FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002439740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14415942
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA558840.3876541678649928TruSeq Adapter, Index 2 (97% over 37bp)
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT327610.22725535382980866No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC246220.17079702457182472No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT219240.15208163295884514No Hit
CCAGTCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA209520.1453390975074678TruSeq Adapter, Index 2 (97% over 37bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT202020.14013652385671363No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG154770.10736030985696252No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC149510.10371157153656693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)