FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11578951
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTG4465563.8566187904241067Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGA3318912.8663304646508996Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC1842491.591240864565365Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGT1497291.293113685341617Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGT1275851.1018701089589202Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG850020.7341079515752333Illumina Single End PCR Primer 1 (96% over 33bp)
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAG624650.539470285347956Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTA544530.4702757615953293Illumina Single End PCR Primer 1 (96% over 33bp)
CATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTG505310.4364039540369417Illumina Single End PCR Primer 1 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTAT485830.41958032294980785Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTAT484700.4186044141649792Illumina Single End PCR Primer 1 (96% over 33bp)
TACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTA428720.3702580656917885Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTC364550.3148385376188223Illumina Single End PCR Primer 1 (96% over 33bp)
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC334270.28868763672978665No Hit
GCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGT331710.28647672833229887Illumina Single End PCR Primer 1 (96% over 33bp)
ATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGT328100.28335900203740394Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC312320.26973082449351415No Hit
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAG265330.22914856449431387Illumina Single End PCR Primer 1 (96% over 33bp)
GGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG250290.2161594776590729Illumina Single End PCR Primer 1 (96% over 33bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT226260.19540630234984152No Hit
GACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT211720.18284903356098492Illumina Single End PCR Primer 1 (96% over 33bp)
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG191480.16536903904334685No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT184600.15942722272509832No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT178890.15449586063538917No Hit
CGGTGGTCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCT173040.1494435894926924Illumina Single End PCR Primer 1 (96% over 33bp)
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT161180.13920086543245586No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGAT152070.13133314062733317Illumina Single End PCR Primer 1 (96% over 33bp)
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC145970.12606496046144422No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT134390.11606405450718292No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT124520.10753996627155603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)