FastQCFastQC Report
Mon 4 Sep 2023
EGAF00002435208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19333168
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT921260.47651786815280356No Hit
TGTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT742190.3838946622715946No Hit
AATCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT650620.33653046412258975No Hit
GGGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT562720.2910645580693242No Hit
TTTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT550740.2848679533535321No Hit
TGGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA546270.28255586461566984No Hit
GGGCGTGATTGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA544970.2818834450722199No Hit
ATTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT522080.27004368864947537No Hit
CGGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT508420.26297811098522494No Hit
GGTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT508380.2629574211531188No Hit
GATCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT498920.25806427586001424No Hit
CGGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA481580.24909523364199804No Hit
TCGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA480540.24855729800723814No Hit
GGGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA480520.24854695309118507No Hit
ATACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT478850.24768315260075327No Hit
TGGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT478520.24751246148587755No Hit
AGGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA478080.24728487333270988No Hit
GAGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT473860.245102096045511No Hit
CATCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT472370.2443313997995569No Hit
TTCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT468170.24215896742841111No Hit
TGTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA466060.24106757878481166No Hit
AGTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT461010.23845548748141018No Hit
TTACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT446580.2309916305491164No Hit
GTTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT445270.23031403854764No Hit
GTCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA444810.23007610547841925No Hit
GCTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT439570.22736573747251357No Hit
AGTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA434330.22465536946660786No Hit
GGGCGTGATTGGGGGAAACATCTGCATCACATTCCAGGCGGGCTGTTTCC426320.22051223058735123No Hit
ACTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT425810.2202484352279978No Hit
TAACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT422650.2186139384916119No Hit
AGGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT410650.21240698885976678No Hit
CGTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT405160.20956730940319765No Hit
TTGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA403710.2088173029893497No Hit
TAGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT402760.20832591947682863No Hit
ATCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT399440.2066086634120181No Hit
GTACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT394930.2042758848420497No Hit
AAGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT391060.20227414358577964No Hit
TGGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT378550.19580339859458107No Hit
GGGCGTGATTGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT372830.1928447526034016No Hit
ATGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT363770.1881585056313585No Hit
GTCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT361490.18697918520130793No Hit
GTGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT356500.18439812864606567No Hit
GGGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT351640.1818843140451684No Hit
GCGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT348330.18017223043838446No Hit
GGCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT348040.18002222915561486No Hit
GGGCGTGATTGGGGGAAACATCTGCATCAAGTTGTTTATCTGTAATGATA347650.17982050329257987No Hit
GGACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT346370.17915842866518306No Hit
AGGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT345960.17894635788609503No Hit
GGAATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA340730.17624116233821585No Hit
TCGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT339440.1755739152527925No Hit
GCGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA333780.1726463040097722No Hit
CGGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT333300.1723980260244984No Hit
TTTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA331630.17153422553406664No Hit
TGTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT331520.1714773284957747No Hit
CTTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT330440.17091870302890866No Hit
GGCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA326670.168968686352904No Hit
GGTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA324680.167939367205623No Hit
CAGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT323300.16722556799796082No Hit
TTGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT322750.16694108280650125No Hit
CGTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA314120.1624772515295993No Hit
AGTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT310020.16035654373871888No Hit
TGACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT308980.15981860810395898No Hit
GTCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT307800.1592082580568275No Hit
ACGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT307370.1589858423616864No Hit
GAGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA305390.15796169567243196No Hit
GCACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT304120.1573047935030617No Hit
GTTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA303260.15685996211277944No Hit
AGCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT297180.1537151076326446No Hit
GAACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT295650.15292372155458434No Hit
CAACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT292900.1515012955972865No Hit
TCTCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT292690.15139267397872921No Hit
CTCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT285130.1474822957106668No Hit
TACCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT284550.1471822931451276No Hit
AGACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT283970.1468822905795884No Hit
GTGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA283960.14687711812156187No Hit
TGCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT283840.14681504862524342No Hit
TCACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT280300.1449839984838491No Hit
CTACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT279830.14474089295660184No Hit
TAGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA279540.14459089167383224No Hit
TCGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT279420.14452882217751378No Hit
AACCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT275730.14262018516572142No Hit
ACACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT274440.14195293808029807No Hit
TTGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT270810.14007533581666493No Hit
AGCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA269910.13960981459427654No Hit
CGACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT266080.13762876317011263No Hit
AAACGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT265580.13737014026878575No Hit
GGGGTCCAGAAAGGAGGGATTCCAGATCGGAAGAGCGGTTCAGCAGGAAT262520.13578736811266523Illumina Paired End PCR Primer 2 (100% over 27bp)
GGGCGTGATTGGGGGAAACATCTGCATCACATTCCAGGCGGGCTGTTTAC262210.13562702191384257No Hit
ATGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA260020.13449425360603084No Hit
TGCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA258030.13346493445874985No Hit
GCGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT252210.13045456388730495No Hit
GTAATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA251500.1300873193674208No Hit
AAGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA251180.1299218007105716No Hit
TTCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA246360.12742867594178045No Hit
TTTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT244560.12649763349700371No Hit
TTAATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA241550.12494072363101588No Hit
GCCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT240670.12448554732468056No Hit
ATTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA240030.12415451001098216No Hit
TATATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA237650.1229234650006662No Hit
CGCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT237320.12275277388579048No Hit
ACCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT234050.12106138011111268No Hit
TCTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA233740.12090103391229001No Hit
GGAATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT233430.12074068771346733No Hit
GACCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT232060.1200320609638317No Hit
TGGCGTGATTGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA231650.11981999018474364No Hit
GGCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT230500.11922515751169181No Hit
GCTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA229280.11859411763245424No Hit
GGGCGTGATTGGGGGAAACATCTGCATCACATTCCAGGCGGGCTGTTTCA227230.11753376373701402No Hit
GGGCGTGATTGGGGGAAACATCTGCATCACATTCCAGGCGGGCTGTTTTC226620.11721824379739523No Hit
CGTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT225930.11686134419356413No Hit
ACGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA225330.11655099671197189No Hit
CACCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT224850.11630271872669809No Hit
GGGCGTGATTGGGGGAAACATCTGCATCACATTCCAGGCGGGCTGTTTCT222690.11518546779296596No Hit
GGGCGTGATTGGGGGAAACATCTGCATCACATTCCAGGCGGGCTGTTTGC220410.11400614736291538No Hit
CTGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA220320.11395959524067655No Hit
GAGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT218260.11289406888720979No Hit
CTGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT217160.11232509850429065No Hit
GGGCGTGATTGGGGGAAACATCTGCATCACATTCCAGGCGGGCTGTTTCG216430.1119475090683534No Hit
CTTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA215380.11140440097556696No Hit
GTGCGTGATTGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA215280.1113526763953016No Hit
GGTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT215220.11132164164714235No Hit
GTTATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT213280.11031818478999407No Hit
AGAATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA212460.10989404323181798No Hit
TCCCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT210880.10907679486362504No Hit
GTGATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT209800.10851816939675897No Hit
CCGCGCTCTACGGATGGTTTGGTATGAGGCTGACTTCTTGGTTCCACAGT209560.10839403040412209No Hit
AGGCGTGATTGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA203160.105083657267138No Hit
CGCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA202990.10499572548068688No Hit
GGTCGTGATTGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA200690.10380606013458323No Hit
GATATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCTGTGACAACA196230.10149914385474744No Hit
AGCATGATATGGGGGAAACATCTGCATCAAGTTGTTTATCAGGTGAAGTT196000.1013801773201371No Hit
GGGCGTGATTGGGGGAAACATCTGCATCACATTCCAGGCGGGCTGTTTAA193440.10005602806534344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)