FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002439739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2779974
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTG565172.0330046252231138Illumina Single End PCR Primer 1 (97% over 34bp)
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC361831.301558935443281No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC313091.1262335547023101No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATC254560.9156920172634707Illumina Single End PCR Primer 1 (97% over 34bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT217340.7818058730045676No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT201830.7260139843034503No Hit
CGGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGT176550.6350778820233571Illumina Single End PCR Primer 1 (97% over 34bp)
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT163830.5893220584077404No Hit
CGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGT162670.5851493575119767Illumina Single End PCR Primer 1 (97% over 34bp)
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG160550.577523386909374No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT160050.575724808937062No Hit
ACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTA157180.5654009713759913Illumina Single End PCR Primer 1 (97% over 34bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAG151620.5454007843238822Illumina Single End PCR Primer 1 (97% over 34bp)
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC123250.4433494701749009No Hit
CCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTG120820.4346083812294647Illumina Single End PCR Primer 1 (97% over 34bp)
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT120480.4333853482082926No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT113710.4090326024631885No Hit
CGGTGGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGG111810.402198006168403Illumina Single End PCR Primer 1 (97% over 34bp)
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC103740.3731689576952878No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT101540.36525521461711513No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC101000.36331275040701816No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG94550.34011109456419375No Hit
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGT72720.2615851802930531Illumina Single End PCR Primer 1 (97% over 34bp)
CGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTA62150.22356324195837804Illumina Single End PCR Primer 1 (97% over 34bp)
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG56210.20219613564731181No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG52270.18802334122549347No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA51030.1835628678541598No Hit
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAG50830.182843436665235Illumina Single End PCR Primer 1 (97% over 34bp)
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG48090.1729872293769654No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT46700.1679871826139381No Hit
CACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGT46530.16737566610335206Illumina Single End PCR Primer 1 (97% over 34bp)
AAGTGCAATTTCCGTTCTAAGTGCAATTTCCGTTCTAAGTGCAATTTCCG45810.16478571382322282No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG44000.1582748615634535No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA43930.1580230606473298No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC41340.14870642675075377No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT39470.141979745134307No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA38850.13974950844864018No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA34430.12385007917340234No Hit
CGGTGGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTG30760.11064851685663246Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)