FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002435061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350842
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT7451221.238050176432697No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG42751.2184972152706917Illumina Single End PCR Primer 1 (100% over 50bp)
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA27160.7741376460058944No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT26010.7413593583436419No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC26010.7413593583436419No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC25380.7234025572764948No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA23550.6712423256052582No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT20840.5939995781576892No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA20460.5831684917997275No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT18680.5324334030703279No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC17790.5070658587056281No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC17520.49937008681970807No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT17300.49309945787562487No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA16340.4657367133923533No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC16300.4645965990388836No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC16020.456615798564596No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC14830.42269739654887384No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC14590.41585671042805594No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC14530.41414653889785147No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT14240.4058807098351965No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT13770.39248436618192806No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA12890.3674018504055957No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA12780.3642665359335541No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC12280.35001510651518347No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA12150.3463097348664071No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG11570.3297780767410971No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA11550.3292080195643623No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG11070.31552664732272645No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG11020.3141015043808894No Hit
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA10990.31324641861578717No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA10660.3038404751996625No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT10620.3027003608461929No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA9980.28445853119067843No Hit
GGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGGAGCTGCACTG9970.284173502602311No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA9870.28132321671863686No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG9670.27562264495128863No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT9610.27391247342108416No Hit
GAAGAGGACGAAAAACGATTTCTTGTTACTGTTATAAAGGTATGCAAGGG9400.26792687306536844No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT9290.2647915585933269No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT9250.26365144423985726No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT9150.2608011583561831No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT9040.2576658438841416No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC8710.24825990046801694No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG8690.2476898432912821Illumina Single End PCR Primer 1 (98% over 50bp)
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA8590.244839557407608No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT8540.24341441446577092No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA8410.23970904281699454No Hit
GCTGCTCTTCCTCCTCTCCCCTACGTCTCTTGCTCCTAGATCTTCACGCT8390.2391389856402597No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG8380.23885395705189233No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG7970.22716778492882836No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT7960.22688275634046096No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG7630.2174768129243363No Hit
AGGTACTCACAAGCCTTCCCTGAAGCCATCAATCAGTGCTTGGAAGAGAG7600.21662172715923408No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA7510.21405646986392735No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC7340.2092109838616813No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT7300.20807086950821166No Hit
GCTCAAGTAAAGCTCTTTGTGACAGGGCTTTTCAGCTTAAATCAAGATAT7200.20522058362453754No Hit
ATTTGATAGGGAATGCACACATGGCAGATCGATCCATTGGTTCGGCAGAA7100.2023702977408634No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG6970.19866492609208705No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT6870.1958146402084129No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC6840.19495955444331067No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC6810.1941044686782084No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT6800.193819440089841No Hit
GCTGAACTTAAACTTACCAGATTACATTATAATGCATTTTTTAATTTTCA6710.1912541827945343No Hit
GGTATTGGTCAGAAGTTCAACACTGTAGCCATTGCAACATGCTCAGTTAA6690.19068412561779946No Hit
GGATTCACATTAACTCTATGACCATATTTTATTCCAGACACTTCTGAGAA6670.19011406844106463No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG6530.18612366820392084No Hit
GGACAGAGGAATTGAGAGGTCCTTCTGGGACTCTTTGAGCTTCTCAGCCA6500.1852685824388186No Hit
ACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCC6460.18412846808534897No Hit
GGGGTAACTTTTGGGTCATAATTCTTAGGCAATTTTCCTGCATAACGATT6180.1761476676110614No Hit
GAAACTAATATATTTTACCTTGAATTAAAAAATATTTAAAGTTCGTACAT6020.17158721019718276No Hit
GGGTCCTGCCCTTGCACAGATGGGGTCTCATTCTGCCCCATCCCCATAGG5980.17044709584371315No Hit
GAGATCACTCACCCATCGCATACCTCAGGGCAGATCAATTCCGCACAGAC5950.16959201007861088No Hit
AATATTTCTGCAAGAGCAAATAAATACATACCTTTATTTGAACTAGGAAA5920.16873692431350865No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG5920.16873692431350865No Hit
GGACACAGACACAACAGGCACAGCTTTGAAGGCTAGAATGAAAAGAAATG5880.16759680996003898No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT5860.16702675278330417No Hit
ACAAAAGCACACATATACTAAATTTTTTCCCATTGACTGGAGGAAATTGA5790.1650315526647323No Hit
GAGAAATCAATGTAAACACCATCTTACCAATGGGATTGGTGGACGTCTCC5760.16417646689963003No Hit
GACTATGGCAAGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGC5730.1633213811345278No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAT5670.16161120960432332No Hit
GAGGATCAAGAGCGTCATTTACTTGTGAAAGTTATTGATAGGATACTGTA5660.1613261810159559No Hit
GGAACTCATTGATCTTGTCCAGGCAGCGGCCATTGTGCAGGCAGGGGCTG5630.16047109525085365No Hit
GAAAACCAAAGCCTTTTTCATGTTAGATGGGATCCTTTCCAAATACTTTG5620.16018606666248625No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGAT5560.15847589513228177No Hit
AAGAAAGTTGTTTTTCAACACTTTTCTTTACTTTCCAGAGTATGAATAGC5550.15819086654391437No Hit
GTACAAAATATAGCTTTAGCAGACTTACCTGGCAGCCAGAGAATAAATGG5530.15762080936717954No Hit
GCAGGAGATGATCCGGCAACTGGAACAGACAAACTATCGACTGAAGTTGT5340.15220526618819868No Hit
GTTTAAAGCATTTCTTACCTTAATTTCCAGTACATTTTCATTGCCCATTG5310.15135018042309645No Hit
AACGGTGTTACGGCATAAATGTAGTCTTTTCCCATTTCACCAATATATTC5240.14935498030452454No Hit
ACAGTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGT5230.1490699517161571No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCGTAAT5210.1484998945394223No Hit
ATAATGGTTGCAAAAATCTCCTCACCTGAAGGACCACTTCCAGGTTCTTT5200.1482148659510549No Hit
AGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACACCCAGCT5160.14707475159758523No Hit
GTATATTATCTCCTGGAGAGACAGCTAGGCAGCAAAAAAACAATCTATTA5150.14678972300921783No Hit
AGTGTTCATTTAATTTTGGTTTATTTTCTCCTTTACAGATCTATATGATC5040.14365440853717626No Hit
ATTGAAATGTATTAGAAGATGAAGTGCGTCAGAAGAAGATAACATCACTC5040.14365440853717626No Hit
GGTGAGAATTTGGGGCCTGGAAAAATGAAGTCATTGGAAGATAAGATTCA4930.14051909406513474No Hit
GGATTCTGTTGTTAGCTGATAAGAGTAACACAGCCAGAGCTGATTCAGAC4830.1376688081814606No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT4790.13652869382799096No Hit
GATGTTATCCGTGCGCAGGATCTGCTTCTTGAGGTGGCTCATGAGGTCTC4700.13396343653268422No Hit
GTAGAAGACTCTTATGAGCGAGATAATGCAGAGAAGGCCTTTCATATAAA4680.1333933793559494No Hit
GGAAGACACCTTGTGCGTCGGCAGACACAGCCGCATGCAGCGGGGTGCGG4650.13253829359084715No Hit
ACATAACCATGCAAATGTTTTTCCTGTGCCTGACCAGGGAGGAAGTCACA4620.1316832078257449No Hit
GAGAAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGGAAATCACCAAA4610.1313981792373775No Hit
GAAGGCAGTTATGCCATTTGTAGAATGGTAATAACCAGTTGGTATTTGGG4590.1308281220606427No Hit
AGAGTGGAAGGCCGAGAGATCATTTCTAATTTGGCAAAGGTATTTACATT4560.12997303629554044No Hit
GTAGTATTTCTCCATTACCTAGGCTGAGAATTCTCCTCATACATTCTTTC4540.1294029791188056No Hit
GGAGCAGAATCCCGACCTCAAAGACGCCCCTTGCCTTGTACACCAGGCGA4520.1288329219420708No Hit
GGCCACACTCGTTGACATCCTGCCGGCAGGTGGGGCCATGGAAGCTGGGT4500.12826286476533597No Hit
GAACACTCGGCCGGCAGAAGGCTGCCACATGCAACTCAGGAACTCACTGC4470.12740777900023373No Hit
AGCTCTCTCATTTTTTTTCATTTTTTCCTTTTATAGGGTGTTGAAGATGC4470.12740777900023373No Hit
GACTCACCTGCATCTCCAGGCAGGTCCACAGTCACAGGGAGGGACCAGGA4360.12427246452819218No Hit
GAGGGACCAGGGTTGGAAATAGAATGCGGGAAGGAGCAGCTTGGCTAAAC4340.12370240735145735No Hit
GAGAATCACAATCACTGCAGCCTCACAGGTGTGGCCAGCTCGCTCCCGCA4300.1225622929979877No Hit
GGTATTCTATGTCTTTTAGGCTTACAGATACTTTTTACACTCTTACAAAA4300.1225622929979877No Hit
GGGACACCGCTGGGCCTGCATCTGACCTGTTGTGCTCACTGCTTAGGACC4250.12113715005615062No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG4250.12113715005615062No Hit
CCAGAAGCAGGCCAACTACCTCTTGTGCGCTCATCAATAATCTCCTTGAC4210.11999703570268098No Hit
GGTGCCTACCATGGTCAATGGGGTAGACCTTCAGCCCATCCAGCTCAAAG4160.11857189276084391No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG4160.11857189276084391Illumina Single End PCR Primer 1 (98% over 50bp)
AATGAATGATTCAAAAGAATACATTAGCTTAAGTTTTGTTTTTTTTAATA4160.11857189276084391No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG4140.1180018355841091Illumina Single End PCR Primer 1 (96% over 29bp)
GCTTTCCTCCTCCAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGAC4110.11714674981900684No Hit
AGAGGCAGATACTGTTGCAGCACTGCCAGGTCTTGGGGAGGTGGGTGGGC4070.11600663546553719No Hit
ATTCTGAGAAAATTCCAAAATTGTGTCGACTCTTGTCCAAGCATCAGGAT4060.11572160687716977No Hit
GGGACACCGATGGGCCTGCATCTGACCTGTTGTGCTCACTGCTTAGGACC4030.11486652111206754No Hit
GACATCAGTTTGCCAGTTGTGCTTTTTGCTAAAATGCATGTTTCCAATTT3910.11144617805165857No Hit
GAGAGAGAGAGAGAGACAGAGATTTCATACCAGAGAGCTCAGGAGGGGAG3850.1097360065214541No Hit
GAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCCAACCCCACTCCCA3760.10717074922614739No Hit
GAGCTGTGCTACAACACTGCATGTGCACTGATAGGCCAAGGCCAGCTGAA3700.1054605776959429No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGCGGGGGG3670.10460549193084065No Hit
CACCTTGTTACATCACCACACATGGCAATCCCATACAACCCTGAGTCCCA3640.10375040616573841No Hit
AAAGTAGAGGAGACAATTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGA3620.1031803489890036Illumina Single End PCR Primer 1 (100% over 29bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGATCG3570.10175520604716654No Hit
GGGAACCTGGCGGCCCACGTGCAGGGGTGGCAGAAAGGAAAAGTGGGGGG3540.1009001202820643No Hit
GCTTATAAAAATGTGTGGGTAATCTGCTTACCTGTCAGCAATACGACCAA3540.1009001202820643No Hit
GGGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGG3530.10061509169369688No Hit
AGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAGACCCCAAG3520.10033006310532946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)