FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002435203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16206630
Sequences flagged as poor quality0
Sequence length251
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC2487471.5348471582309215No Hit
TGGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1764961.0890357835034181No Hit
GGGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA1630511.006075908439941No Hit
GTGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1547470.9548376189250942No Hit
CGGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1544890.9532456778491272No Hit
GGTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1445780.89209169333785No Hit
TTGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1377980.8502569627368552No Hit
AGGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1328720.8198619947515307No Hit
TGTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1123620.6933088495263975No Hit
GTTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1087620.6710957182338338No Hit
GAGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1084560.669207602073966No Hit
GCGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1051610.6488764166270224No Hit
CTGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC991430.6117434654829536No Hit
TAGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC976640.6026175707102588No Hit
GGCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC952970.5880124368853982No Hit
ATGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC931300.5746413659101245No Hit
TCGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC893810.5515088577946187No Hit
TTTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC858730.529863395412865No Hit
CAGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC847000.522625616800038No Hit
CGGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA819550.5056881041894582No Hit
GGATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC794560.4902684888838704No Hit
CGTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC780640.4816794114507457No Hit
AGTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC764940.4719920180814889No Hit
AAGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC735880.4540610848769917No Hit
GGTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA715900.44173279700961887No Hit
CCGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC708550.43719761603738716No Hit
GTCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC701460.4328228632356017No Hit
TGCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC700710.4323600896670067No Hit
GCTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC688210.42464719685708874No Hit
GATTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC665690.4107516491707406No Hit
ACGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC658990.4066175386246246No Hit
TGGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA658010.40601284782832703No Hit
GGGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA636640.3928268862804914No Hit
TGATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC633040.390605573151235No Hit
GGCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA630840.38924810401668947No Hit
GTATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC613160.37833898842634156No Hit
GGAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA596250.3679049870330846No Hit
GTGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA579130.357341409040621No Hit
CGCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC571660.3527321842974141No Hit
GCGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA571390.35256558581271985No Hit
TATTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC555060.3424894626458431No Hit
ATTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC542410.33468401512220614No Hit
AGCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC523630.3230961649645855No Hit
CGATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC523330.32291105553714744No Hit
GAGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA510910.31524752524121297No Hit
CGTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA504110.3110517115526176No Hit
TCTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC492900.30413478928068327No Hit
CATTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC490050.302376249720022No Hit
CTTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC490000.3023453981487823No Hit
TTATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC483400.2982729907451457No Hit
AGGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA478500.29524953676365784No Hit
GCCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC472260.2913992606729468No Hit
GAATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC466880.2880796316075581No Hit
TTCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC463100.285747252821839No Hit
GCATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC459650.2836184944063016No Hit
GACTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC457120.2820574049015742No Hit
AGATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC457090.2820388939588304No Hit
AATTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC454350.28034822785489644No Hit
TGTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA447660.27622028762302836No Hit
TAATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC423570.26135600059975456No Hit
GTTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA422440.26065875508973796No Hit
CGAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA415350.25628400228795256No Hit
CCTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC412120.25429099078586975No Hit
CGCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA410610.25335927333443164No Hit
ATATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC402960.24863898293476186No Hit
CTATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC402660.24845387350732384No Hit
TCATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC400510.24712725594401797No Hit
CAGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA391760.24172823097707544No Hit
TAGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA383280.2364958044948271No Hit
ACTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC382620.23608856375446344No Hit
GTAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA380370.23470024304867826No Hit
TGAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA378480.23353405365581864No Hit
CAATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC377210.23275042374633095No Hit
TACTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC376330.23220743609251274No Hit
CCGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA375500.2316953000099342No Hit
CTGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA374670.23118316392735566No Hit
CACTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC371530.22924568525350425No Hit
ATCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC370540.22863482414295877No Hit
TTGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA366440.2261049953013057No Hit
TCGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA364220.22473518553826427No Hit
CTCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC363590.2243464557406444No Hit
TTTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA360870.22266813026520627No Hit
CCATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC358820.22140321584437975No Hit
GCAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA354270.21859572286156964No Hit
CATAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA342990.2116356083898997No Hit
TGCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA342810.21152454273343688No Hit
AGTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA341420.210666869052974No Hit
GAAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA335400.20695233987571754No Hit
GATAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA335250.20685978516199852No Hit
GCTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA333010.20547763477046124No Hit
CAAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA332440.20512592685832898No Hit
CCCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC329730.20345377169713874No Hit
TAAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA326870.20168906182222954No Hit
TTAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA324330.20012180200325425No Hit
TCCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC323180.19941221586474178No Hit
TATAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA322920.1992517876942955No Hit
GTCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA319360.19705515582203087No Hit
AAATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC315900.19492022709224557No Hit
AACTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC314830.1942600034677166No Hit
CGGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA309640.1910576103730387No Hit
ACATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC307390.18966928966725347No Hit
AAGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA290170.17904400853231056No Hit
AGAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA288300.17789015976794684No Hit
ACCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC287630.17747674871333521No Hit
GACAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA287040.17711270017270708No Hit
CTTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA286190.17658822346163267No Hit
TCAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA285270.17602055455082272No Hit
CTAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA280030.17278730988490512No Hit
ATGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA279810.17265156297145057No Hit
GCCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA278220.17167048300602902No Hit
CCAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA278210.1716643126917811No Hit
GGTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA274580.1694244886197809No Hit
TTCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA268780.165845706355979No Hit
ACGAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA267620.1651299499032186No Hit
ATTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA265920.1640809964810698No Hit
TCTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA265520.16383418391115243No Hit
CACAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA259610.16018752819062324No Hit
AGCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA259580.1601690172478794No Hit
TGGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA258060.1592311294821934No Hit
AAAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA257910.15913857476847437No Hit
TACAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA255530.157670039977466No Hit
GGCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA251080.15492425013713523No Hit
AATAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA248450.1533014574899285No Hit
GGAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA242760.14979054868285388No Hit
CCTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA238790.14734093392642394No Hit
CTCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA238180.14696454475729995No Hit
ATAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA235100.14506408796893616No Hit
GCGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA224190.1383322751244398No Hit
GTGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA221800.1368575700191835No Hit
TCCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA215810.13316155178467085No Hit
ACAAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA215630.13305048612820802No Hit
CCCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA202830.12515248389085207No Hit
AACAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA200750.12386905852728174No Hit
ACTAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA198340.12238201279352956No Hit
CGTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA197960.12214754085210805No Hit
ATCAAACAGCCCGCCTGGAATGTGTGGTGTCTGGAATAACAATTTCTGCA195580.12067900606109969No Hit
GAGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA195070.12036432003445503No Hit
AGGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA189750.11708171285455396No Hit
TGTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA179730.11089905797812376No Hit
GTTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA172270.10629600354916476No Hit
CGAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA167290.10322318705369346No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)