FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9811890
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGA830350.8462691693445401Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC788950.80407546354474Illumina Single End PCR Primer 1 (97% over 35bp)
CGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTG578500.5895907923957565Illumina Single End PCR Primer 1 (97% over 35bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGAT282760.2881809722693589Illumina Single End PCR Primer 1 (97% over 35bp)
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC260410.26540248616729295No Hit
CGGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGT225350.22967032855036085Illumina Single End PCR Primer 1 (97% over 35bp)
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC218540.22272976969778505No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT175530.1788951975613261No Hit
CGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGT158190.16122276136401856Illumina Single End PCR Primer 1 (97% over 35bp)
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG155270.15824678018200367No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT144430.14719895962959226No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT133920.13648746571761405No Hit
CGGTGGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATA132780.13532561005066304Illumina Single End PCR Primer 1 (97% over 35bp)
CGGTGGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG126620.12904751276257684Illumina Single End PCR Primer 1 (97% over 35bp)
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT123380.12574539665650553No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT108650.1107329984335332No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC105190.1072066645671731No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT99090.10098971757734748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)