FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9811890
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG1038621.0585320463233894TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA776570.7914581186703071TruSeq Adapter, Index 7 (97% over 38bp)
CCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT543840.5542663034338949TruSeq Adapter, Index 7 (97% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGT279560.2849196230287947TruSeq Adapter, Index 7 (97% over 38bp)
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT247430.2521736383102542No Hit
ACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA218730.22292341230894355TruSeq Adapter, Index 7 (97% over 38bp)
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC177020.18041376330146386No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT174090.17742759040307218No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT152270.1551892652689747No Hit
CACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATC148480.1513266047621814TruSeq Adapter, Index 7 (97% over 38bp)
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG132770.13531541833428626No Hit
CGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC128500.13096355544140834TruSeq Adapter, Index 7 (97% over 38bp)
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT128090.13054569506996103No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC127910.1303622441751793No Hit
GACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC124030.12640785822099512TruSeq Adapter, Index 7 (97% over 38bp)
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT101860.10381282301371092No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC98750.10064319922053752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)