FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21764643
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC371478917.067998772136995No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC16015527.358503422270698No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT15792917.256222856492524No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT9856104.528491462046954No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT9694124.454068003780259No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG8749814.020194588075715No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT7048063.2383071939199737No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT6222072.858797178524821No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT6059662.784176152119748No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT5581282.564379300868845No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC4697172.158165424537402No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC4548582.0898941462076817No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG3681611.6915554277641953No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG3398791.5616107280050493No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC3244891.490899712896738No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG3162461.4530263602302136No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA2345701.0777571678984121No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA1808260.8308245625715065No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA1582900.7272804796292777No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG1540040.707587990301518No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA1525930.7011049985979554No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC1442270.6626665091635089No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA1413180.649300794871756No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT1242500.570880027758783No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG1047510.48128976891557557No Hit
TTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGA781320.35898590204305214No Hit
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC729430.33514448180932715No Hit
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT541170.24864639406214933No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA469640.21578116397314673No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT467240.2146784580845181No Hit
GGTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT466630.21439818700449165No Hit
TCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGT448200.20593032470139758No Hit
TACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGAT424720.1951421854243141No Hit
GGCTAGCGTCGAACTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT387350.17797213581679242No Hit
GGCTAGCGTCGAATTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT349010.16035640924594996No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG329210.15125908566476373No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC316020.1451987978851755No Hit
GTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC265630.12204656883184346No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG265620.12204197422397418No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC265090.12179846000690202Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTAGCTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT264280.12142629676948986No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT255910.11758060998289749No Hit
ACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATG227640.10459165353642604No Hit
GACTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC219610.1009021834173894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)