FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002435153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072749
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT28209526.296458910705113No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG99880.9310658877332909Illumina Single End PCR Primer 1 (100% over 50bp)
ACACACACACACACACACACACACACACACACACACACACACACACACAC76320.7114432173789023No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA73890.6887911338066965No Hit
GGGGCCACACTTACTCTGCACGGCCTCGATCTTGTAGGGGATGTTGAGGC62900.586344056251742No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT44270.41267808219816565No Hit
GCTGCTCCATGGTCTCAATGATGCTCTTTTGCTGCACATTATCACAGCTT42120.39263611525156394No Hit
GTGTGCCTCCCACAGCATGACCTACCATCATTGGAAAGCAGTTTGTAGTC41660.3883480665095004No Hit
GGGACCCGAGCCGGGTGGGCACAGCAGGTTACCTTGTCGCTGCGCAGCCA40330.37595001253788163No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC39550.36867897336655636No Hit
GGAGGGGTGACCCAAACACTGATTCTGCTCAGACATCGTCGTGCACAAGC39030.3638316139190062No Hit
GGGACAGGGCGCTGGCAGCAAGGCTACTGTGCAGCGGGCCTACCATGCCA37220.34695907430349504No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC31560.2941974310859297No Hit
GCTGTCACTGCCTCCTCGAGATGGCACAGTCCAGAGTGAAGTAAGGCTGT30890.28795179487466316No Hit
GGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGT30650.2857145520527169No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA30380.28319765387802737No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC30250.2819858140161398No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC29450.2745283379429857No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG28870.269121667789949No Hit
GGGCTTGATGAGTGGTCGGTTCTGCCCATTCATCTTGTGGTAGAGGCCAC28850.26893523088812016No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT28660.26716408032074607No Hit
GAACTGGTGTCCCGGATATCAGCAATCTTCTCTACTTTCATTGGGGAGCA28620.2667912065170884No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA28000.2610116625603939No Hit
GGACAGGAAGAGAATTAACCAACCGGCACTCACTTCCATTTTCATTGCCC27290.2543931525454696No Hit
GGGGAGGGTCCCCACGCCTGGCCTGGCCCCCCTCACCGGCCCCCGCCCTC26330.2454441812576847No Hit
AGCGCTGCCACGTCGGGGCGGGGAAGAGCGTCGTCGCGTCCGGGTGACGT25880.2412493509665355No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG24240.22596152501656958No Hit
GGTAGCCTTAGAAAGCTGGGCTGACCTAAGGGCAGAGTTGGTGTTCTGCA22400.2088093300483151No Hit
GTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTG21950.20461449975716595No Hit
GGACATATTGAAATGCTTATTCATGGCAGGACCAACTGTGCATTTCAATC21870.20386875214985054No Hit
GCTGTGCCTTCAGGGTTCTCGGGCTGCCACGCCGATGGCGAGAGCGGCAG21240.19799598974224167No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT21000.19575874692029543No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA20580.19184357198188953No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA20410.19025885831634426No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT20160.1879283970434836No Hit
GGAGAGCCAAGCCAGGCAGACGTGCAGCCACTGGGCCCCAGCAGCCTGGC20040.1868097756325105No Hit
GGTTCTCTAGCAGCTGGGACATTTCTCTGATAATCAATGAGAACTGCATC20010.1865301202797672No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG19780.18438609590873542No Hit
GGAGAGCCGAGCCAGGCAGACGTGCAGCCACTGGGCCCCAGCAGCCTGGC19750.18410644055599212No Hit
AGGCAGTTCTTTGGTTAATATTTGTTTCAACTGATTTTCCCATGTTTCTT19670.18336069294867674No Hit
GATGTTATCTTTACTCAGACGGCTCCTCTAACCATGTTTACAACTATCAA19400.1808437947739872No Hit
GGAGGGTGTACCAGCCTGGAGGGTGTACCAGCCCCAAGTGGATGCACTTA18940.1765557460319236No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC18540.17282700799534653No Hit
GGTTATAATGTGAAACAAAACTCACCTGGGTCACTTTGATATGGTCTTGG18520.1726405710935177No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT18420.17170838658437343No Hit
AAGCAGTGAGCACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTC18200.16965758066425604No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG18170.16937792531151277No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA18120.16891183305694063No Hit
GCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCAGGTGG17870.16658137178407997No Hit
GGACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTG17870.16658137178407997No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG17820.16611527952950783No Hit
GGGTCATCCCACCTGCAGTCCCAGCCCACAGCCCCCCAGGCCCAGGAGCT17520.16331872600207506No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT17480.16294585219841734No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT17280.16108148318012883No Hit
AGAGGGGCAGCAGGAGCTGTCACCTGCAGTGCACCACGACCGGCCCTGCA17260.16089504627829995No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA17200.1603357355728134No Hit
GGGTGGGACCCCCTGGGCGCTGGGCCTCGGAGTCTGACCCGCCCTGCCCT16880.15735274514355177No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG16840.15697987133989405No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA16780.15642056063440749No Hit
GGAACATTCTCCAACACTTCCACATGCAATTCTTCTCCAGTAAGCCAGGA16650.15520872077251993No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG16620.15492906541977666Illumina Single End PCR Primer 1 (98% over 50bp)
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC16570.15446297316520455No Hit
GAGGCAGGCTAGGAATTCTGTCTGGAGATGCAGAGGCACGTGCCAAGTTG16540.15418331781246125No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA16420.15306469640148815No Hit
GGAAAATGGCTGCTGCACCCCCAAGGAGGGCCGAGGAGCCCCTGCAGAAG16390.15278504104874485No Hit
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT16370.152598604146916No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC16350.15241216724508716No Hit
AGGCAGAGCAGAATTTTAGGAGCAAAAGCTCCACATATCTTACCTCCATA16290.1518528565396006No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT16150.15054779822679862No Hit
GGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT15970.14886986611033895No Hit
AGGCCACCAACACAAATCTGAATACTGAGAGGAGAAAGATACACACACAC15810.14737837089570813No Hit
GAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGCAAGGGGCTCTTACC15720.1465394048374783No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT15680.1461665310338206No Hit
AGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAG15490.14439538046644648No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT15470.14420894356461764No Hit
CCATCATCACAGGACTGAAAAGGGAGTTCCAATTCTCACGTCAAAGGTAC15110.1408530793316983No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC15070.14048020552804058No Hit
AGGATCCCGGTCTTCAAAGGCACCAGAAAACCCCAGGGCACCTATCCTCA15060.14038698707712616No Hit
GGCACTGTCTTCCCTAAGGGGCCCTGTTCTCTGGGACCTTCCCCAGCACT15020.14001411327346844No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG14970.13954802101889632No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAG14850.1384293996079232No Hit
AGAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGC14360.1338616955131163No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG14360.1338616955131163No Hit
GGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAAGCCAAGCCAA14340.13367525861128746No Hit
GTAGTTGGGGGTTAAGCTTTGTGGATGTAGCCTTTATATTTAGTATAATT14170.1320905449457422No Hit
GAGCCGCCGCCGCCGGCCATGGAGGTGGCGCCGGAGCAGCCGCGCTGGAT14070.13115836043659795No Hit
GGAGTTTTAGAAGAACACCACCACTACCCCAACCAAAGTAACACAACACT14010.1305990497311114No Hit
GGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGA13910.12966686522196713No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT13880.12938720986922383No Hit
AGAATGACATTCTGGATGGGTGTTTCCGCGGTGAAGTTGGGAAGCTGATA13750.12817537000733628No Hit
GCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAA13680.12752284085093532No Hit
GAGGGAGGTCATACCCTCCACATCGGGAGGGGGGCGGCCGTAGCTCATAG13410.1250059426762458No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC13330.12426019506893038No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG13180.122861918305214No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG13080.12192973379606972No Hit
GGTGTTTAGGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCAC13050.12165007844332644No Hit
GGGCGGCTACTCCTGCAAGGTGGGGGTCCCTCCTAGGGTAAGGGTTGTGG12860.11987892787595235No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT12820.11950605407229464No Hit
GAGAGACCAATACATGAGGACAGGCGAAGGCTTCCTCTGTGTATTTGCCA12740.11876030646497922No Hit
GGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATATATTTTTG12570.11717559279943399No Hit
GGGACACCGATGGGCCTGCATCTGACCTGTTGTGCTCACTGCTTAGGACC12350.11512478687931658No Hit
GGCAAGATGGCACAGCCCATAAGAATAGCTATCTGTTGTACAATCTTAAT12290.11456547617383003No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG12280.11447225772291561No Hit
GGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCA12270.11437903927200119No Hit
AGGTGACATGTATTTTGCTCTGTTCAATTTGAGTTATGTCTGTTTTTAAG12140.11316719941011363No Hit
GGAAGACACCTTGTGCGTCGGCAGACACAGCCGCATGCAGCGGGGTGCGG12140.11316719941011363No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA12120.11298076250828479No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA12080.11260788870462708No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG11910.11102317503908184No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA11880.11074351968633855No Hit
GCTGCTCTTCCTCCTCTCCCCTACGTCTCTTGCTCCTAGATCTTCACGCT11740.10943846137353658No Hit
GCACAAGTTAGGTTTGTTTTGTTTTGCCTCACAGTTTGCTGTTTGTCTCC11700.10906558756987887No Hit
GGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGGAGCTGCACTG11600.1081334030607346No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG11590.10804018460982018No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC11570.10785374770799135No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT11560.10776052925707692No Hit
GCAGCAATTGATGTCACGTATCCAGCAAATCTGCTGTCACTTCCACCATA11350.10580294178787394No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT11230.10468432037690084No Hit
GCGGAAGCACCAGCTGAGAAGGTGGAGGGGACAGGATGGTACCTACCGTA11230.10468432037690084No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG11220.10459110192598642No Hit
AGGTACAGCTCCAGAGAGTAGAACAGGGCAGGCACAGGGCCCTTACCCTG10900.10160811149672477No Hit
CGAGACAAGGAGCAAACACGAGATCTTGTGCCCCCAACACAGCACTATCT10900.10160811149672477No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT10860.10123523769306707No Hit
GAACGTGGCGGCCAAGCCCGAGATGGCGGCGCTGGGTGGGGGCGGCCGGC10740.10011661628209394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)