FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002439665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666436
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC1397615.241490888961895Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC879533.2985228222241223No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC789812.9620437167815017No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT589462.2106662226282574No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT536872.0134366622712863No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT442291.65873098022979No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT422111.5830494337760217No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG396521.4870786323016942No Hit
CGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG343911.2897740654566618Illumina Single End PCR Primer 1 (96% over 33bp)
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC333251.2497956073200331No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT327811.2293938425673823No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC292011.0951322289378032No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC285201.0695925197529588No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT281271.0548537448489295No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG265600.9960861614529656No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT252680.9476319701654193No Hit
CGGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGT209030.7839303099718125Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG200100.750439913052479Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC184050.6902472063833521Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT180300.6761834898718738Illumina Single End PCR Primer 1 (96% over 33bp)
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG167750.6291169186134601No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA140820.528120682439031No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG139340.522570202322501No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT138000.5175447676223993No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG129230.48465442260755554No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG128780.48296677662617815No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA121090.45412678196664014No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC117920.44223825360893715No Hit
CGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGT112120.42048637207118417Illumina Single End PCR Primer 1 (96% over 33bp)
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT109360.4101354767187362No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA104310.39119633848327884No Hit
AAGTGCAATTTCCGTTCTAAGTGCAATTTCCGTTCTAAGTGCAATTTCCG102880.3858333745869018No Hit
CCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG94370.3539181139168538Illumina Single End PCR Primer 1 (96% over 33bp)
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA94090.35286802308399673No Hit
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAG93360.35013028626976234Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGA92750.3478425883838952Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTA78050.2927128196589005Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA76520.2869748233222174Illumina Single End PCR Primer 1 (96% over 33bp)
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG74510.279436671272065No Hit
CGGTGGTCGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG73120.2742237203518105Illumina Single End PCR Primer 1 (96% over 33bp)
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC71470.26803568508676No Hit
TTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGA70230.26338528282696455No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAG68940.258547364347016Illumina Single End PCR Primer 1 (96% over 33bp)
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC66180.24819646899456801No Hit
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGT66170.24815896575053742Illumina Single End PCR Primer 1 (96% over 33bp)
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA63570.23840812230257916No Hit
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT63280.2373205282256915No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC62640.23492032060773255No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA60590.2272321555814578No Hit
TCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGT60100.22539449662395797No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT59360.22261925656569292No Hit
CGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTA59090.22160666897686648Illumina Single End PCR Primer 1 (96% over 33bp)
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT55400.20776797192957192No Hit
CGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG52690.19760459279727693Illumina Single End PCR Primer 1 (96% over 33bp)
TACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGG39540.1482878268970266No Hit
TGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAA35990.1349741752661605No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA35940.13478665904600748No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG35760.13411160065345654No Hit
TCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGC35540.13328652928478313No Hit
TACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGAT35300.13238645142804853No Hit
CACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGT33380.12518582857417168Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGCCGTATCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT32970.12364819556891671Illumina Single End PCR Primer 1 (100% over 33bp)
TCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATCCGC31010.11629755973891741No Hit
ACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATC28250.10594666438646942No Hit
ACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGA28130.10549662545810212No Hit
ATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGT27520.103208927572235Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)