FastQCFastQC Report
Mon 3 Apr 2023
EGAF00002435209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002435209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19333168
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA2422001.2527693340274082No Hit
GGGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1478000.7644892963222581No Hit
CGGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA1182060.6114155734849043No Hit
GGTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA1059420.5479805482474471No Hit
CGGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC1024190.5297579786199551No Hit
TGGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA1014220.5246010379674971No Hit
TGGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC990470.5123164501544702No Hit
GGCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA953670.4932818046168119No Hit
GGAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA897430.46419190067556443No Hit
GCGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA878890.45460216349436366No Hit
GTGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC861310.44550898228371055No Hit
GTGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA857230.4433986194088832No Hit
TTGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC815410.42176739994190293No Hit
GGTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC810990.4194811734941733No Hit
GAGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA771930.3992775524425175No Hit
AGGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA744190.38492915387690213No Hit
CGTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA716210.3704566163186499No Hit
AGGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC697340.3606961880225734No Hit
TGTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA697320.36068584310652035No Hit
GTTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC672310.34774952558214983No Hit
GTTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA660720.3417546467293927No Hit
TGTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC659540.34114429668226126No Hit
GAGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC642980.332578706190315No Hit
GCGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC635930.328932123281606No Hit
CTGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC634900.32839936010487264No Hit
CGAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA611300.31619235916224386No Hit
CGCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA610090.3155664917410328No Hit
CAGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA603330.3120699101150934No Hit
TAGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC601870.3113147312432189No Hit
TTGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA598200.3094164391474796No Hit
TAGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA593870.30717676482198886No Hit
TGAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA584360.3022577572387516No Hit
GTAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA580550.3002870507306407No Hit
CCGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA579390.2996870455995624No Hit
TTTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA574140.2969715051356301No Hit
TCGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA552960.2860162390354235No Hit
TTTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC548440.28367828800742845No Hit
CTGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA546700.2827782803108109No Hit
TCGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC544540.2816610293770788No Hit
TGCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA538080.2783196214919355No Hit
ATGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC529870.2740730334521482No Hit
CGTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC529720.27399544658175007No Hit
GGCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC527650.27292474777025677No Hit
TTAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA523640.2708505921016152No Hit
CATAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA520240.2690919563725924No Hit
GCTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA518820.2683574673328241No Hit
GATAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA514420.26608158580114755No Hit
TATAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA514330.26603503367890874No Hit
GCAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA513880.2658022730677145No Hit
GAAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA513420.26556433999849377No Hit
CAGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC508640.2630919050618088No Hit
AGTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA507920.2627194880838981No Hit
CAAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA504010.2606970569955219No Hit
TAAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA501750.2595280814815244No Hit
AGTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC491040.2539883789351026No Hit
GTCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA489330.2531038886125647No Hit
CCGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC479450.24799350008234555No Hit
GGATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC462320.2391330794828866No Hit
GCTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC453170.23440028038860472No Hit
CTTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA451850.23371751592910173No Hit
TCAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA449580.2325433679570777No Hit
GCCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA442710.22898988929284636No Hit
AAGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA436750.22590710430902997No Hit
ATGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA432900.22391570796881297No Hit
GACAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA432020.22346053166247765No Hit
TGCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC430370.22260707608809893No Hit
AGAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA430360.22260190363007243No Hit
GTCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC426970.22084844035907616No Hit
ATTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA426680.2206984390763066No Hit
TCTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA423400.21900187284360226No Hit
AAGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC416810.21559322300411396No Hit
CTAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA415910.2151277017817256No Hit
CCAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA414940.21462597335315142No Hit
TTCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA413550.2139070016874627No Hit
CGCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC411110.2126449219289875No Hit
GATTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC410070.21210698629422764No Hit
ACGTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC407360.21070525016903593No Hit
AGCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA405910.20995524375518798No Hit
ACGAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA402910.2084035063472267No Hit
TACAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA395410.20452416282732347No Hit
AATAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA379660.19637754143552677No Hit
TGATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC377160.19508442692889236No Hit
GTATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC373410.19314475516894075No Hit
GGGAAACAGCCCGCCTGGAATGTGATGCAGATGTTTCCCCCAATCACGCC372220.19252923266378277No Hit
CACAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA368940.19083266643107843No Hit
ATAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA368060.19037749012474314No Hit
CCTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA367640.19016024688762856No Hit
CTCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA367600.1901395570555224No Hit
TATTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC361760.18711884156802444No Hit
AAAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA360030.18622400632943345No Hit
CGATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC351540.18183258946490302No Hit
TCCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA350120.1810981004251347No Hit
CATTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC349300.18067395886695858No Hit
ACAAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA338020.17483942621302417No Hit
ATTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC336950.17428597320418465No Hit
CTTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC336840.17422907616589273No Hit
AGCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC334620.17308079048400138No Hit
CCCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA329070.170210076279273No Hit
GAATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC319740.1653841729405134No Hit
GCCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC314470.1626582875605281No Hit
TTATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC311710.16123068914520372No Hit
ACTAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA302630.15653409725710757No Hit
ATCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA301460.15592891966800268No Hit
AACAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA299680.15500822213927898No Hit
TCTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC299230.1547754615280848No Hit
GACTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC288330.1491374822791588No Hit
GCATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC285530.14768919403172828No Hit
AGATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC276130.14282708348678294No Hit
CCTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC275360.14242880421873952No Hit
GGAATCCCTCCTTTCTGGACCCCAGATCGGAAGAGCGTCGTGTAGGGAAA275210.14235121734834144Illumina Single End PCR Primer 1 (100% over 27bp)
TAATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC271760.140566719329186No Hit
TTCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC270450.13988912732770956No Hit
AATTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC260990.134995982034605No Hit
CACTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC246480.12749074543809893No Hit
ACTTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC246440.12747005560599275No Hit
ATATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC245640.12705625896386974No Hit
CTATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC237250.1227165666796047No Hit
CCATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC237230.12270622176355163No Hit
CAATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC236940.12255622048078203No Hit
ACCAAACAGCCCGCCTGGAATGTGTGGTATCTGGAATAACAATTTCTGCA236270.12220966579300403No Hit
TCATCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC231910.11995447409343363No Hit
TACTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC227870.11786480105071243No Hit
CTCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC216600.11203544085480455No Hit
ATCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC215380.11140440097556696No Hit
CGGAAACAGCCCGCCTGGAATGTGATGCAGATGTTTCCCCCAATCACGCC214900.11115612299029316No Hit
TCCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC208460.10782506002120294No Hit
CCCTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC204730.10589573317730441No Hit
GGGAAACAGCCCGCCTGGAATGTGATGCAGATGTTTCCCCCAATCACGAC198490.10266811936874495No Hit
GGTAAACAGCCCGCCTGGAATGTGATGCAGATGTTTCCCCCAATCACGCC195370.10105431246446521No Hit
AACTCCCTGCCACCCTCAGGTGCTCCATGAAAGGAGAAGCGATCGGTAAC194120.10040775521114802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)