FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002439741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14415942
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC564760.39176073266665473Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC346990.24069880414335743No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC287160.19919613994007468No Hit
CGGTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCC210720.14617150929158843Illumina Single End PCR Primer 1 (96% over 33bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT187850.13030712803922215No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG179390.12443862496117146No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT162890.11299296292951234No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT156010.10822046870055387No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT150000.10405147301508288No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)