FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002435180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185536
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACACACACACACACACACACACACACACACACACACACACACACACAC17553514.806382935651047No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC588764.966192507017922TruSeq Adapter, Index 7 (100% over 50bp)
AGTCCTGGCTCGCACGGCTCGGGCAAGCCTCGAGATCCGGCGACCCCAAA91770.7740802472468149No Hit
GATTGTCCGCCCAGCTCCATCGTCTACCTGGAGATTGACAACCGGCAGTG88320.7449794860721226No Hit
GAATGGTCAATGCGAGTGGCTGTCCCGGCTGCAGAGCGGCATGGTGCCGA64450.5436359587562082No Hit
AGTCAGATGCACAGGCCAATTAAGGTGGAACCTGGATGCAAGCCACATGC59910.5053410440509609No Hit
GACCTCCCTCAAGGTGGACAACCTGACCTACCGCACCTCGCCCGACACGC57180.48231348520837836No Hit
GAAAGCGAACGAAGGCGAAGCCGCGGGACTCCTTGGTGTAGCGATCCCGC56250.47446893219606995No Hit
GCCTCTTCCGTGGAACACACACATGGTGAACTCCTGGAAAAAACACTGTC54640.4608885769812136No Hit
AGCTGTGCTGCGAGGGGGGCGTCAGGAACTGGGCTGCGGTCACGGGTGGG49670.41896661088317855No Hit
GATCTCACAGGACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGT49550.4179544104944936No Hit
GTTATGTTCAGGCTGACGACTGCAAGAGAAAACTGACCACTACATTGGAG49220.41517085942561005No Hit
GAACTGGTGTCCCGGATATCAGCGATCTTCTCTACTTTCATTGGGGAGCA48080.40555495573310296No Hit
GACTATGGGCTTGTTTTCTCCAGATTTACTCCAACTTAGCAAACTGCAGC47630.40175920427553447No Hit
AGCACTATGATGTCTCCCAGAAGGAGGCTGGTCGTGTGTCCACCTCATCA46590.3929868009069315No Hit
GTTCTGATAATATATTCCCGTTTTTAGGGAGCAGATTAAGCGAGTAAAAG45980.3878414489311164No Hit
GTCTTTCTCGGTGCCGAAGATGGAGGCCAGATACTCCGCCATTTCCCACC45640.3849735478298424No Hit
GAAACAGCCCAAACCAGAATCCAGAGAACACATACCATCTGTTGAGCCCC42940.36219903908443096No Hit
AAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCAGAAGGCCGGGAGTGT42440.3579815374649104No Hit
GTACATCTGACCTGAATAGTCTGTGATTGATCTTGTTTCTTTAGCCCTGG42200.3559571366875405No Hit
GTCCCCAGAACCTTGTGCACTGGTGTGACTCCTCGCCCTGCAAGAACGGC40290.3398462805009717No Hit
AATAGTTCAACCAGATCAGAATTTCACAGGATTGATTGCTGGTGTTGTCT39600.33402612826603323No Hit
GACCTGCCAGGTTTGTGAATGAATTTCTGGAGCCGAGCTTTCACTTCGTC38950.3285433761606565No Hit
GTAATGACTGTGTTCTTAAGGTAACTTCTAAAAATGAACAGTAACAAAAA38560.3252537248974304No Hit
GATCCGTGATGTCCCGGTTGGCAAAGTGGTCCAGCAGCACCTTGGCGGTC36620.3088898186136903No Hit
GATTCAGCGAATCTTCACAACCAAATCCATAAACCCCAAGTGACATGTGC32480.2739689052040596No Hit
AAGACTGTATCTTTAAGGATTTTTCCCCCTTTCTTATAAGACCGCATCTT32310.2725349546534226No Hit
AACAGGTTAGATAGTTTCAAAATTAAGACTCACCATGGAGGTATCCAGAC32010.2700044536817102No Hit
GTTCAGCCTAGAAGATATCCAACCCCCACAAGAAAAATAAACTGGTTAAT30290.25549624811055927No Hit
GAAACTCTGCTTGCTATTCAAAGCAGTCAGCTCACCATTTAGAGTTAATG29810.25144744655581946No Hit
GTTTTTCAGTCTTTATTCAATTGAAGTGAAGAATATTTTTCTTTGTATGT28490.24031324228028506No Hit
CATTCTGTTTTGTTCTCTATCGTGTCCCCACAGGGAAAAGCTTCACTCTG27850.23491484020729866No Hit
GAATAAGGTAACTGTCCAGTCATCAATTCATACAGAACAATTCCAAATGC27490.23187823904124377No Hit
GCCCCTGACTCGGGGTGGGGCCAGAGGGAAGAGAGGGGAGACACACCTTG27460.23162518894407255No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC27070.22833553768084647TruSeq Adapter, Index 7 (98% over 50bp)
GTTTAAAATAGTCACCATGTACCTAAATTAAAACATAACTTTTTTTTCCC27070.22833553768084647No Hit
ATTCAGGCTGGTTAGATTAGTGATTTTAATATGAAACCATTGCTTTTAGA25920.21863528395594906No Hit
ATCACTGTCTGTATCCTTTGATTCCAGCACATTAATGGTGGGGGTGCTGG25550.21551433275750378No Hit
GTTAACTACAGTCTTCAGAAATTTCCAGTGAGCTCTCAAAAAACGTGGGT25530.21534563269272294No Hit
GCCACGCCACTCACCTGTGGCTGGCTTCCGGAAGCCTCTCAGGAGCGGGG25430.21450213236881882No Hit
GTCTATATTAGCAAGCACTGGCAAATTGGCTTAAATAAAACCCAGGGTTG25230.21281513172101058No Hit
ACTCCAGGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCT25170.2123090315266681No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTAA25030.21112813107320236No Hit
GCAATTGCATCAAAGCAACAAATACTTACACTCTGAACTACATTGACAAA24250.20454882854675013No Hit
GTTTTCCTTTACTTACTACACCTCAGATATATTTCTTCATGAAGACCTCA23980.202271377672209No Hit
GTTGTTTTAAAAGGACAAAAAAGACAGTAATGAGTATCTAATGACTTACA23810.20083742712157204No Hit
AACTGCCCTGGTCTTTGTCCCAGGAGGAAGAAGACCTGGCGGCAGGTGTG23420.19754777585834593No Hit
GAACAGAACTTCATTCAAAATCTCAGTTTGTTTCTCTGCACCTTTCTTAA22910.19324592420643488No Hit
GATTTCATTGTTTTAAGGAAATTATTTGATTCTGTATGCCATGACCCTTA22890.19307722414165407No Hit
GACCTCAGACAGGCAGGTGGGGTCGTGGTAGCCCTCGGGGCAGCGGCAGG22840.192655473979702No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA22780.19214937378535954No Hit
GCAATAATGTCTCAGGGGTGGGCTGACGGGTTTCCTCTTCCTCCTCTCAG22590.1905467231699417No Hit
AACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCC22540.19012497300798964No Hit
AATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAG22410.18902842258691427No Hit
GAACAGAAAAGAGGCAAAGATAATAAAGCTATTATTGCATCAAATATCAT22400.18894407255452386No Hit
AACCACCCAGGGTGGTTTGATACGTGAAAGTTGAATCTGCAAAAAAAGAA21640.1825334700928525No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAG21130.17823161844094149No Hit
AGTCCCAACTACTAAGGAGGCTGAGCAGGAGGATCACTTGAGCCCAAAGG20690.17452021701576334No Hit
GAACCTCTTGAGCTGTCTCAGTCGCATGTACTCTGATTTTACACGCTTCC20640.17409846685381128No Hit
CATCAGGGGCTGTCCTTCCCTACTGCTTTCCTTTCCTCATCGCAGGTGTC20620.17392976678903044No Hit
GCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACGA20440.172411466206003No Hit
ACAAGGATGGGGAGGCTAAGACTGACCCAGCAGGGCTGAGCAGTCCCCAT20060.16920616497516736No Hit
ACATGCTTCTGTGTGACGCTGCGGACGTCCCCGACGTACATGATCTTCCC20050.16912181494277695No Hit
AAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGGT19840.16735046426257827No Hit
GATTTCTCTAATAGCTGCCACATTAGGACCTGCTCCTAGATGGGTATAAA19760.166675664003455No Hit
GCTTACTTTTTTCTTTTTAGATTTTGTGGTGGATGCAACCCGCAAGGGTA19240.16228946231915353No Hit
AGAAGTACCTCTCTCTGTTTTGGGGGTTTCATCCCATCTGTTTTTACGAG19080.1609398618009069No Hit
GTATCCAAGAAAATCAGTGCATGGGCATGGGACTCAGAGTAGAGATAGGT19050.1606868117037357No Hit
ACTATTCTAAAACAAATGAAAACAAAGCAGATATTGTTAAGCTAATAATG19000.16026506154178363No Hit
AATAACCACGACCAGGAATTTGTGAGTGCTGGGCCTGGGGCGCGGTCTCG18930.15967461131505073No Hit
CACCTCGTACTCTGGCTCGTCATCGCCTGCTTTGGTGGCATTCTTGTCCC18890.1593372111854891No Hit
CCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCAT18770.15832501079680414No Hit
GCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGAC18180.1533483588857698No Hit
GTCCCCACGCGGAAGGCAACTGCCTGAGAGGCGCGGCGTCGCACCGCCCA18140.15301095875620815No Hit
CATGAGATGGATGGCCACTTCATGGGAGCCACCTCTAGATTACCACCCAA18110.15275790865903693No Hit
GCTATTTCTTTTTCTTTTCTCTGCTTAGGAAATTGAACTTAGCTCATTAA18020.15199875836752322No Hit
GCTTGATAGGTAGGTACTCACCTATTCTCACAGATCTCCTTTTGTCGGCC17950.15140830814079032No Hit
CCTGGGACAAGGCGGCCAGCACCTCTTGGGCCTGCACCCCTCACCTGTAG17940.1513239581083999No Hit
GTATATCAGTTTAGTCCAGAGAATGTTATTTGCTAATTTAAGGTGATAAT17880.15081785791405744No Hit
CCTTGTCTCGACCCTTCAGAATCTCTTGCTCTTTGTTTCTCATCAACTGC17860.1506491578492766No Hit
AGAAACAAGGTTGAATAAGGTTTAGAATGCAGAGATGAGAGCTTTACATG17690.1492152072986396No Hit
CCAGTCGACTCTCAAGTAAGCCACTTGAAAACACATTCTTTGCAGCTGAG17680.1491308572662492No Hit
AGCCTTGGCAAGCAGACCTTTAGCCACGACCCAGACCATCCTTCCTGGGA17600.14845605700712589No Hit
ATACTATCCCACACTTGATTAGGGAGTAACATGTACTTTTCTATCAAGTG17580.14828735694234507No Hit
GCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCTTTACAGAGGT17430.1470221064564889No Hit
GACTCTAAGGGAAAATTTCTGAAATTATGTGTCTTCAGGATTGTATCTCT17210.1451664057438998No Hit
ATAACATAAACGTTAAAATGTCTGCTTTTTAAAATAGGTTCATACGTTTT17140.14457595551716693No Hit
GCACCTTTCAAGGATCACTTTTACTTTTTGCCCCAGCTAAATCATCTAAG17130.14449160548477652No Hit
GATTGAAGAAGACGTCCACCTCGTCTGGAGGCAGCAGCTGCGCGGGTTCC17060.14390115525804362No Hit
GACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGACCGCTCTGCATCT17030.1436481051608724No Hit
GAACAGGAATGACATACAGACTGCAGGGACAATGACTGTTCCATTGTGTT16870.14229850464262578No Hit
ATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC16790.1416237043835025No Hit
GACCATGAATGATGGCCAGTTCTGCCTGTGGGTCTTCCTCAGTCCCATCC16710.1409489041243792No Hit
AAAACATAATTCCACAACAAAGATAGAAAATGAAAACGTACAATAATTGC16540.13951495357374216No Hit
CATCAACTCTTGATAGTGACCAAATTTTTCCTTGTTGTCCTTCTGATGGG16320.1376592528611531No Hit
ACTTCTTTGACTTTAGTGGCACTTGGACTGCAATGCTTTACCTTGGACCT16250.1370688026344202No Hit
ACCAGGGCGCCAGCCTGCACAACCAGACAGACCGCACGGGCGAGACCGCC15930.13436960159792702No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG15860.13377915137119414No Hit
GACCCATGAGTGCCCTTCTACATGTCCCACTTGATTCAGTCATGACTTGT15740.13276695098250918No Hit
GACAGATAACGTACCTCTTCCACTTCGACCGACAAACCTGAGGTCATTAA15710.13251390088533793No Hit
AGTACATGCTCCATGAACAACCAAAAGAGAAGGAGGCACCACAGGTTTCT15510.1308269002375297No Hit
GTCCTCTTCACCCTCACATTGCTCCCCAGGTGGCAGTTTTGGCACAGGAA15490.13065820017274887No Hit
GTATTGTTTGGAGGTCATTTGATTGGAGAGATTGGGTTGATACTGAAGAA15320.12922424962211185No Hit
AGCACAACAAAGACACTGAAACATTATTTTGTTTCCACCACTTATTTTTA15300.12905554955733103No Hit
CATTCCTGTTCACACAATTATTCTGAGGCCTTTCAGAAAGCATCGGAGAA15030.12677809868278989No Hit
ATCTTCAGCATGAGGTATATGAATATTTTTAGGTGTAATAACCAAGAAAG15020.12669374865039948No Hit
GCATTTAAAATAAAAATAATTTTATGTGTTTAATTATTGCATTCTGATCA14660.12365714748434464No Hit
ATTTGTCTCAATTAAGAGTGGGCTCTTTAACACCATTGTTTTAAAAAAAT14640.12348844741956382No Hit
GCACCTGGCATCCTCGTGCTCCTGTTTACCTTGGTGCAGAGGAGCAATGG14540.12264494709565968No Hit
AAAGCTTCTGTTTATGTCTGTTCATAGTTATATCTTCCGGCTGAGCTGTA14490.12222319693370762No Hit
ATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTTGACGG14170.11952399589721442No Hit
GAAAGCCCAAATGATGGAATCCAAAACAAGTTTAAACTGTGTAGGTGGAA14140.11927094580004317No Hit
GGACACGCTGCGGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCA14120.11910224573526236No Hit
GTTTAATATACTTCAGGCTGCGTATGTCAGCAAGCCTGGGGCCCAGCTTG14120.11910224573526236No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC14080.11876484560570072TruSeq Adapter, Index 7 (98% over 50bp)
AAGCAGCTCTCTGTTGGATTTGTAGCTTCCCGCAGAAATTTGGTTTAATT14050.11851179550852947No Hit
AACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGGTTC13970.11783699524940618No Hit
AAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTTGAGAA13800.11640304469876916No Hit
ATCCTCCGTGGTCATGCTCCAATAAATTCACTGCTTTGTGGCGCGACCCT13650.11513779421291298No Hit
CCTCCCACATGGCCCCAGGGTCTGCCTACCACCCCAGGCCACGCACTTGC13600.11471604405096092No Hit
AGTTCAGTGCAAAACTTAATTTTAATATTAAAATTTTTCTCATGCCCTAT13590.1146316940185705No Hit
AATTAAACTCAATAAAAAACACCTAGAAACTGTGGGGAGGGTCCACTTTG13520.11404124379183762No Hit
GAACAGTGCATGGGCCTATCAGGTTCAGTTTTCTCCACTGGGCCAGCTCC13440.11336644353271431No Hit
ACACAGAATCCTAATAATCAGGCATACCATCTCGGTGATCCTCCAGATCT13420.1131977434679335No Hit
CAAGTAGCATGTCGCCCAAGAGCAGAAGTCAAACCATACCCAATAGAGTC13410.11311339343554308No Hit
GACATTTGATGCGTTTCAGAATGTGGGTTTATAAATGATGAAATTTTTGT13370.11277599330598143No Hit
GCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCTTGCGCA13260.1118481429496869No Hit
GCACAAGTTAGGTTTGTTTTGTTTTGCCTCACAGTTTGCTGTTTGTCTCC13190.111257692722954No Hit
ATACAAAGAGCTAAATAGGCGAATTGATCTAAAACAGATACATTCAAATT13030.1099080922047074No Hit
GCCTCTTTTCTGTTCACATAATCCTAATAGATCCTGTAAATAAGACAAAT12870.1085584916864608No Hit
ACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCAGACATCAC12830.10822109155689916No Hit
AAAATTGTTGCTTTCAAAGGCATGGACATACTTAATGGGGTAAGAATCTC12780.1077993413949471No Hit
CAGCAGTTCAGCTCTCACCTGTCTCTGGGGCAGCACCGTGCCCTGCTGTG12560.10594364068235802No Hit
GACCATTCAAACTGGTGGACCCGCCCACAGTGAAATTCAGGGCCCCTCCG12540.1057749406175772No Hit
GCAAGTGACCCTTGTTTTGTTTTGGTTGGGGTGGGGGGTGTTTGGGATGG12520.10560624055279638No Hit
GCACAGCCTATATAATGCTATCCATAGCAATGAATTTGGTCTTTTGATTT12510.10552189052040596No Hit
ACAGGCCTTCGGCTCCACCTGGTCCACCGCCAGTCTCCTGCCTGGCGGGG12390.104509690131721No Hit
GTCCCTGGACAGCAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGG12330.10400358993737854No Hit
GTCTGAGGCCAGCAAGAAGAAGCGGCGGGAGCCCCTCGGCGAGGACTCCG12300.10375053984020731No Hit
CTTCTGCCGTCAGAGTTTCAGCTGGGTTGGGGTGGATGCAGCCACCTCCA12200.10290703951630319No Hit
AATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGGAAACTAATAGT12170.10265398941913194No Hit
ACTTACATACAGTTTCTTGCAGCCAAGTCTCTGTGGACAAACTTTTTGCT12100.10206353919239905No Hit
GACAAAGGGATCTTCGGTGGACAAGGATGAGAAACCCAATTGGAACCAAT12030.10147308896566616No Hit
CATGCTCCACTACTTACAGGGTATGGCTCTCTCGGCATGATGACCAGCGT11860.10003913841502915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)