FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002435053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340420
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT27427920.462168574029036No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA123110.9184434729413169No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC120780.9010608615210158No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC115930.8648781725130928No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT105350.7859476880380776No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC95520.7126124647498545No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT88470.6600170095940079No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG86140.6426343981737068Illumina Single End PCR Primer 1 (100% over 50bp)
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA80710.6021247071813313No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT78210.5834738365586906No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA75180.5608689813640501No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC74020.5522149773951448No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT70070.5227466018113726No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT67030.5000671431342415No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC64640.482236910818997No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC60770.4533653630951493No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC59960.4473224810134137No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA58770.43844466659703674No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC57760.43090971486548996No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG55790.41621282881484906No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA54730.4083048596708494No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA54160.4040524611688873No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC53540.3994270452544725No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG52150.38905716118828426No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT51250.38234284776413363No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA50150.37413646469017175No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT49820.3716745497679832No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA49500.36928723832828514No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT48710.3633935632115307No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG46250.3450411065188523No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT45720.3410871219468525No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT44900.33496963638262633No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG43770.32653944286119274No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA43720.32616642544873997No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT43560.32497276972889094No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA42900.32004893988451383No Hit
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA41820.31199176377553306No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC41750.31146953939809907No Hit
GGGACACCGCTGGGCCTGCATCTGACCTGTTGTGCTCACTGCTTAGGACC40650.3032631563241372No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA38590.2878948389310813No Hit
GGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGGAGCTGCACTG38390.28640276928127No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG38030.2837170439116098No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG37100.2767789200399875No Hit
GGATTCACATTAACTCTATGACCATATTTTATTCCAGACACTTCTGAGAA36690.2737201772578744No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA35720.26648363945628983No Hit
GGAAGACACCTTGTGCGTCGGCAGACACAGCCGCATGCAGCGGGGTGCGG35170.26238044791930887No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT34970.2608883782694976No Hit
GCTGCTCTTCCTCCTCTCCCCTACGTCTCTTGCTCCTAGATCTTCACGCT34880.2602169469270825No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG34550.25775503200489397No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG34250.2555169275301771No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT34240.2554423240476866No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC33660.2511153220632339No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT33130.2471613374912341No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC32910.2455200608764417No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT32090.2394025753122156No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC32050.23910416138225332No Hit
AGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGACACTCACCTGCAG31520.2351501768102535No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGCGGGGGG31400.23425493502036676No Hit
GGGTCCTGCCCTTGCACAGATGGGGTCTCATTCTGCCCCATCCCCATAGG30680.22888348428104624No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG30650.22865967383357455No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA30610.2283612599036123No Hit
GGCCACACTCGTTGACATCCTGCCGGCAGGTGGGGCCATGGAAGCTGGGT30390.2267199832888199No Hit
AGGTACTCACAAGCCTTCCCTGAAGCCATCAATCAGTGCTTGGAAGAGAG30320.22619775891138597No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG29940.22336282657674458No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA29600.22082630817206547No Hit
GGTATTGGTCAGAAGTTCAACACTGTAGCCATTGCAACATGCTCAGTTAA29470.21985646289968816No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT29020.21649930618761284No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA28680.21396278778293373No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT27800.2073976813237642No Hit
GGACAAGAACAGCAACGAGTACCGGGTGCGGCGCGAGCGCAACAACATCG27780.20724847435878305No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC27680.20650243953387742No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT27200.20292147237433045No Hit
GGGGTAACTTTTGGGTCATAATTCTTAGGCAATTTTCCTGCATAACGATT27150.20254845496187765No Hit
GCGGAAGCACCAGCTGAGAAGGTGGAGGGGACAGGATGGTACCTACCGTA26500.19769922859999103No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT26220.1956103310902553No Hit
GGACAGAGGAATTGAGAGGTCCTTCTGGGACTCTTTGAGCTTCTCAGCCA26170.19523731367780248No Hit
GAAGAGGACGAAAAACGATTTCTTGTTACTGTTATAAAGGTATGCAAGGG26070.19449127885289685No Hit
GGATTCTGTTGTTAGCTGATAAGAGTAACACAGCCAGAGCTGATTCAGAC25910.19329762313304785No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT25800.19247698482565168No Hit
GCAGGAGATGATCCGGCAACTGGAACAGACAAACTATCGACTGAAGTTGT25740.19202936393070827No Hit
GAGATCACTCACCCATCGCATACCTCAGGGCAGATCAATTCCGCACAGAC25480.19008967338595364No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC25250.18837379328867074No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC25090.1871801375688217No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC25000.18650870622640664No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG24920.18591187836648215No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT24720.1844198087166709No Hit
GAGGGACCAGGGTTGGAAATAGAATGCGGGAAGGAGCAGCTTGGCTAAAC24580.18337535996180301No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA24480.1826293251368974No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA24270.1810626520045956No Hit
AGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACACCCAGCT24110.17986899628474656No Hit
GAGAAATCAATGTAAACACCATCTTACCAATGGGATTGGTGGACGTCTCC23650.1764372360901807No Hit
AGCCGCCCAGGTAGTCCGTGCAGGTGGCCCCGTTCTGGCAGGGGCTGGGT23610.17613882216021842No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA23380.17442294206293552No Hit
GTAGAAGACTCTTATGAGCGAGATAATGCAGAGAAGGCCTTTCATATAAA23060.17203563062323748No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC22760.16979752614852062No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG21870.16315781620686054No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA21480.16024828038972858No Hit
ACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCC21340.1592038316348607No Hit
GAGAAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGGAAATCACCAAA21190.15808477939750226No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA21150.15778636546754002No Hit
GTCATTGCCAATCATACGCATAAATTCCTAAAACAAACCCACTTTCAATA21000.15666731323018157No Hit
GAAAACCAAAGCCTTTTTCATGTTAGATGGGATCCTTTCCAAATACTTTG20990.15659270974769102No Hit
AACGGTGTTACGGCATAAATGTAGTCTTTTCCCATTTCACCAATATATTC20980.15651810626520046No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG20980.15651810626520046No Hit
GCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGAC20600.15368317393055908No Hit
ACAGTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGT20130.15017681025350263No Hit
GAGAATCACAATCACTGCAGCCTCACAGGTGTGGCCAGCTCGCTCCCGCA19970.14898315453365363No Hit
AGTGTTCATTTAATTTTGGTTTATTTTCTCCTTTACAGATCTATATGATC19880.14831172319123856No Hit
GCTGAACTTAAACTTACCAGATTACATTATAATGCATTTTTTAATTTTCA19410.1448053595141821No Hit
GGTGCCTACCATGGTCAATGGGGTAGACCTTCAGCCCATCCAGCTCAAAG19000.14174661673206906No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC18910.141075185389654No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC18730.13973232270482386No Hit
GGAGCAGAATCCCGACCTCAAAGACGCCCCTTGCCTTGTACACCAGGCGA18730.13973232270482386No Hit
GTAGTATTTCTCCATTACCTAGGCTGAGAATTCTCCTCATACATTCTTTC18660.13921009832738993No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG18330.13674818340520134No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG17970.1340624580355411Illumina Single End PCR Primer 1 (98% over 50bp)
CAGCCCTCACGTCTCCCCTGGCATCCCAGCCTCGCGCTCACCCTGTTGTT17960.13398785455305054No Hit
AGAGTGGAAGGCCGAGAGATCATTTCTAATTTGGCAAAGGTATTTACATT17910.13361483714059771No Hit
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC17890.13346563017561658No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG17890.13346563017561658No Hit
GTGCCTGCCTGCCAGGTAGTGCTGCCCGCTGGCCGGGGTGCACGAGCCCC17600.1313021291833903No Hit
GATGTTATCCGTGCGCAGGATCTGCTTCTTGAGGTGGCTCATGAGGTCTC17300.1290640247086734No Hit
GGACACAGACACAACAGGCACAGCTTTGAAGGCTAGAATGAAAAGAAATG17120.1277211620238433No Hit
ACCCCATCTGCTTCTTTCACGCAGGCAAGTCCGCCCTGCACTGGGCCGCC17110.1276465585413527No Hit
AATGTTTTGTCAAAGTTTCCAAGGTAGCCAAGTATTTCACCTTCAGGTCA16630.1240655913818057No Hit
AGAGGCAGATACTGTTGCAGCACTGCCAGGTCTTGGGGAGGTGGGTGGGC16580.12369257396935289No Hit
GGTATTCTATGTCTTTTAGGCTTACAGATACTTTTTACACTCTTACAAAA16540.12339416003939063No Hit
AGTGTTTTTGGTGCTCAGATACAAAGGCCAACCCTTAGACAGAGCCATTT16470.12287193566195671No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT16350.12197669387206994No Hit
GGAAGAGCTTTCGACAGGCTCTACATGAAGTGCATCCCATCAGTTCTGGG16330.12182748690708883No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA16310.12167827994210768No Hit
GCTTATAAAAATGTGTGGGTAATCTGCTTACCTGTCAGCAATACGACCAA16210.12093224511720206No Hit
GGTGAGAATTTGGGGCCTGGAAAAATGAAGTCATTGGAAGATAAGATTCA16100.12011160680980587No Hit
GAACACTCGGCCGGCAGAAGGCTGCCACATGCAACTCAGGAACTCACTGC16000.11936557198490025No Hit
GACATCAGTTTGCCAGTTGTGCTTTTTGCTAAAATGCATGTTTCCAATTT15980.11921636501991914No Hit
GAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCCAACCCCACTCCCA15640.11667984661524No Hit
GAAGGCAGTTATGCCATTTGTAGAATGGTAATAACCAGTTGGTATTTGGG15370.1146655525879948No Hit
GGGAGATCCCTCTGGGTGAGGCTCCCGGGGTCACGTGTGTCTCCTTCCCG15240.1136957073156175No Hit
AAAACTCCACCATTCAGAGTGCTTTTTCCTTCTGAAGGAAGCTGAGGAAC15170.11317348293818355No Hit
AAACCTCTCATAATGAAGGCCCCCGCTGTGCTTGCACCTGGCATCCTCGT15100.11265125856074962No Hit
AGGTGACATTTTAATTTACAAATTGGCATCATTTTGGTCGACAAATACCC15070.11242744811327793No Hit
GCAGAAGCTGCACTTTATAAGAATCTTCTGCATTCAAAGGAGTCTTCCTG14910.11123379239342893No Hit
GGGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGG14900.11115918891093837No Hit
GAGAGAGAGAGAGAGACAGAGATTTCATACCAGAGAGCTCAGGAGGGGAG14820.11056236105101386No Hit
AATGAATGATTCAAAAGAATACATTAGCTTAAGTTTTGTTTTTTTTAATA14480.10802584264633472No Hit
ATTCTGAGAAAATTCCAAAATTGTGTCGACTCTTGTCCAAGCATCAGGAT14440.10772742871637249No Hit
AGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAGACCCCAAG14370.10720520433893854No Hit
GAGGTAAATGTGGATGACTGCCTGATGCGGCCTTGTGCTAACGGTGCCAC14350.10705599737395742No Hit
AAAACAAAATGAAATGAATGGTGCTTACTTCAAGCAAAGCTCAGTGTTCA14270.10645916951403292No Hit
GAGGATCAAGAGCGTCATTTACTTGTGAAAGTTATTGATAGGATACTGTA14190.1058623416541084No Hit
CGTGCGGATCTGGACTTGGAGGTCGACCGAGATCGAGAACGAGTGCGGGA14090.10511630682920278No Hit
AAATAAGACTGTCCTCATGGCTCTGTGACTGTGCCTCTTGTCAGGTGTAT14070.10496709986422166No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGAT13990.10437027200429715No Hit
GGCAACGAGAAATATATTGGTGGAGAACGAGAACAGAGTTAAAATTGGAG13820.10310201280195759No Hit
GGAAAATGCTTTACCTTAGAAGCAGTTCCAAGAGCAGAAGCACTTGAGGG13770.10272899538950478No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAT13690.10213216752958028No Hit
GCACCTGGTGTGAGGGTTTTTGGAACTGGAGATGTTGGTCCACTGTACCT13680.10205756404708971No Hit
AAAGATCTGCTGCCTAGACTCACCCCCATCTTAAAGAACAGACATGAAAA13630.1016845466346369No Hit
CACCAGGATGCGGATAAATAATGTTTTCAAGGATTTCCTAAAGGAATTTA13540.10101311529222183No Hit
GACATGGCCAACTGCCCCACCCCAAGTCTGGAGAGGCCGGTGGGAGAAGC13530.10093851180973128No Hit
GACTATTACTGAGCGGAATATTGATACTTACAGGGCAGGATGTTTTTATA13450.10034168394980676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)