FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002439752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13613122
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA361120.2652734618847903TruSeq Adapter, Index 7 (97% over 38bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG279810.20554432701036543TruSeq Adapter, Index 7 (97% over 38bp)
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT241050.17707179881293944No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC167360.1229402043116928No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT164940.1211625077627307No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT147360.10824849729547711No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)