FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11578951
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT4141003.5763170601551044TruSeq Adapter, Index 7 (97% over 38bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG3799983.2817998797991286TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA1881651.6250608539581868TruSeq Adapter, Index 7 (97% over 38bp)
ACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA1430381.2353277943744645TruSeq Adapter, Index 7 (97% over 38bp)
CACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATC1189911.0276492231463799TruSeq Adapter, Index 7 (97% over 38bp)
GACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC824190.7118002312990184TruSeq Adapter, Index 7 (97% over 38bp)
CCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC604660.52220619985351TruSeq Adapter, Index 7 (97% over 38bp)
CGTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT525530.4538666758327244TruSeq Adapter, Index 7 (97% over 38bp)
CGTATGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC519460.4486244047496185TruSeq Adapter, Index 7 (97% over 38bp)
ACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCT517040.44653440540511835TruSeq Adapter, Index 7 (97% over 38bp)
CGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC465040.4016253285811469TruSeq Adapter, Index 7 (97% over 38bp)
CGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCT441450.3812521531527338TruSeq Adapter, Index 7 (97% over 38bp)
CGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA352630.30454399539301963TruSeq Adapter, Index 7 (97% over 37bp)
CGTATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA337700.2916499085279832TruSeq Adapter, Index 7 (97% over 38bp)
CGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATC331950.2866840009945633TruSeq Adapter, Index 7 (97% over 38bp)
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT311190.26875491570868554No Hit
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC278800.240781742663908TruSeq Adapter, Index 7 (97% over 38bp)
TGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA269460.23271538155744853Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTATGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC253500.21893174951686037TruSeq Adapter, Index 7 (97% over 38bp)
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC228050.1969522109558975No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT221510.19130403090919032Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT219620.1896717586938575No Hit
CGTATGCCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT219240.1893435769786054TruSeq Adapter, Index 7 (97% over 38bp)
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT193450.16707040214610114No Hit
GGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT184120.15901267740056935TruSeq Adapter, Index 7 (97% over 38bp)
GATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC183880.15880540473830487Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC177690.15345949732406675No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG176520.1524490430955274No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT171960.1485108625125022No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGT152690.13186859500484976TruSeq Adapter, Index 7 (97% over 38bp)
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT137330.11860314461992283No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC133960.11569269098729237No Hit
ATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG130170.11241951019569907Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT125530.1084122387252524No Hit
CCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTTCCAT119070.10283314956596673TruSeq Adapter, Index 10 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)