819146238No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA27550.20553259426150014No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC26770.19971352262723624TruSeq Adapter, Index 11 (98% over 50bp)
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC26770.19971352262723624No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT26520.1978484355649722No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA26030.19419286492293458No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT25850.19285000223810447No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC25530.19046269079840647No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT25210.18807537935870847No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT25090.1871801375688217No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG25050.18688172363885946No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA25050.18688172363885946No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA24700.18427060175168977No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG24700.18427060175168977No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT24690.1841959982691992No Hit
CCACAAGAGGTCCCAAACCCCCCAGTACCTGTGTGGTTGCCCTCAGAGGC24610.1835991704092747No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT24390.18195789379448232No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT24380.18188329031199177No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA24330.18151027289953894No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA24220.18068963459214277No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT24190.18046582414467108No Hit
GTCCCTGGACAGCAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGG23810.1776308918100297No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC23520.17546739081780338No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT23370.17434833858044493No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC22730.16957371570104893No Hit
GAAAGGAACAGGTATTTAGCATTGCAGCTAGTTTACTGGCATTATCAGCA22700.16934990525357724No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC22510.16793243908625655No Hit
AACGGTGTTACGGCATAAATGTAGTCTTTTCCCATTTCACCAATATATTC22300.16636576595395475No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA22170.1653959206815774No Hit
AATAATAATACTGCCTACCTCTTAATGAAAAGGCACCCTAGAAGAAGAAC21950.16375464406678505No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT21580.16099431521463423No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT21540.16069590128467195No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG21190.15808477939750226No Hit
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC21150.15778636546754002No Hit
GCAAGTGACCCTTGTTTTGTTTTGGTTGGGGTGGGGGGTGTTTGGGATGG20400.1521911042807478No Hit
ATGATGATAGGTTTTACCCATCCACTCACAAGCCGGGGGATATTTTTGCA20300.15144506945584219No Hit
CATTTCTTTCATCCAGCCATGTTATCCCTTATTTGATCCACTCGAAGAGC20200.15069903463093656No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA19730.14719267095388014No Hit
GCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGAC19710.147043463988899No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA19630.14644663612897452No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC19630.14644663612897452No Hit
GTCATTGCCAATCATACGCATAAATTCCTAAAACAAACCCACTTTCAATA19620.14637203264648393No Hit
ACCCCATCTGCTTCTTTCACGCAGGCAAGTCCGCCCTGCACTGGGCCGCC19240.14353710031184255No Hit
GCCAAGTCCTACAGACTCCAACTTCTACCGTGCCCTGATGGATGAAGAAG18880.1408513749421823No Hit
AAACCTCTCATAATGAAGGCCCCCGCTGTGCTTGCACCTGGCATCCTCGT18740.13980692618731444No Hit
ACAGTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGT18730.13973232270482386No Hit
GCAACAGACTTTAAAGAAGTTGTGTTTTACAATGCAGAGAGTGGAGGATG18520.13816564957252203No Hit
GCTTATAAAAATGTGTGGGTAATCTGCTTACCTGTCAGCAATACGACCAA17880.13339102669312602No Hit
AAATCGTATTAAATTCTTACCAGCAGTATGTGAAGTGTCTGTCACAACAG17750.13242118142074874No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC17730.1322719744557676No Hit
GAAAACCAAAGCCTTTTTCATGTTAGATGGGATCCTTTCCAAATACTTTG17330.1292878351561451No Hit
ACTTGTTTATATTACTTTCTCCAGTTTTATAAACAGTTCAATAATTTTCT17100.12757195505886215No Hit
CTTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATT17000.1268259202339565No Hit
AGAAAATGTGTGGGAGATTGTTAAGTGTGATAAGTAAAACCCATGTACAT16850.12570686799659808No Hit
GCTCGTCCCGCCCGCAGCCCTAGGCGGCGTCGCCGCAGCCGATCCCGGAG16820.1254830575491264No Hit
AAAACAAAATGAAATGAATGGTGCTTACTTCAAGCAAAGCTCAGTGTTCA16650.12421479834678682No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC16580.12369257396935289No Hit
CACCAGGATGCGGATAAATAATGTTTTCAAGGATTTCCTAAAGGAATTTA16550.1234687635218812No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT16550.1234687635218812No Hit
ATTCTGAGAAAATTCCAAAATTGTGTCGACTCTTGTCCAAGCATCAGGAT16250.12123065904716432No Hit
CAGCCCTCACGTCTCCCCTGGCATCCCAGCCTCGCGCTCACCCTGTTGTT16140.12041002073976813No Hit
CCACATGAGATTCCAGAAGAAATGTGTGATTAAAATCCAAATTCATGCTG16130.12033541725727757No Hit
CCCAACTCCCCGCCATGGGCCCTCCCAGGGCTGCCCCTACACCAGGCGCC16030.11958938243237195No Hit
AAATAAGACTGTCCTCATGGCTCTGTGACTGTGCCTCTTGTCAGGTGTAT15970.11914176153742857No Hit
ACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCC15960.11906715805493799No Hit
AATATTAATCTTCAACCATTTCTTTCCATAATCAATTCCATAAACAGATA15820.11802270930007014No Hit
AAAAGGCAGCTTGGACACGGCTTTACCTCCAATGGTGCAGGCTCCAAGTA15750.11750048492263619No Hit
ACAGAAGGTGGTGGTGTTGCCATGGGGATGTATAATCAAGATAAGTCAAT15630.11660524313274945No Hit
GCAAGTAGTACAGATAGGTTAGCACCATGCTTTGTATTTTATTTAAAAGA15610.11645603616776831No Hit
ATTACTTTTCCAAGGACGGTTGAAGAAATTGCTTGCTTCCACTAAAAATG15500.11563539786037212No Hit
AAAACTCCACCATTCAGAGTGCTTTTTCCTTCTGAAGGAAGCTGAGGAAC15340.11444174214052312No Hit
CTCTTCCTGTCCAGGGATCACTGGAACTTGGGGCAGATACTGGTACTTTG15270.11391951776308917No Hit
GTTCTAATTGCACTGTTTTTTCTCATTCCTTCCCCAGGCTGGATGATAGA15190.11332268990316469No Hit
AAATTAATACTTTTAAAAGTCATTACTGCTAGGTTTTTAATGCTTTAATG14770.11018934363856105No Hit
CATCCAGGTTGATGGTGCAGAAGCGTCCAGCAAAGCCCTCAGGACAGAGG14700.10966711926112711No Hit
CTCCAGGGAAATCGTGCCAGCAGGCTGACCCGTGCGCCTCCAACCCCTGC14570.10869727398874979No Hit
GCAAAGGAAAAGGTCTAGGAGAATATGTAAATTTAACATTGATTAACTGA14410.1075036182689008No Hit
GACATGGCCAACTGCCCCACCCCAAGTCTGGAGAGGCCGGTGGGAGAAGC14230.10616075558407066No Hit
ACACTGGAAATTCCAACATGCCTGGGGGGCTCCCAAGGCAAGCTTACACC13950.10407185807433492No Hit
GTTTTACTAGTGGTCCTTTGGCGTGCTCCTCTGGGAGCTGATGACAAGAG13890.10362423717939152No Hit
CACCGGGCCCTGGCCAGCCTCACCTTGCCAGCCCGTGGGGCAGACACAGG13830.10317661628444816No Hit
AATGTTTTGTCAAAGTTTCCAAGGTAGCCAAGTATTTCACCTTCAGGTCA13760.10265439190701423No Hit
CAGCCAGTGGGAAGCTTTACCTGATGTCCTTGGGCAGTTCTGGCAACTGC13610.10153533966965579No Hit
GACATCAGTTTGCCAGTTGTGCTTTTTGCTAAAATGCATGTTTCCAATTT13590.10138613270467464No Hit
AATGAATGATTCAAAAGAATACATTAGCTTAAGTTTTGTTTTTTTTAATA13480.10056549439727847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)