FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002439664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666436
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA1358515.094853204802216TruSeq Adapter, Index 6 (97% over 36bp)
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT966203.623563438237407No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC659082.4717638075693547No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT611202.2921982751508008No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT572942.1487108634896916No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC549532.060915769214037No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG458461.719373725827284No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT415211.5571721953949018No Hit
CCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAT328771.2329941539943206TruSeq Adapter, Index 6 (97% over 36bp)
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC315901.184727478926927No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT269721.0115374979935765No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA265080.994135992763374No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG263710.9889980483311807No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT240750.9028906000369032No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA211340.7925935593428832No Hit
ACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAA202330.7588031364713047TruSeq Adapter, Index 6 (97% over 36bp)
GACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGA197200.7395639722836025TruSeq Adapter, Index 6 (97% over 36bp)
GGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGG186470.6993229914387594TruSeq Adapter, Index 6 (97% over 36bp)
CGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAG181880.6821090024287101TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTC172770.6479435471168256TruSeq Adapter, Index 6 (97% over 36bp)
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC160100.6004269369300445No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG148640.557448219270967No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG148510.5569606770985691No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT144860.5432719930273969No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG132670.49755553855408496No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT126690.47512859862378093No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCG120270.45105151595613024TruSeq Adapter, Index 6 (97% over 36bp)
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG115610.4335750042378666No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA112790.42299908942123493No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC110120.41298572326506244No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA107400.4027848408887369No Hit
CACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATC106250.39847196782521693TruSeq Adapter, Index 6 (97% over 36bp)
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT100550.37709511872776996No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA97910.36719426230368923No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA94510.3544431593332823No Hit
CGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAT92980.3487051629965992TruSeq Adapter, Index 6 (97% over 36bp)
CCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTC90690.3401169201135898TruSeq Adapter, Index 6 (97% over 36bp)
GCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA77490.2906126379931864TruSeq Adapter, Index 6 (97% over 36bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCT77140.2893000244521151TruSeq Adapter, Index 6 (97% over 36bp)
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA75480.2830744859430341No Hit
TACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGG73790.2767364377018612No Hit
CGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG72490.2718610159778821TruSeq Adapter, Index 6 (97% over 36bp)
TGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA72380.2714484802935454Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTC69010.2588098870552303TruSeq Adapter, Index 6 (97% over 36bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAA66770.25040916039237393TruSeq Adapter, Index 6 (97% over 36bp)
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC65620.2460962873288539No Hit
TTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGA64400.24152089155711967No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG64190.2407333234324769No Hit
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC63950.23983324557574232No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC62910.235932908196559No Hit
TCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGT58910.2209316105843155No Hit
ACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCT56720.21271840014161228TruSeq Adapter, Index 6 (97% over 36bp)
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT53130.19925473553462375No Hit
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG53110.1991797290465625TruSeq Adapter, Index 6 (97% over 36bp)
ACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATC51530.19325421648972635No Hit
CGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAG49250.1847034768507476No Hit
ACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGA48610.18230326923278864No Hit
GGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGT45760.17161484468406518No Hit
ATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTA44000.16501427373467806No Hit
GCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATG43930.16475175102646378No Hit
GATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC41830.156876069780036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC39970.14990046639034277No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT37660.14123721701927217Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCG36090.1353492077064666No Hit
TGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAA35130.13174889627952818No Hit
ATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT34110.1279235653884061Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGAC33830.12687347455554904No Hit
CGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATC33190.1244732669375901TruSeq Adapter, Index 6 (97% over 36bp)
CGTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAT31820.1193353225053967TruSeq Adapter, Index 6 (97% over 36bp)
TCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGC31290.11734765057177445No Hit
TACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGAT31220.11708512786356019No Hit
CGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATC30750.11532247539412159TruSeq Adapter, Index 6 (97% over 36bp)
ATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC29590.11097209908657098Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT29170.10939696283728542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)