FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002435056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873503
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC36436719.44843429660908No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1103865.891957472179121TruSeq Adapter, Index 12 (100% over 50bp)
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA135680.7242048718363407No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG131920.7041355151286119No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA98870.5277280047056236No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC94540.5046162189225211No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC89820.47942277114047854No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC85640.4571116245877375No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC84330.4501193753092469No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG77830.4154250086602477No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT76390.40773887204877707No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT76260.4070449847157971No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC74210.39610291523418967No Hit
ACACACACACAAACACACACACACACACACACACACACACACACACACAC72080.38473383816305606No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG71130.37966312303743305No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC70890.37838210026885466No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC69590.37144322693905485No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC69360.37021558011916716No Hit
GATCGGAAGAACACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC67090.3580992397663628TruSeq Adapter, Index 12 (98% over 50bp)
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC64990.34689029054130155No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA60410.32244410604092977No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC60230.32148333896449593TruSeq Adapter, Index 12 (98% over 50bp)
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG57740.30819272774049467No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG57740.30819272774049467No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC54150.28903076216050894No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC53290.2844404305731029No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC48820.2605813815083296No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG46770.24963931202672215No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT46140.24627662725920374No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT45770.24430171715764532No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT44840.2393377539294039No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG43120.2301570907545918No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT41710.2226310819891935No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA41040.21905489342691203No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT40560.21649284788975517No Hit
GCCACGCCACTCACCTGTGGCTGGCTTCCGGAAGCCTCTCAGGAGCGGGG40520.2162793440949921No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA40440.21585233650546595No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG40240.2147848175316506No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG40000.21350379476307216No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG39380.21019448594424456No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA38550.20576428220291082No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC38050.20309548476837241No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA37700.20122732656419554No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG37240.1987720329244202No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT37180.19845177723227558No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC37050.19775788989929557No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC35310.18847047482710197No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT34350.18334638375278822No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG34130.18217211288159132No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA33860.18073096226694058No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC33710.17993032303657905No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC33540.179022931908836No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC33310.17779528508894835No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT33130.1768345180125145No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG32840.17528661550048227No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA32620.17411234462928535No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA32480.1733650813476146No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG32040.17101653960522079No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC31920.1703760282209316No Hit
GATTGAAGAAGACGTCCACCTCGTCTGGAGGCAGCAGCTGCGCGGGTTCC31470.16797411052984704No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA31130.16615932827436092No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA30990.16541206499269015No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC30860.1647181776597102No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA30710.16391753842934867No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT30670.16370403463458558No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT30650.16359728273720406No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG30390.1622095080712441No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA30350.161996004276481No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA30160.1609818612513564No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG29470.15729892079169341No Hit
GAACAGTGCATGGGCCTATCAGGTTCAGTTTTCTCCACTGGGCCAGCTCC29080.15521725879275347No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC28950.15452337145977346No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC28740.15340247653726735No Hit
GTCCCCACGCGGAAGGCAACTGCCTGAGAGGCGCGGCGTCGCACCGCCCA28440.15180119807654432No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC28420.15169444617916278TruSeq Adapter, Index 12 (98% over 50bp)
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG28330.15121406264094586No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT28280.15094718289749204No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT27710.14790475382211826No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG27650.14758449812997365No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC27440.1464636032074675No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA27390.14619672346401366No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG27260.14550283613103368No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT27010.14416843741376448No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA26960.14390155767031063No Hit
GATTTCTCTAATAGCTGCCACATTAGGACCTGCTCCTAGATGGGTATAAA26950.14384818172161987No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC26800.14304754249125834No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT26530.14160639187660762No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT26440.1411260083383907No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA26310.14043212100541072No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG26280.14027199315933842No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA26160.1396314817750492No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT26080.13920447418552306No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG25810.13776332357087231No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC25080.13386687931644625No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA25060.1337601274190647No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT24970.1332797438808478No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG24550.13103795403583554No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG24520.13087782618976324No Hit
CCACAGCTGTGCCCAGTGGGGCACTGGGGCCGCGCGTGCCCCCCTCACTG24420.13034406670285556No Hit
ATCCTCCGTGGTCATGCTCCAATAAATTCACTGCTTTGTGGCGCGACCCT24410.1302906907541648No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG24380.1301305629080925No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC24360.13002381101071095No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG24310.12975693126725713No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA24110.12868941229344175No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG23900.12756851737093564No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT23750.1267678781405741No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT23620.12607399080759413No Hit
GACAGATAACGTACCTCTTCCACTTCGACCGACAAACCTGAGGTCATTAA23250.12409908070603569No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC23080.12319168957829263No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA23010.12281805793745726No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG22920.12233767439924034No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA22720.12127015542542499No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT22650.12089652378458962No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA22590.12057626809244501No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC22580.12052289214375424No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG22460.11988238075946502No Hit
AAGCAGTGAGCACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTC22330.11918849342648505No Hit
CACCTGACTGTTTTCTCTTTTCCACGTTGAGTACACAGAACTAGACACAT22010.11748046306838046No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC21890.11683995168409124No Hit
ACCACCCCATCCCCCGCAGGTGTTGACGTTGCCCGCCTGTGCCAGCATGG21860.11667982383801895No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG21710.11587918460765742No Hit
GTCTGAGGCCAGCAAGAAGAAGCGGCGGGAGCCCCTCGGCGAGGACTCCG21560.1150785453772959No Hit
ATACAAAGAGCTAAATAGGCGAATTGATCTAAAACAGATACATTCAAATT21380.11411777830086207No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA21330.11385089855740824No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC21260.11347726691657285No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT21250.11342389096788208No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT21100.11262325173752057No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA20670.11032808594381754No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG20650.11022133404643601No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT20530.10958082266214679No Hit
ATCCAGGTTTCTGTTGTTACAGTCTCTCTTCTGCCTCCTAGGCCGTTGGC20440.10910043912392987No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT20400.1088869353291668No Hit
ACTTACATACAGTTTCTTGCAGCCAAGTCTCTGTGGACAAACTTTTTGCT20400.1088869353291668No Hit
GCAAGAGGGAAAACATGGCACGGGTAGGTCATTAGCCACACTAATTTAAC20390.10883355938047604No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG20310.10840655179094989No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG20250.1080862960988053No Hit
GTCCTCTTCACCCTCACATTGCTCCCCAGGTGGCAGTTTTGGCACAGGAA20130.10744578471451607No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT19940.10643164168939148No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG19880.10611138599724687No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC19640.10483036322866844No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG19630.10477698727997767No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA19490.10402972399830691No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA19300.10301558097318234No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG19210.1025351974349654No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT19170.10232169364020234No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA19150.10221494174282081No Hit
GCAAGTGACCCTTGTTTTGTTTTGGTTGGGGTGGGGGGTGTTTGGGATGG19110.10200143794805773No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC19070.10178793415329467No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT19050.1016811822559131No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA18990.10136092656376852No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC18970.10125417466638696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)