FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21764643
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT318758514.645703125017947No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT21611769.929756256511995No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC19889069.138243158870099No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG11393685.234949178812628No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG9580414.401822717698608No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT8320083.822750504108889No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC8259263.794806099047891No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA5355432.460610082141021No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT5080512.334295122598611No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA4454582.0467048322363937No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA3953591.8165195725930352No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC3675721.6888492037291858No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT3601901.6549318084381168No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG2769861.272642055282046No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC2017670.9270402459622242No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA1740360.7996271751390547No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA1688230.7756754843164668No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT1656650.7611657126652617No Hit
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT1430610.6573091963879215No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC1185260.54458049231499No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG1045600.480412198812542No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT1017250.4673864855031162No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA1003360.461004575172678No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG947360.43527477110467655No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT924100.4245877132007173No Hit
CGACGCTCTTCCGATCTATCGCGGATTAGCACTACGCATCGGTTACAAAC905490.4160371479559761No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC794820.3651886226665882No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT729130.33500664357324855No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT720750.33115636217878697No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC679370.31214387481568157No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG653020.300037083080113No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG649290.29832329434486937No Hit
CGACGCTCTTCCGATCTTCGCGGATTAGCACTACGCATCGGTTACAAACG539140.24771368866468427No Hit
TTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGA532010.24443773325388338No Hit
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC506060.23251472583308624No Hit
CGACGCTCTTCCGATCTCGGATTAGCACTACGCATCGGTTACAAACGAGG505130.23208742730124265No Hit
NTCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT417620.19188001383712106No Hit
TCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGT352010.16173479160673576No Hit
CGACGCTCTTCCGATCTCGCGGATTAGCACTACGCATCGGTTACAAACGA322700.14826799594185855No Hit
CGTGTGCTCTTCCGATCTATCGCGGATTAGCACTACGCATCGGTTACAAA315670.1450379866097505No Hit
TACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGG309020.14198257237667533No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG302320.1389041851042537No Hit
ACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATC298820.13729607235000363No Hit
NCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT283840.13041334976181324No Hit
GGCTAGCTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT283560.13028470074147322No Hit
CGACGCTCTTCCGATCTGATTAGCACTACGCATCGGTTACAAACGAGGAC283400.13021118701556464No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA282030.12958172573747248No Hit
TACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGAT278790.1280930727878238No Hit
CGACGCTCTTCCGATCTGGCTAGCGTCGAACTTACATCCGATCATGCTGT269280.12372360070413285No Hit
NGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC259590.11927142567879473No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA258850.11893142469646756TruSeq Adapter, Index 2 (97% over 37bp)
CGACGCTCTTCCGATCTATTAGCACTACGCATCGGTTACAAACGAGGACC257100.11812736831934252No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT221880.10194515940371729No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC217930.10013028929534934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)