FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002439736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6574979
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT717551.0913342841095006No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC513420.7808694141836803No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA483390.7351962645051794TruSeq Adapter, Index 6 (97% over 36bp)
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT457700.6961238963652964No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT451760.6870896469783402No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC431020.655545819994254No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG373430.5679561866281246No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT343410.5222982461236758No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC261720.39805450329194964No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT225210.3425258088276784No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA218350.3320923154279276No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT216570.32938508244665116No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG216550.3293546640985469No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA166060.2525635443094191No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC148070.22520224018966448No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT129740.19732382415213798No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG129130.19639606453495895No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG127990.1946622186930179No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT118460.18016787582135244No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG114100.17353667593463037No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA96160.14625141768513633No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA94860.1442742250583614No Hit
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT93500.14220577738727377No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG91190.13869245818123524No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC85830.1305403408893017No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA83760.12739204186051392No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA77010.1171258493753364No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA76920.11698896680886738No Hit
CCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAT70200.1067684018458462TruSeq Adapter, Index 6 (97% over 36bp)
TACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGG69540.10576459635840661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)