FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002435060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350842
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC7774022.158122459682705No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3874911.044572770648896TruSeq Adapter, Index 9 (100% over 50bp)
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA26390.7521904447016036No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC25660.7313833577507824No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG25580.7291031290438431No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC24560.7000302130303669No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC23300.6641166108960729TruSeq Adapter, Index 9 (98% over 50bp)
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA23300.6641166108960729No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC20560.5860187776834016No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA19720.562076376260539No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT17090.48711385751990927No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC17090.48711385751990927No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT16600.473147456689906No Hit
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC16450.46887202786439475No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG16030.45690082715296343No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT15800.45034516962051296No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA15730.448349969501941No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA15320.43666379737887717No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT15020.4281129397278547No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC14340.40873099571887056No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC13960.39789990936090885No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA13410.38222333700070116No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG12390.35315042098722504No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC12290.3503001351035509TruSeq Adapter, Index 9 (98% over 50bp)
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC12130.3457396776896723No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG12040.3431744203943655No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG11640.331773276859669No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA11430.32578767650395335No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG11370.3240775049737489No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT11340.32322241920864664No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT11300.32208230485517697No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC10530.3001351035508862No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT10510.2995650463741513No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA10350.2950045889602727No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG10320.29414950319517047No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA9980.28445853119067843No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG9830.2801831023651672No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC9530.2716322447141448No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG9480.2702071017723078No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA9470.26992207318394035No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT9450.2693520160072055No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT9170.2613712155329179No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC9020.25709578670740674No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG8940.25481555800046746No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC8870.25282035788189555No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT8720.24854492905638437No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG8480.24170424293556642No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA8440.2405641285820968No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA8260.23543361399148335No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG8110.23115818516597214No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT8070.2300180708125025No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG8050.22944801363576767No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA7820.22289235610331715No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT7810.22260732751494977No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC7750.22089715598474527No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA7610.2169067557476015No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC7560.21548161280576442No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG7460.21263132692209027No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG7450.2123462983337229No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT7390.2106361268035184No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT7330.20892595527331392No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT7220.20579064080127238No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC7140.20351041209433307No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA6950.19809486891535222No Hit
ACACACACACACACACACACACACACACACACACACACACACACACAGAT6620.18868892549922756No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT6580.18754881114575794No Hit
CAACACCTACGAAGGAAACATCATGAAGAATAAAGAGAAACTGCAGAGCC6520.18583863961555344No Hit
AAACTTTCTCAAATAAATTCATTTCCATTTTAATGTTATTTATTCCCAGG6500.1852685824388186No Hit
GCAACAGACTTTAAAGAAGTTGTGTTTTACAATGCAGAGAGTGGAGGATG6450.18384343949698154No Hit
GTTCGATATTCATCACGGCGCGCTTCACAGCCTGATGAATTTCTCAGAAG6430.1832733823202467No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG6420.1829883537318793No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA6410.1827033251435119No Hit
CACCTGGCCCGTTCCCTTGCTTCACATCTTCTCGGGCCCCACAGGTATAA6210.1770027533761636No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT6170.17586263902269397No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA6030.1718722387855502No Hit
GTCCCTGGACAGCAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGG5980.17044709584371315No Hit
AACGGTGTTACGGCATAAATGTAGTCTTTTCCCATTTCACCAATATATTC5930.16902195290187605No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT5880.16759680996003898No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA5830.1661716670182019No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA5820.1658866384298345No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT5670.16161120960432332No Hit
GCAAAGGAAAAGGTCTAGGAGAATATGTAAATTTAACATTGATTAACTGA5660.1613261810159559No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC5620.16018606666248625No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA5590.159330980897384No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG5570.15876092372064918No Hit
GCAAGTAGTACAGATAGGTTAGCACCATGCTTTGTATTTTATTTAAAAGA5460.15562560924860763No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG5440.1550555520718728No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT5370.15306035195330092No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC5300.15106515183472902No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA5300.15106515183472902No Hit
ATCTGGACCCCAGGGCAGGGCTTCATGCATCCACGCACCCACCCCGTGGC5270.15021006606962678No Hit
CATTTCTTTCATCCAGCCATGTTATCCCTTATTTGATCCACTCGAAGAGC5260.14992503748125938No Hit
CCACAGCTGTGCCCAGTGGGGCACTGGGGCCGCGCGTGCCCCCCTCACTG5200.1482148659510549No Hit
CATTTTCTGACTCCTCGGGACCAGTGTTGGCATGGGCTGCGGCCAAATTC5200.1482148659510549No Hit
GAAAACCAAAGCCTTTTTCATGTTAGATGGGATCCTTTCCAAATACTTTG5190.1479298373626875No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT5150.14678972300921783No Hit
GAAAGGAACAGGTATTTAGCATTGCAGCTAGTTTACTGGCATTATCAGCA5130.146219665832483No Hit
ACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCC5130.146219665832483No Hit
ACAGTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGT4870.13880892253493024No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC4830.1376688081814606No Hit
ATTCTGAGAAAATTCCAAAATTGTGTCGACTCTTGTCCAAGCATCAGGAT4770.13595863665125613No Hit
CCACAAGAGGTCCCAAACCCCCCAGTACCTGTGTGGTTGCCCTCAGAGGC4630.13196823641411232No Hit
AAATTAATACTTTTAAAAGTCATTACTGCTAGGTTTTTAATGCTTTAATG4580.13054309347227527No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA4530.1291179505304382No Hit
GCAAGTGACCCTTGTTTTGTTTTGGTTGGGGTGGGGGGTGTTTGGGATGG4520.1288329219420708No Hit
ATGATGATAGGTTTTACCCATCCACTCACAAGCCGGGGGATATTTTTGCA4520.1288329219420708No Hit
GCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGAC4470.12740777900023373No Hit
ACTTGTTTATATTACTTTCTCCAGTTTTATAAACAGTTCAATAATTTTCT4450.1268377218234989No Hit
AATAATAATACTGCCTACCTCTTAATGAAAAGGCACCCTAGAAGAAGAAC4440.12655269323513146No Hit
ATTACTTTTCCAAGGACGGTTGAAGAAATTGCTTGCTTCCACTAAAAATG4440.12655269323513146No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC4380.12484252170492702No Hit
AAATCGTATTAAATTCTTACCAGCAGTATGTGAAGTGTCTGTCACAACAG4370.1245574931165596No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC4310.12284732158635511No Hit
AAACCTCTCATAATGAAGGCCCCCGCTGTGCTTGCACCTGGCATCCTCGT4270.12170720723288546No Hit
ACACACATTTCCATGGACCAGGTCTTTATCCTCCTGCAGAGTAAAAAGGT4210.11999703570268098No Hit
ACACACACACACACACACACACACACACACACACACACACACACAGATCG4200.11971200711431357No Hit
GACTATTCTTGGTTTCACAGCTGATACCCAACTCTTGTGCAACTCATGCC4170.11885692134921133No Hit
CCTCCCAAGGCTTCAGCTCTGACAGCAGAGGGTGGAAGCTGCCTCATCTC4170.11885692134921133No Hit
GTTCTAATTGCACTGTTTTTTCTCATTCCTTCCCCAGGCTGGATGATAGA4100.11686172123063943No Hit
CTTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATT4070.11600663546553719No Hit
AATATTAATCTTCAACCATTTCTTTCCATAATCAATTCCATAAACAGATA4070.11600663546553719No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA3970.11315634958186306No Hit
AGAAAATGTGTGGGAGATTGTTAAGTGTGATAAGTAAAACCCATGTACAT3940.11230126381676082No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC3850.1097360065214541No Hit
AAAAGGCAGCTTGGACACGGCTTTACCTCCAATGGTGCAGGCTCCAAGTA3820.10888092075635188No Hit
AATGAATGATTCAAAAGAATACATTAGCTTAAGTTTTGTTTTTTTTAATA3810.10859589216798446No Hit
GCTTATAAAAATGTGTGGGTAATCTGCTTACCTGTCAGCAATACGACCAA3810.10859589216798446No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC3720.10603063487267773No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC3650.10403543475410583No Hit
AAATAAGACTGTCCTCATGGCTCTGTGACTGTGCCTCTTGTCAGGTGTAT3640.10375040616573841No Hit
GACATCAGTTTGCCAGTTGTGCTTTTTGCTAAAATGCATGTTTCCAATTT3640.10375040616573841No Hit
CTCAATTGTCTCCTCTACTTTAGATCGGAAGAGCACACGTCTGAACTCCA3640.10375040616573841Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACTGGAAATTCCAACATGCCTGGGGGGCTCCCAAGGCAAGCTTACACC3640.10375040616573841No Hit
GAACACTCGGCCGGCAGAAGGCTGCCACATGCAACTCAGGAACTCACTGC3630.10346537757737102No Hit
AATAATTTCACAGAAGTCAAATGCAATTTTAGAAGATACTCTACAAAGTT3620.1031803489890036No Hit
CTCTTCCTGTCCAGGGATCACTGGAACTTGGGGCAGATACTGGTACTTTG3600.10261029181226877No Hit
CCACATGAGATTCCAGAAGAAATGTGTGATTAAAATCCAAATTCATGCTG3540.1009001202820643No Hit
GTTTTACTAGTGGTCCTTTGGCGTGCTCCTCTGGGAGCTGATGACAAGAG3510.10004503451696205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)