FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20861683
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCG4278492.0508843893371402TruSeq Adapter, Index 27 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATC2130161.021087320711373TruSeq Adapter, Index 27 (97% over 39bp)
CGTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAT1311340.6285878277414148TruSeq Adapter, Index 27 (97% over 39bp)
CGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATC1287430.6171266239641356TruSeq Adapter, Index 27 (97% over 39bp)
TACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCT940490.4508217290043186TruSeq Adapter, Index 27 (97% over 39bp)
CGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCT777370.3726305303364067TruSeq Adapter, Index 27 (97% over 39bp)
CGTATGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG676360.3242116180175876TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA668950.32065965147682474TruSeq Adapter, Index 27 (97% over 39bp)
CGTATGCCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC581450.27871672673772296TruSeq Adapter, Index 1 (97% over 38bp)
CGTATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGA574010.275150379765621TruSeq Adapter, Index 27 (97% over 39bp)
CGTATGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC545100.2612924374318218TruSeq Adapter, Index 27 (97% over 39bp)
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT422260.20240936457523584No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC388370.18616427063914257Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC368050.1764239251454449Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTC366180.17552754492530637TruSeq Adapter, Index 27 (97% over 39bp)
ACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTC317040.15197239839182677TruSeq Adapter, Index 27 (97% over 39bp)
CGTATGCCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA297120.14242379198265068TruSeq Adapter, Index 1 (97% over 37bp)
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC283480.13588548920046384No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT266700.12784203460478236No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG264450.12676350225434832Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCAG214490.10281529059759943TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)