FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002439733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20861683
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGA2778411.3318244745642047Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGT2182101.0459846408365039Illumina Single End PCR Primer 1 (96% over 33bp)
ATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGT1045910.5013545647299885Illumina Single End PCR Primer 1 (96% over 33bp)
CATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG996850.47783776601341316Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTA949670.4552221409940895Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC665900.3191976409573475Illumina Single End PCR Primer 1 (96% over 33bp)
TACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTA616440.29548910315625065Illumina Single End PCR Primer 1 (96% over 33bp)
GGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG605180.29009164792696734Illumina Single End PCR Primer 1 (96% over 33bp)
GCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGT546310.26187244816249966Illumina Single End PCR Primer 1 (96% over 33bp)
GACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT509300.24413178936713784Illumina Single End PCR Primer 1 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC456830.21898041495501583Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC421300.20194919077238402No Hit
AGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG378990.18166798910711088Illumina Single End PCR Primer 1 (100% over 32bp)
CAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG366150.17551316449396726Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAG316420.15167520281081828Illumina Single End PCR Primer 1 (96% over 33bp)
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC315250.15111436598859257No Hit
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAG275180.13190690319664045Illumina Single End PCR Primer 1 (96% over 33bp)
AGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG274460.13156177284450157Illumina Single End PCR Primer 1 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG252140.12086273192819581Illumina Single End PCR Primer 1 (100% over 30bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT217320.10417184462058983No Hit
CGTATGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG216430.10374522515752924Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)